Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... Authors Journal compilation ª 2006 FEBS Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori Cong Han 1 , Lirui Wang 1 , Kunqian Yu 1 , Lili ... expressed, and purified in Escherichia coli system. The enzymatic characterization of HpSDH demonstrates its activity with k cat of 7.7 s )1 and K m of 0.148 mm towa...

Ngày tải lên: 19/02/2014, 05:20

... human and pufferfish (torafugu and spotted green pufferfish) IL-10 sequences. Pufferfish and carp IL-10 genes, clustered together and distant from IL-20 and IL- 24, as recently determined from analysis ... activities of IL-10, suppressing the immune system of the host to facilitate its survival [7]. As innate immunity is known to be important in the defense of pathogens, isol...

Ngày tải lên: 20/02/2014, 02:21

... fluorescence intensity as function of time of double- labeled liposomes made from PS (A) or from PC (B) after the addition of MfGl-IIorLPSReatt = 50 s and subsequent addition of LBP (0.2 m M )att = 100 ... activation of protein kinase C, secretion of nitric oxide and prostaglandin E2 as well as augmented glucose utilization and lactate formation [11]. These data were supp...

Ngày tải lên: 21/02/2014, 00:20

... sequence of the IgE-reactive protein. All chemicals were from Applied Biosystems. Cloning the cDNAs of two isoforms of b-fructofuranosidase from tomato fruit Total RNA was isolated from tomato ... map and glycan analysis of the natural b-fructofuranosidase Investigation of the carbohydrate moieties of the purified allergen from tomato was carried out by MALDI-TOF mass spe...

Ngày tải lên: 21/02/2014, 00:20

... Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai Ken-ichi Suzuki, Takao Ojima and Kiyoyoshi Nishita Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries ... domain. Cellulose-binding ability and other biochemical functions Fig. 4. Structures of cDNA and genomic fragment for HdEG66. (A) structure of HdEG66 cDNA. Open...

Ngày tải lên: 20/02/2014, 23:20

... evolved from a common ancestral gene, and the essence of most of the conserved amino acids has been further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand ... initially from Ruta species. Flavonols originate from flavanones, i.e. (2S)-naringenin, by the consecutive action of FHT and FLS (Fig. 1), and both of these enzymes use mo...

Ngày tải lên: 21/02/2014, 03:20

... crotonyl-CoA, 3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoA were used for the kinetic characterization of AUH. The data are summarized in Table 2. Overexpression of a mutant form of AUH and its activity on ... Peak 3 produced small amounts of HMG-CoA and large amounts of free CoA. Peak 4 produced HMG-CoA and also large amounts of free CoA. Peak 5 produced large amounts...

Ngày tải lên: 19/02/2014, 07:20

... oxidase from spinach [EC 1.1.3.15 (GOX)] [44], L -lactate dehydrogenases from bacteria (EC 1.1.1.27) [45] (S)-mandelate dehydrogenase from P. putida (MD) [46] and inosine 5¢-monophosphate dehydrogenases ... signals of DMAPP and IPP. A [3,4,5- 13 C 3 ]DMAPP; B, mixture of [3,4,5- 13 C 3 ]DMAPP and [3,4,5- 13 C 3 ]IPP obtained from [3,4,5- 13 C 3 ]DMAPP by the catalytic acti...

Ngày tải lên: 19/02/2014, 13:20

... graph of R 1 , R 2 and { 1 H}– 15 N NOE val- ues along the sequence of the TTF1 HD at 11.7 T and 286 K. Fig. S2. Plot of J(0.87x N )–J(0) correlation from 15 N relaxation measurements of the ... and Ala60–Gln67), together with those from Arg58 and Gln59 in the C-terminal end of helix III, are located apart from the major cluster, towards the lower intercept of the th...

Ngày tải lên: 18/02/2014, 16:20

... Effect of class-specific protease inhibitors pepstatin (Peps., inhibitor of aspartyl proteases), AEBSF (inhibitor of serine proteases), E64 (inhibitor of cysteine pro- teases) and leupeptin (inhibitor ... nature of the molecule, as assessed from the salt and heparin dependence of the antimicro- bial and antifungal effects. Results Antimicrobial effects of recombinant...

Ngày tải lên: 18/02/2014, 17:20