Tài liệu Báo cáo khoa học: An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina pdf

... An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina Tom Desmet 1 , Tineke Cantaert 1 , Peter Gualfetti 2 , Wim ... a better understanding of the factors that contribute to the divergent specificity of these xyloglucanases. Experimental procedures Enzyme expression and purification Cel74A was obtained from an e...

Ngày tải lên: 19/02/2014, 05:20

... The Authors Journal compilation ª 2009 FEBS 121 pro-IL-1b in the cytosol and the appearance of IL-1b in the cell supernatants (Fig. 2C). A lower level of IL-1b was found in the supernatants of ... shock and multiorgan failure [3–6]. The role of LeTx in anthrax pathogenesis is complex, however, and probably involves the impairment of the innate and adaptive immune res...

Ngày tải lên: 16/02/2014, 09:20

... first left brother of the parent 0.2505 1.5603 0.6145 5.2 The analysis to the influence of the structural relation and the structural distance to the predictive information quantities of the history ... the influence of the structural relation and structural distance between the current node and the node that the given feature type related to has to the predi...

Ngày tải lên: 20/02/2014, 18:20

... describe the domain in which the di- alogue system operates (an “in-car” information sys- tem). Then we describe the major components of the system and give examples of their use. We then discuss the ... describes the main components and functionality of the system, as well as the pur- poses and future use of the system, and surveys the research issues involved in...

Ngày tải lên: 22/02/2014, 02:20

... in the case of similar train and test corpora, where the mean of the distribution (8.50) is close to the median (8.0), but not in the case of divergent corpora, where the mean (6.04) and median ... range of techniques, from interpolation at either the count level or the model level (Bacchiani and Roark, 2003; Bacchiani et al., 2006) to using explicit models of synt...

Ngày tải lên: 20/02/2014, 04:20

... are the largest class among the antecedents of Com- mit, even if they only account for 43% of such an- tecedents. Another indirect source of evidence for hypothesis 1.1 arises by exploring the ... Directive + Offer and if the associated solution size is determinate. To gain some evidence in support of 1.1, we checked whether the hypothesized WFPs appear as antecede...

Ngày tải lên: 20/02/2014, 18:20

... from the reaction were analyzed by HPLC. In the absence of a metal chelator, RPE65c cat- alyzed the production of 11cROL and 13cROL from atRE (Fig. 4A). However, in the presence of 1 m M of the ... spans the entire thickness of the inner retina. The Mu ¨ ller cell constitutes an ana- tomical link between the retinal neurones and supports their activities by e...

Ngày tải lên: 14/02/2014, 14:20

... equivalent to the Meis2a splice variant, and these two proteins share 74% identity and 80% similarity over their C-terminal domains. Comparison of the Meis2d isoform analyzed here with the public ... con- text of the GBD fusion proteins, which contain an nuclear localization signal within the GBD part of the protein. Another possible explanation for the observed autoinhi...

Ngày tải lên: 16/02/2014, 15:20

... that corresponded to the respective 5¢-terminus of the substrate DNA. The letters indicate the 3¢-termini of these radiolabeled marker DNAs. (C, D) Schemes for the substrate cleavage sites of PL DNAzyme and DRc ... (stems I and II) flanked by regions of ssDNA [21]. The 5¢-arm of the enzyme binds the cleavable sequence via Watson–Crick base pairs and the 3¢-arm t...

Ngày tải lên: 16/02/2014, 15:20

... achieved. Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H] + ) (Fig. 3A). Amino acid sequence analysis of the ... Aliquots of the input and the eluate of the chromatography were resolved by Tricine ⁄ SDS ⁄ PAGE and then analyzed by Coo- massie Brilliant Blue st...

Ngày tải lên: 18/02/2014, 13:20