Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

... oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level Yongzhao Zhang1, Kazumichi Furuyama1, Kiriko Kaneko1, Yuanying Ding1, Kazuhiro ... regula-tion of HO-2 expression in human cells under hypoxia. In the present study, we have analyzed the effect of hypoxia on the expression levels of HO-1 and HO-2 in various types of human cell line, including ... adaptation to hypoxia in certain cell types, which may contrib-ute to the maintenance of the intracellular heme level. AbbreviationsHO, heme oxygenase; HRE, hypoxia response element; MARE, Maf...

Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx

... protective agent against DNA damageunder conditions of tumor hypoxia. Many strategies for cancer therapy are based on increases in the number of DNA strand breaks (e.g. radiotherapy and alkylat-ing agents). ... extracellularcontent and the increase of its uptake induced by hypoxia show that alteration of pyruvate metabolismtakes place. The greater uptake might be due to anincreased need to maintain antioxidant capacity ... CytosolGlutamate5-Oxoprolineγ-Glutamyl-cysteineCysteine GlycineADP + Pi + ATP + ATPADP + Pi GlutamineGS-X(conjugate)Amino acids (AA)γ-Glutamyl-AACysteinyl-glycineγ-Glutamyl-AACysteinylglycineconjugateExtracellular are a 2×GlutathioneGSHFig....

Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc

... expression, we used the following validated siRNAs: Hs_MAPK14_6_HP validatedsiRNA against human p3 8a; Hs_MAPK3_7_HP validatedsiRNA against human ERK1; and Hs_MAPK1_10_HPvalidated siRNA against ... denaturedat 90 °C, cooled for annealing, and used to knock downHIF- 1a. Similarly, the siRNA against HIF- 2a was preparedusing the sense (5¢-CAGCAUCUUUGAUAGCAGUdTdT-3¢) and antisense (5¢-ACUGCUAUCAAAGAUGCUGdTdT-3¢) ... interfering RNAs(siRNAs) against HIF- 1a, HIF- 2a, and HIF- 3a, the levels of HIF- 1a and HIF- 2a in cells under hypoxia were drastically decreased (Fig. 4A) . In the case of HIF- 3a, endogenous HIF- 3a was not...

Tài liệu Báo cáo khoa học: Hypoxia-inducible factor-1a blocks differentiation of malignant gliomas pdf

... corresponding to the targeting siRNAsis: HIF- 1a: 5¢-TCGACAAGCTTAAGAAAGA-3¢; VHL:5¢-CCAAGACACCTCGAGAAT-3¢ (Ribobio, CN). The scrambled siRNA sequence used as negative control is5¢-GAGUAGAAGAUUCAAGCUU-3¢ ... Statisticalevaluation revealed that the amount of HIF- 1a wassigniﬁcantly increased in parallel with increasing gliomagrade (Fig. 7B). The percentage of HIF- 1a- positivecells in Grade I averaged ... expression increases with tumor gradeand inhibits differentiation induced by forskolin in primary human malignant glioma cellsMalignant gliomas are a spectrum of tumors of varying differentiation and...

Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding afﬁnity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx

... al. [34]. The protein conce-ntration was calculated based on the values obtained for alanine, threonine, glycine, valine, phenylalanine and aspar-tate. The analysis, starting from protein hydrolysis, ... surface of the structural unit that contains the catalytic site, ratherthan on an auxiliary domain [9]. These substrate bind-ing sites are located at a certain distance from the active site and ... degrad-ing enzymes. Human salivary a- amylase contains sev-eral SBS. Mutational analysis demonstrated that theseSBS residues are important for the activity on starchand that they play a role in...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: histone H3 lysine 4 methyltransferases from yeast to human pptx

... overexpressed in prostate can-cer. The implication of HMTs and demethylases in cancer and human diseases has led to the idea of utiliz-ing them as therapeutic targets. In fact, biguanide andbisguanidine ... andbisguanidine polyamine analogs inhibit the demethy-lase, LSD1, in human colon carcinoma cells. Inhibition of LSD1 facilitates expression of the aberrantlysilenced genes in cancer cells [156]. In addition ... of the fundamentalSET domain in these HMTs is intriguing. In addition,many of the H3K4 HMTs share several domains ⁄ com-ponents, indicating a common mechanism of action.Concluding remarksStudies...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... protein seems to have severalfunctions, both intracellularly and extracellularly. Byreducing the S10 0A4 level in a human osteosarcoma cell line, and implementing these in mice, the capacityto ... I,Iwata K, Yamashita K & Hayakawa T (1992) Matrixmetalloproteinase 9 (92-kDa gelatinase ⁄ type IV collage-nase) from HT1080 human ﬁbrosarcoma cells. Puriﬁca-tion and activation of the precursor ... they inter-act with ﬁbrillar 3D collagen I are various human tumour cell lines [21,22], human skin ﬁbroblasts[21,23–27], human umbilical vein and neonatal foreskinendothelial cells [28], human...

Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... polyclonal anti-bodies raised against each of the WhiB proteins. The absorbanceobtained in the positive control (where the antibody used wasraised against that particular antigen) was set to ... pH andredox conditions [1]. Among several mechanisms for resistance to intracellular killing, the scavenging of freeradicals and the reactivation of degenerated proteinsduring infection by a ... MS was used to determine the sta-tus of cysteines after removal of the iron–sulfur cluster.Reaction of IAA with a cysteine-thiol causes anincrease in molecular mass of 57 Da, therefore, the 015304560757590105120Effect...

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... andfunctional properties of RNase and participates in degradation of IL-1b mRNADanuta Mizgalska1, Paulina We˛ grzyn1, Krzysztof Murzyn2, Aneta Kasza1, Aleksander Koj1,Jacek Jura3, Barbara ... following antibodies were used: polyclonal antibodiesagainst lamin C (Abcam, Cambridge, MA, USA) for the nuclear fraction, polyclonal antibodies against GAPDHD. Mizgalska et al. MCPIP protein as ... pcDNA3 at the same restriction sites. Then a second fragment situated after the PIN domain was gen-erated by PCR using primers 5¢-AGCGACCTGAGACCACTCACTTTGGAGCAC-3¢ (BsaI) and 5¢-GGAATTCCCTCACTGGGGTGCTGG-3¢...

Tài liệu Báo cáo khoa học: PC1⁄3, PC2 and PC5⁄6A are targeted to dense core secretory granules by a common mechanism doc

... triad of amino acids asparticacid, histidine and serine, and a stabilizing P-domaininvolved in the binding of Ca2+[1]. The C-terminaldomains of the PC enzymes exhibit the least amount of homology ... extent of intracellular protein retention and forskolin-stimulatedsecretion of the Fc protein thereby constitute the base-line for analyzing potential granule-sorting domains.Attachment of the ... 25 amino acids of PC2, which havebeen reported to form an amphipathic a- helix capable of interacting with raft resident lipids [6]. The granule-targeting domain of the PC5 ⁄ 6A pro-tease has...

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