Tài liệu Báo cáo khoa học: Dietary antioxidant curcumin inhibits microtubule assembly through tubulin binding pptx

... 5320–5332 ª 2006 The Authors Journal compilation ª 2006 FEBS Dietary antioxidant curcumin inhibits microtubule assembly through tubulin binding Kamlesh K. Gupta 1 , Shubhada S. Bharne 2 , Krishnan ... curcumin might be composed of tubulin curcumin complexes. To analyze whether curcumin could incorporate into the tubulin polymer as tubulin curcumin complex, we first...

Ngày tải lên: 19/02/2014, 05:20

Ngày tải lên: 18/02/2014, 03:20

... properties of their binding sites. HSF4 binding was shown to be closely associated with reduced methylation of the histone H3K9, irre- spective of the relative location of the HSF4 -binding regions ... of HSF4 -binding regions also reveals new features because, in contrast to the classical heat shock genes, these HSEs are not only found in the promoter regions. Actually, only 5% of HSF4...

Ngày tải lên: 18/02/2014, 04:20

... Sarraf SA & Stancheva I (2004) Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chroma- tin assembly. Mol Cell 15, 595–605. 51 Ichimura ... mechanistically mediated by the state of chromatin modification. DNA methylation attracts binding of methyl- CpG -binding domain proteins that trigger repression of transcription, whereas...

Ngày tải lên: 18/02/2014, 14:20

... activity by OA pro- duces the abnormal hyperphosphorylation of tau that inhibits its binding and the promotion of microtubule assembly in vitro [28]. In primary cultures of cerebellar granule cells, ... composed of a- and b-tubulins. Microtubules are stabilized by specific factors, including micro- tubule-associated proteins, such as tau, and post-trans- lational modifications (a -tubul...

Ngày tải lên: 18/02/2014, 14:20

... Protein denaturation, Part C. Adv. Protein Chem. 24, 1–95. 55. Schellman, J.A. (1975) Macromolecular Binding. Biopolymers 14, 999–1018. 56. Alexov, E. (2003) The role of the protein side chain fluctuations ... given titratable group is 0.5 is pK ½ . To adopt a common nomenclature, pK ½ will be referred as pK a throughout the text. Optimum pH, isoelectric point (pI) and bases/acids ratio The e...

Ngày tải lên: 19/02/2014, 12:20

... complex [20]. Hence, the mechanism of binding of fosfomycin by reinforcement of an initial binding site (mainly K22 and R397) through recruit- ment of secondary binding partners located in a flexible loop ... fosfomycin binding due to charge–charge repulsions. Most interestingly, the uncharged valine side chain completely abolishes fosfomycin binding in the absence of UDPNAG. Clear...

Ngày tải lên: 19/02/2014, 13:20

... temperature o f lysozyme. This data could be interpreted in terms of A rgEE binding to unfolded lysozyme, possibly through the ethyl- ated carboxyl group, which leads to effective prevention of intermolecular ... increase in the apparent net charge of the unfolded molecules. The increased net charge caused by binding of the additives would effectively increase the electrostatic repulsion...

Ngày tải lên: 19/02/2014, 16:20

... vertebrate DNases I) and insertion of an additional Thr or Asn residue into an essential Ca 2+ - binding site. Site-directed mutagenesis of shark DNase I indicated that both of these alterations ... DNase I family may have acquired high stability and effective expression of the enzyme p rotein through structural alterations in both the mature protein and its signal sequence regions during...

Ngày tải lên: 19/02/2014, 16:20

... myristate binding to HSA. Binding sites are indicated with the notation (FA1)P(FAx)–(FAS), where FA1 is the heme binding site, acting as myristate binding site as well, FAx is a different myristate binding site ... sites FA6–7 are low-affinity fatty acid binding sites [1,6,7,9–17]. The fatty acid binding site FA1, located in subdo- main IB (Fig. 1), acts as the heme binding sit...

Ngày tải lên: 20/02/2014, 02:22