Tài liệu Báo cáo khoa học: Bacterial-induced hepoxilin A3 secretion as a pro-inﬂammatory mediator pptx

Tài liệu Báo cáo khoa học: Deciphering enzymes Genetic selection as a probe of structure and mechanism docx

... thebifunctional enzymes chorismate mutase–prephenate dehydratase and chorismate mutase–prephenate dehydratase were deleted. Monofunctionalversions of the dehydratase and the dehydrogenase are provided ... interactions may be an easy way toincrease enzyme stability. This hexameric variant, however,suffered a 200-fold decrease in catalytic efﬁciency. Incontrast, the unstable monomeric variants had ... andfunctionally viable, then complete sampling becomesfeasible for libraries in which a larger number of aminoacids are varied simultaneously. Primordial protein catalystsmay have had to manage with...

Tài liệu Báo cáo khoa học: "Using an Annotated Corpus as a Stochastic Grammar" ppt

... (ATIS) spoken language corpus. Preliminary experiments yield 96% test set parsing accuracy. 1 Motivation As soon as a formal grammar characterizes a non- trivial part of a natural language, ... Using an Annotated Corpus as a Stochastic Grammar Rens Bod Department of Computational Linguistics University of Amsterdam Spuistraat 134 NL-1012 VB Amsterdam rens@alf.leLuva.nl Abstract ... estimate a probability as accurately as we want by choosing Nas large as we want, since according to the Strong Law of Large Numbers the estimated probability converges to the actual probability....

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... the Bradford assay with BSA as thestandard [28].Phytase activity assayPhytase activity was determined by measuring the amountof phosphate released from InsP6using a modiﬁed ferroussulfate ... neutral and alkaline pH; PhyH-DII was less sta-ble under the same conditions (Fig. 2D).PhyH was basically stable at 35 °C, and retained60% of the initial activity at 45 °C for 90 min whenassayed ... expressionwas induced by addition of IPTG (1 mm) and Ca2+(1 mm)for 20 h at 20 °C. Culture supernatants and cell pelletswere assayed for phytase activity and separated by SDS ⁄PAGE.Puriﬁcation was...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... Natl Acad Sci USA 99,11778–11783.9 Matsuda M, Nagahama Y, Shinomiya A, Sato T,Matsuda C, Kobayashi T, Morrey CE, Shibata N,Asakawa S, Shimizu N et al. (2002) DMY is a Y-spe-ciﬁc DM-domain ... sex-reversalmutants in the medaka, Oryzias latipes. Genetics 173,2083–2090.12 Otake H, Masuyama H, Mashima Y, Shinomiya A, Myosho T, Nagahama Y, Matsuda M, Hamaguchi S &Sakaizumi M (2009) ... Suzuki A, Kobayashi T, Paul-Prasanth B, Lau EL, HamaguchiS, Sakaizumi M & Nagahama Y (2007) DMY geneinduces male development in genetically female (XX)medaka ﬁsh. Proc Natl Acad Sci USA 104,...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... plasmid containing human a- synuclein cDNA (a- syn). As a control, we usedSH-SY5Y cells stably transfected with the plasmidcontaining b-galactosidase cDNA (b-gal). Western blotanalysis revealed ... kinase, 60Sacidic ribosomal protein P2 (RPLP2), eukaryoticinitiation factor 5A (eIF 5A) , parathymosin, L7 ⁄L12,annexin A2 , annexin A5 , aldolase A, fascin 1 andperoxyredoxin 1] displayed quantitative ... (Bruker-Daltonik, Bremen,Germany). Capillary voltage was 1.5–2 kV and a dry gasﬂow rate of 10 LÆmin)1was used with a temperature of230 °C. The scan range was 300–1800 m ⁄ z. The tandemmass spectra...

Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

... predictions, we have to await the structuralanalysis of a real enzymeÆreactivase complex.Experimental proceduresMaterialsCrystalline AdoCbl was a gift from Eisai (Tokyo, Japan).CN-Cbl was obtained ... K, Hieda N, Yamanishi M, Shibata N &Toraya T (2005) Crystallization and preliminary X-rayanalysis of molecular chaperone-like diol dehydratase-reactivating factor in ADP-bound and nucleotide-freeforms. ... the reactivase eluted separately at theirrespective retention times. In contrast, a peak of the freereactivase decreased and a new peak appeared in thepresence of ADP. The latter peak was eluted...

Tài liệu Báo cáo khoa học: Truyện ngắn phong tục với góc nhìn văn hóa pptx

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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... )oocytes display ultrastructuralabnormalities (Golgi apparatus, cortical actin cytoskele-ton, cytoplasmic aggregates), as well as mitochondrialdysfunction, in conjunction with markedly increasedproduction ... Eiberg H,Andres L, Jiang H, Zheng G, Qian M et al. (2002)Mutant DNA-binding domain of HSF4 is associatedwith autosomal dominant lamellar and Marner cata-ract. Nat Genet 31, 276–278.98 Smaoui ... rerio) heatshock factor 2 (HSF2) and similar patterns of HSF2and HSF1 mRNA expression in brain tissues. Biochi-mie 88, 1983–1988.121 Kawazoe Y, Tanabe M, Sasai N, Nagata K & Nakai A (1999)...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... decarboxylation catalysed by a- amino-b-carboxymuconate-e-semialdehyde decarbox-ylase. J Am Chem Soc 129, 9278–9279.20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H & ... action ofACMS decarboxylase (ACMSD, also known as picoli-nate carboxylase; EC 4.1.1.45) into a- aminomuconicKeywordscerebral malaria; kynurenine pathway;metal-dependent amidohydrolase; NADbiosynthesis; ... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization ofACMSD from Pseudomonas ﬂuorescens reveals a penta-coordinate mononuclear metallocofactor. J Am...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... molecular basis of enzyme thermosta-bility. J Bacteriol 185, 4038–4049.20 Nanba H, Yajima K, Takano M, Yamada Y, IkenakaY & Takahashi S (1997) Process for producing d-N-car-bamoyl -a- amino acid. ... molecular masswas also estimated by SDS–PAGE.Characterization and comparative analyses ofHYDJsand HYDBpThe optimal temperature for activity of HYDJswith d-p-HPH as substrate was determined ... DNAsequences ﬂanking the Jannaschia sp. CCS1 HYDJsrevealed an ORF encoding a putative allantoate amido-hydrolase, which is part of the urate catabolic pathwayin many organisms [8]. In fact,...

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