Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

... reduces levels of mutant huntingtin and the risk of neuronal death. Nature 431, 805–810. 97 Selkoe DJ (2004) Cell biology of protein misfolding: the examples of Alzheimer’s and Parkinson’s diseases. Nat Cell ... Trautinger F (1996) Modification of growth and tumorigenicity in epidermal cell lines by DNA- mediated gene transfer of M r 27,000 heat shock protein (hsp27...

Ngày tải lên: 19/02/2014, 18:20

... reactions of trans-sulfura- tion through the production of homocysteine, biologi- cal methylations of DNA, lipids and proteins, and polyamine biosynthesis through the production of methionine and S-AdoMet ... result of this approach indicated the orienta- tion of 5-methyltetrahydrofolate in the MetS active site. Figure 5 shows the superimposed structures of DHPS and 5-m...

Ngày tải lên: 19/02/2014, 05:20

... not shown). Inhibition of pneumococcal murein hydrolases by bicyclic amines As shown above, tropic esters of bicyclic amines were selected and characterized by biophysical methods as strong ligands ... analysis of ligand binding to C-LytA. (A) Con- formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon additio...

Ngày tải lên: 19/02/2014, 05:20

... [51,52]. The O of Ser90 and the S of Cys266 of pea FNR are close to N5 and O4 of the isoalloxazine, which are involved in hydride transfer. The hydroxy group of Ser90 could accept a hydrogen bond and thus ... for appropriate binding of the metal ion, which remains accessible from the exterior (Fig. 7B). Distances between the N5 and O4 of the flavin, O of Ser90, S of C...

Ngày tải lên: 19/02/2014, 16:20

... inactivation of G APDH being metal- ion independent, whereas inhibition of GR and LDH by H 2 O 2 occurs most rapidly in the presence of metal ions. It is proposed that the inhibition of GAPDH and GR by these 1 O 2 -generated ... its 1 D g state; 1 O 2 )is generated by a number of enzymatic and chemical reactions, by UV exposure, and by visible light in the pres...

Ngày tải lên: 21/02/2014, 03:20

... BTK–SYK and ITK– SYK fusion proteins lead to differential phosphorylation levels of the fusion proteins themselves, as well as the downstream adapter proteins SLP76 and BLNK, in 293T and COS7 cells. ... ª 2011 FEBS MINIREVIEW TEC family kinases in health and disease – loss -of- function of BTK and ITK and the gain -of- function fusions ITK–SYK and BTK–SYK Alamdar Hussa...

Ngày tải lên: 14/02/2014, 18:20

... all the cell lines tested and the efflux of rhodamines in MRP1 over-expressing cells was abolished by NBD-Cl, it appears that rhodamines are excluded from these cells by MRP1. On the other hand, ... SD of four independent experiments. Fig. 4. The effect of MK571 and NBD-Cl on TMRM distribution in K562 and 2008 cell lines. K562 cells and 2008 cells were incubated in the ab...

Ngày tải lên: 18/02/2014, 13:20

... the three concentrations tested, inhibition of MTT by Ab(1–40) and Ab(M1– 40) was essentially identical; similarly, the degrees of inhibition caused by Ab(1–42) and Ab(M1–42) were indistinguishable ... E22K, E22Q and D23N), and the availability of a rapid and simple expression and purification protocol will facilitate large-scale investigations of the molecular dete...

Ngày tải lên: 18/02/2014, 13:20

... proteomics. Characterization of chloroplast proteins of Arabidopsis thaliana by top-down mass spectrometry. Mol Cell Proteomics 2, 1253–1260. 21 Park JH, Burns K, Kinsland C & Begley TP (2004) Characterization of ... nozzle-skimmer dissociation spectra of 1 : 1, 2 : 1 and 3 : 1 mixtures of 144 and 116 kDa proteins showed the corresponding molecular ions and, for eac...

Ngày tải lên: 18/02/2014, 16:20

... study addresses the reactivity of aldehydes with cysteine lig- ands of zinc in proteins. Moreover, it demonstrates that aldehydes release zinc from isolated proteins and in cultured cells and that the released ... proposed mechanism of activation involves a reaction of electrophiles with the cysteine ligands of Keap1, followed by zinc release [28]. The reactions of aldehy...

Ngày tải lên: 19/02/2014, 06:20