... An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina Tom Desmet1, Tineke Cantaert1, Peter Gualfetti2, Wim ... abetter understanding of the factors that contribute to the divergent specificity of these xyloglucanases.Experimental proceduresEnzyme expression and purification Cel74A was obtained from an engineered ... 4200116976645Fig. 1. SDS ⁄ PAGE analysis of Cel74A: lane 1, molecular massmarkers (kDa); lane 2, Cel74A; lane 3, Cel74A treated with EndoH;lane 4, EndoH.T. Desmet et al. Hypocrea jecorina Cel74A FEBS Journal...