Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

... ª 2006 The Authors Journal compilation ª 2006 FEBS Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling Sandra Kleiner, Amir Faisal* and Yoshikuni ... clear that the expression of E-cadherin and the expression of MMPsare inversely correlated [11,12] and that E-cadherin- dependent cell–cell contact regulates the expression of MMPs and uPA in vitro ... inducer of uPA gene expression [16].Decma-induced uPA gene expression isdependent on Erk activationWe and others have shown that activation of Erk playsan important role in uPA gene expression...

Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf

... to the sequence of the hsp70 promoter between )206 and )187 bp, and that mutations of this sequence abrogated the activation of the hsp70 promoter by Hsp105b. In addition, overexpression of Hsp105bstimulated ... Hsp105bstimulated the phosphorylation of STAT3 at Tyr705 and its translocationto the nucleus. Downregulation of STAT3 expression resulted in reduction of the activation of the hsp70 promoter by Hsp105b. Furthermore, ... between )206 and )187 bp of the hsp70 gene is essential for activation of the hsp70 promoter by Hsp105bTo identify the hsp70 promoter region that is necessaryfor the induction of Hsp70 by Hsp105b,...

Tài liệu Báo cáo khoa học: An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... Model of the 3D structure of the neutral protease from Bacillus stearothermo-philus, including the position of the disulﬁdebridge in mutant G8C ⁄ N60C. The modelwas created on the basis of the ... ﬂuorescence and CD spectra of the unfolded and the degraded protein are nearly identical [15], the progresscurves in the presence of GndHCl reﬂect the decrease of native molecules, and autoproteolysis ... the presence of 2–5mm CaCl2, by the addition of isopropanol leads to areduction of the observed rate constants of unfolding. The kobsvalues coincide for both of the enzymes and produce straight...

Tài liệu Báo cáo khoa học: "From HOPE en I''''ESPERANCE On the Role of Computational Neurolinguistics in Cross-Language Studies " pptx

... develop a broader perspective of the questions within an analysis of the hypothesis in the con- text of a characterization of the "how" of the entire behavior. By adapting HOPE for processing ... process time interval, there are six types of serial-order process that can occur and affect the state of the process. The most important aspect of the control is that all of the serial order ... both aspects of the process is the focus of comparison between "normal" and "lesion" performance of the model. In this way we are able to study the effect of the "lesion"...

Tài liệu Báo cáo khoa học: "Language Resources Factory: case study on the acquisition of Translation Memories" potx

... rest of the input data if the workﬂow is robust (i.e. makesuse of retry and parallelisation) and the error isconﬁned to a WS (i.e. it does not affect the rest of the workﬂow).An instance of ... and one of my-Experiment have been deployed to be the Reg-istry and the portal to share workﬂows and otherexperiment-related data. Both have been adapted by modifying relevant aspects of the ... On the other hand, GrAFcan be used as a pivot format between other for-mats (Ide and Bunt, 2010), e.g. there is softwareto convert GrAF to UIMA and GATE formats (Ide and Suderman, 2009) and...

Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... reference, the position of the sugar and of the metal in the crystallo-graphic template 1YOE. The two ligands were modelled ineach binding site of SsCU-NH, and then the structure of the resulting ... pro-trudes towards the exterior of the tetramer near loop275–288 of the opposite monomer. Therefore, the binding and adaptation of this portion of SsCU-NHto the active site of the protease could ... obtained by optimiz-ing both the growth time of the transformed cells and the induction time with isopropyl thio-b-d-galactoside(IPTG). The most favorable conditions for the expression of the enzyme...

Tài liệu Báo cáo khoa học: Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity docx

... without the need for PI-3¢ kinase activity asjudged from the absence of an effect of arsenite onPI-3¢ kinase activation and the lack of inhibition by eitherwortmannin or LY 294002. Furthermore, ... uptake. Another early target of insulinaction, the activation of ERK- 1, -2 was also not activatedappreciably in response to arsenite-treatment either(Fig. 3D). The effect of arsenite on Cbl and caveolin-1 ... arsenite-induced glucose uptake (Fig. 5C). Thesedata illustrate the distinct nature of the insulin- (namely the IR) and arsenite-induced tyrosine kinases, and fur-thermore they suggest that tyrosine kinase...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... THP-1monocytes. In addition, the effects of KLF4 on the expression of SR-BI and the primary mechanism werealso investigated.ResultsHDL induces the expression of KLF4 and SR-BI inPBMC and phorbol 12-myristate ... demonstrated the increasing expression of KLF4 in PBMC and THP-1 cells, and identiﬁed that induction of KLF4 by HDL3promoted the expression of hSR-BI. It has been shown that dis-ruption of SR-BI ... bindingelement on the promoter of SR-BI. All results indicate a potential function of KLF4 in the pathogenesis of atherosclerosis through the regulationeffect on atherosclerotic-related genes.AbbreviationsChIP,...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... of this type exhibit the predictedamount of binding only if the heterodimer is tethered by the wild-type partner. By contrast, we found that, if the heterodimer is tethered to the resin by the ... employing solubleforms of the receptor [25,26,34], indicates that the ectodomain of the receptor is capable of dimer forma-tion in the absence of ligand.Afﬁnity cross-linking of [125I]IGF-II to ... between repeats 11 and 13because repeat 11 is capable of binding IGF-II even in the absence of repeat 13 [20,22]. These data suggestthat the failure to tether the tail of the extracyto-plasmic...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... signiﬁcantlyincreases the cost of the puriﬁcation, and the require-ment for HPLC increases the length and complexity of the puriﬁcation procedure. Moreover, as with the studies by Lee et al. [21] and Subramanian ... E22K, E22Q and D23N), and the availability of a rapid and simple expression and puriﬁcation protocol will facilitatelarge-scale investigations of the molecular determinants of aggregation and ﬁbrillation. ... Subramanian and Shree[42], there was no rigorous assessment of the purity of the product or the correctness of the sequence. In con-trast, the puriﬁcation protocol that we have developedis quick and...

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