Tài liệu Báo cáo khoa học: Oligomerization of the Mg2+-transport proteins Alr1p and Alr2p in yeast plasma membrane pptx

... pres- ence of Alr1 oligomers, and split-ubiquitin assays reveal Alr1p Alr1p, Alr2p Alr2p, and Alr1p Alr2p interactions. These assays also show that both the N-terminus and C-terminus of Alr1p and Alr2p ... with the expression of ALR1-ALR1 Fig. 5. Interactions of Alr1p and Alr2p in the split-ubiquitin system. Alr1p and Alr2p were analyzed using the...

Ngày tải lên: 19/02/2014, 06:20

... picture of GH-57. In our alignment, in certain cases the extra N - and C-terminal ends were omitted. In the case of Q9Y8I8_ THEHY, the excised sequence corresponds to three regions that were originally ... domain (SLD2), a threonine-rich region and a putative transmem- brane domain [22]. Interestingly, the 3D structure of a protein domain, which is clearly homologous t...

Ngày tải lên: 19/02/2014, 12:20

... work- flow, and everything the user needs to perform an- notation tasks is included within the software. 2 The Project Window In the majority of cases, the annotator is interested in annotating a ... problems. Often, the annotation scheme has been built into the software, or the software has been limited in that they allow only certain types of annotation to tak...

Ngày tải lên: 20/02/2014, 09:20

... (long-distance move- ment of the N-domain and rotational motion of the A-domain) of SERCA during the ATP-mediated ion transport reaction [14,16–19]. The single line scanning method [8,15], in which an AFM ... Histograms of the height differences between the top of SERCA and the surface of the membrane. Statistical section analy- ses of SERCA were performed...

Ngày tải lên: 14/02/2014, 14:20

... control the amount of intracellu- lar CagA PY . Cortactin, ezrin and vinculin are all Src substrates, and Src inactivation causes dephosphoryla- tion of these proteins and is crucial for triggering ... distribution, making them unsuitable for the investigation of b-cate- nin signalling [29]. In support of this aspect, using Madin–Darby canine kidney cells that express E-...

Ngày tải lên: 14/02/2014, 19:20

... 37% of its original size) is typically in the range 2–3 mm [26]. Finding experimental evidence that electrical cou- pling is the key ‘spring’ interlinking the Ca 2+ stores has involved repeating ... junctions could serve as the spring joining the pendulums in the above analogy. However, coupling through release of Ca 2+ results in very weak coupling, as the effective d...

Ngày tải lên: 16/02/2014, 09:20

... subgroup of metzincins within the zinc proteinases family. The typi- cal multidomain structure of ADAM10 as a type I integral transmembrane protein consists of a prodo- main, a catalytical domain with ... delivery of furin into the brain of AD model mice increased a-secretase activity and reduced b-amyloid protein (Ab) production in infected brain regions [21], demon- stra...

Ngày tải lên: 16/02/2014, 09:20

... those of the wild-type protein that was encountered in the floating cells (Fig. 5C). Taken together, these data indicated that JSP1-wt, but not the -G2A mutant, induced detachment of cells and induction ... proteins are N-myristoylated. These proteins have a broad range of functions and include protein kinases and phosphata- ses, Ga proteins, nitric oxide synthase, ADP...

Ngày tải lên: 16/02/2014, 14:20

... factors, the members of the HSF family are modular proteins composed of functional domains (see figure 2 in [4]). The most con- served domain is the amino-terminal helix-turn-helix DNA-binding domain ... sig- nalling pathway originating from the membrane is unclear [67]. Furthermore, an impact of re-organiza- tion of membrane microdomains has been demon- strated both...

Ngày tải lên: 18/02/2014, 04:20

... between the silencing efficiency of the siRNAs and their binding to the RNA-induced silencing complex loading complex proteins HIV transactivating response RNA-binding protein and Dicer in H1299 cytoplasmic ... positioned after the PAZ domain binds to one end of the duplex [36,41], thus the reduction in binding affinity may result from the inability of the double...

Ngày tải lên: 18/02/2014, 06:20