Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc

... HsRad51 to examine whether these loops are actually involved in DNA binding. Because aromatic residues are involved in the ssDNA binding by bacterial single stranded DNA- binding protein (SSB) and ... to the Rad51- Y232W mutant, the Rad51- D231W, Rad51- S233W, and Rad51- G236W mutants did not cause significant defects in ssDNA binding and dsDNA binding A B...

Ngày tải lên: 19/02/2014, 06:20

... helix–span–helix domain at the C-terminus shown in blue medi- ates dimerization and, together with the basic domain, (red) DNA- binding. ‘*’, amino acids that are identical in all sequences in the align- ment; ... expres- sion in the face and limbs. In a follow-up study, they found that this conserved cis-acting sequence serves to maintain a level of AP-2a expression...

Ngày tải lên: 16/02/2014, 09:20

... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the HSF2 ... reduced methylation of the histone H3K9, irre- spective of the relative location of the HSF4 -binding regions and of the transcriptional status of genes l...

Ngày tải lên: 18/02/2014, 04:20

... (long-distance move- ment of the N-domain and rotational motion of the A-domain) of SERCA during the ATP-mediated ion transport reaction [14,16–19]. The single line scanning method [8,15], in which an AFM ... Representative single line scan graphs obtained at increasing ATP concentration in the range 0–100 l M and in the presence of 10 nM and 100 lM free Ca 2+...

Ngày tải lên: 14/02/2014, 14:20

... vinculin and ezrin, which are three well- known actin -binding proteins [6]. The phosphatases involved in this scenario, however, remain unknown and do not require Shp2. Instead, CagA PY can inhibit Src ... distribution, making them unsuitable for the investigation of b-cate- nin signalling [29]. In support of this aspect, using Madin–Darby canine kidney cells that express E-...

Ngày tải lên: 14/02/2014, 19:20

... typically in the range 2–3 mm [26]. Finding experimental evidence that electrical cou- pling is the key ‘spring’ interlinking the Ca 2+ stores has involved repeating the decoupling experiment of Fig. ... composed of a large number of Ca 2+ store oscillators. In this simulation, the intrinsic frequencies of oscilla- tors are different from each other, and as the [Ins(...

Ngày tải lên: 16/02/2014, 09:20

... ADAMs lack the RX(6)DLPEFa(9)b(1) integrin binding motif, which is contained in the other members of the pro- teinase family [48]. Nevertheless, there are significant differences between ADAM10 and TACE ... transient inhibitor, but also as an internal chaperone in the mat- uration of the enzyme. Accordingly, the viral delivery of furin into the brain of AD model m...

Ngày tải lên: 16/02/2014, 09:20

... myristoylation interferes with binding of the signal recognition particle, resulting in a part of NADH-cytochrome b5 reductase escaping the endo- plasmic reticulum insertion pathway and relocating to the ... can also in uence the movement and final destination of a signaling protein within the cell. We observed that myristoylation of JSP1 deter- mined its localizatio...

Ngày tải lên: 16/02/2014, 14:20

... associated with the generation of heterochromatin, and reduced the binding of HSF1. These results show that HSF4 promotes the DNA- binding activity of another mem- ber of the HSF family, through modulating the ... stress-inducible Hsp70i gene during mitosis and to enhance the DNA- binding capacity of HSF2 [96,98]. However, another report showed that the mod...

Ngày tải lên: 18/02/2014, 04:20

... determine whether the phosphorylation-induced gain in hydrophobic surface area described here affects other properties of HSL than binding to lipids, e.g. binding to lipid droplet-associated proteins. In ... exposes and ⁄ or increases the lipid -binding area of the enzyme. A direct demonstration of this presumed conformational change will have to await the solving of...

Ngày tải lên: 18/02/2014, 11:20