Tài liệu Báo cáo khoa học: Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation doc

... compilation ª 2006 FEBS Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation Bernard ... affin- ity than with Mst1 (Fig. 3D). Both phosphorylation of Sav and its increase in abundance were dependent of the ability of Sav and Ms...

Ngày tải lên: 19/02/2014, 06:20

... that allows the receptors to fall into a constitutively active state without binding to a ligand are not automatically conserved within the MC receptors or within the MC1 receptors. Therefore, we ... region of each cMC1 receptor variant. To facilitate cloning and establishment of orientation of the PCR- amplified DNA fragments, recognition sites for HindIII and BamHI were intro...

Ngày tải lên: 21/02/2014, 00:20

... biphasic, with the best fit obtained with a two binding site model, comprising a first site with binding stoichiome- try (n) of 0.3 ± 0.2 and a binding constant (K d )of 3.4 ± 4 · 10 )9 m and a second with ... value of the protonable A B Fig. 3. Ensemble of the backbone traces of the 20 lowest energy conformers of the solution structure of (A) NarG(1–15) and (B) NarG...

Ngày tải lên: 16/02/2014, 14:20

... progress in Lea Sistonen’s labora- tory in order to unravel the specific targets of HSF1 in spermatogenesis and to estimate the proportion of com- R. Abane and V. Mezger Role of the HSF family ... instead of decreasing at 6 weeks. Such a high expression inhibits the maturation of olfactory sensory neurons and leads to a reduction in the thickness of the olfactory epit...

Ngày tải lên: 18/02/2014, 04:20

... behavior of this sequence could be due to enhanced interactions with Dicer and a reduced need to interact with TRBP, relative to the other sequences. Effect of guide strand 5¢ end mismatch on TRBP and ... duplex and directs binding of Dicer-2 to the other, less stable, end, and hence the guide strand is selected through interaction of its 5¢ end with Dicer-2 [1...

Ngày tải lên: 18/02/2014, 06:20

... conditions. All of the work with mice conformed to guidelines approved by the Institutional Animal Care and Use Committee of the University of Tokyo. Construction of the transgene Total RNA was ... mouse Correspondence H. Ichise, Laboratory of Gene Expression and Regulation, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirok...

Ngày tải lên: 18/02/2014, 17:20

... similar to that of Cg-Clp1. Expression of Cg-Clp2 transcripts during development and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue ... Double- stranded cDNA from oyster mantle edges was ligated to adaptors, and 25 ng of this template was used to PCR amplify 5¢- and 3¢-RACE fragments using adaptor-speci...

Ngày tải lên: 19/02/2014, 00:20

... mm EDTA to remove extracellular bivalent cations and then with Eriochrome Blue ⁄ buffer to remove EDTA. Cells were resuspended to an D 600 of 0.9–1.0 and treated with 10 lgÆlL )1 digitonin at room ... treatment with lambda phosphatase (kPP; New England Biolabs, Ipswich, MA). With the use of 0, 200, and 400 units of kPP, the samples were incubated for 30 min at 30 °C....

Ngày tải lên: 19/02/2014, 06:20

... Instituto Leloir, Buenos Aires, Argentina Trypanosoma cruzi, the etiological agent of Chagas’ disease, is a parasitic protozoan with a digenetic life cycle involving an insect vector and a mammalian host. ... 2006 FEBS to synthesize putrescine, as a result of the absence of ornithine and arginine decarboxylases (ODC and ADC) [7–10], which catalyse the first steps of both po...

Ngày tải lên: 19/02/2014, 07:20

... interactions with 14-3-3 and factor activating ExoS (FAS) protein cofactors [24–26]. As this interaction is necessary for the ADP-ribosyla- tion activity of ExoS, and more intriguingly appears to be ... a strategy of single amino acid site-directed mutagenesis of the cofactor interaction domain within ExoS. Various single mutant ExoS proteins were tested for their capacity to...

Ngày tải lên: 19/02/2014, 08:20