Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... there- fore the role of copper < /b> binding < /b> or loss of its binding < /b> could be related to specific cerebellar function(s) of stefin < /b> B [18], which remains to be seen by < /b> more in vivo studies. Stefin < /b> B as a copper < /b> binding < /b> ... 2006 The Authors Journal compilation ª 2006 FEBS 4263 High < /b> affinity < /b> copper < /b> binding...

Ngày tải lên: 19/02/2014, 06:20

... the bar graph, suggesting that the wild-type binding < /b> site on one receptor is not affected by < /b> the presence of a mutant mini-receptor that is incapable of binding < /b> PMP-BSA. Ligand binding < /b> by < /b> double-mutant ... that the percentage of binding < /b> based on contributions of the mutant (no binding < /b> activity) and wild-type (100% binding <...

Ngày tải lên: 18/02/2014, 08:20

... a p-cation -binding < /b> site, and an acyl -binding < /b> pocket) are the same in AChE and BuChE (Fig. 1). The acyl -binding < /b> pocket, which is responsible for the difference in substrate specificity between the two ... spontaneous reduction of DTNB from blank samples. The observed rate, in terms of mol productÆmin )1 ÆL )1 , was obtained by < /b> dividing the...

Ngày tải lên: 20/02/2014, 23:20

... Positions of DD and its subun- its (a D , b D and c D ) and the reactivase (DD-R) and its subunits (a R and b R ) are indicated on the right of the gels. In (C), to improve visi- bility of the bands of ... enzymeÆreactivase complexes by < /b> nondenaturing PAGE When the enzymeÆCN-Cbl complex was incubated with the reactivase in the presence of...

Ngày tải lên: 15/02/2014, 01:20

... troglitazone induces apopto- sis in prostate cancer cells by < /b> directly binding < /b> to Bcl-2 and Bcl-xL [40]. Owing to its inability to bind to Bcl-2 and Bcl-xL, pioglitazone cannot induce apoptosis in these ... troglitazone in the presence of Ac-DEVD-CHO (10 or 20 l M) for 96 h. (F) Inhibition of apoptosis by < /b> Ac-DEVD-CHO. AO ⁄ EB, apoptosis measured by < /...

Ngày tải lên: 16/02/2014, 09:20

... well characterized and modes of binding < /b> have been grouped into three main classes of PDZ binding < /b> motifs, occurring at the C-terminus of the interacting proteins [16]. We scanned the human olfactory ... PDZ domains could bind to GST alone. We then tested binding < /b> of single PDZ domains 1 + 2, and found that both could bind to the OR C-terminus (Fig. 2D)....

Ngày tải lên: 18/02/2014, 13:20

... Trong khi đó, trên b mặt gỉ thép đã được xử lý b ng chất B- 05 có cấu trúc mịn, đồng nhất, không b xốp rỗng (Hình 1 .b) . Như vậy b ớc đầu có thể thấy rằng sau khi được xử lý gỉ b mặt thép có ... hóa học b ng phổ hồng ngoại FTIR. Mẫu b t gỉ thép và gỉ thép đã được xử lý b ng chất biến đổi gỉ B- 05 trộn với b t KBr và nén thành viên với tỷ lệ 2%, được phân tích FTIR trong dải...

Ngày tải lên: 18/02/2014, 13:20

... different (Fig. 2B) . The C-domain parts of the sugar -binding < /b> sites of TvuCMBP and related proteins As the sugar -binding < /b> site is formed by < /b> N- and C-domains, residues involved in sugar binding < /b> are grouped into ... sugar -binding < /b> proteins. Evaluation of the binding < /b> affinities by < /b> fluorescence measurements The K d values of...

Ngày tải lên: 19/02/2014, 00:20

... five of the 11 cysteinyl sulfurs act as bridging ligands between two metal centers, and the remaining six act as terminal ligands by < /b> coordinating to a single metal center. The numbering of the ... investigated the metal -binding < /b> reactivity of the isolated a domain of human metallothionein isoform 1a, with specific emphasis on resolving the debate concerning...

Ngày tải lên: 19/02/2014, 00:20

... indicates that BAPTA action perturbs the tubulin asse mbly and disassembly balance on Fig. 2. Effects of BAPTA in the p re sence of other cytoskeleton drugs. (A) Immunostaining of interphasic RAT2 ... chelating units but in BAPTA the methylene links between oxygen and nitrogen are replaced by < /b> benzene rings. BAPTA is not protonated at physiological pH. The absence...

Ngày tải lên: 19/02/2014, 16:20