Tài liệu Báo cáo khoa học: Bacterial-induced hepoxilin A3 secretion as a pro-inflammatory mediator pptx

... the bifunctional enzymes chorismate mutase–prephenate dehydratase and chorismate mutase–prephenate dehydratase were deleted. Monofunctional versions of the dehydratase and the dehydrogenase are provided ... interactions may be an easy way to increase enzyme stability. This hexameric variant, however, suffered a 200-fold decrease in catalytic efficiency. In contrast, the unstable monomeric va...

Ngày tải lên: 19/02/2014, 12:20

... (ATIS) spoken language corpus. Preliminary experiments yield 96% test set parsing accuracy. 1 Motivation As soon as a formal grammar characterizes a non- trivial part of a natural language, ... Using an Annotated Corpus as a Stochastic Grammar Rens Bod Department of Computational Linguistics University of Amsterdam Spuistraat 134 NL-1012 VB Amsterdam rens@alf.leLuva.n...

Ngày tải lên: 22/02/2014, 10:20

... the Bradford assay with BSA as the standard [28]. Phytase activity assay Phytase activity was determined by measuring the amount of phosphate released from InsP 6 using a modified ferrous sulfate ... neutral and alkaline pH; PhyH-DII was less sta- ble under the same conditions (Fig. 2D). PhyH was basically stable at 35 °C, and retained 60% of the initial activity at 45 °C for 90 min when...

Ngày tải lên: 14/02/2014, 15:20

... Natl Acad Sci USA 99, 11778–11783. 9 Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, Kobayashi T, Morrey CE, Shibata N, Asakawa S, Shimizu N et al. (2002) DMY is a Y-spe- cific DM-domain ... sex-reversal mutants in the medaka, Oryzias latipes. Genetics 173, 2083–2090. 12 Otake H, Masuyama H, Mashima Y, Shinomiya A, Myosho T, Nagahama Y, Matsuda M, Hamaguchi S & Sakaizumi M (200...

Ngày tải lên: 14/02/2014, 19:20

... plasmid containing human a- synuclein cDNA (a- syn). As a control, we used SH-SY5Y cells stably transfected with the plasmid containing b-galactosidase cDNA (b-gal). Western blot analysis revealed ... kinase, 60S acidic ribosomal protein P2 (RPLP2), eukaryotic initiation factor 5A (eIF 5A) , parathymosin, L7 ⁄L12, annexin A2 , annexin A5 , aldolase A, fascin 1 and peroxyredoxin 1] di...

Ngày tải lên: 15/02/2014, 01:20

... predictions, we have to await the structural analysis of a real enzymeÆreactivase complex. Experimental procedures Materials Crystalline AdoCbl was a gift from Eisai (Tokyo, Japan). CN-Cbl was obtained ... K, Hieda N, Yamanishi M, Shibata N & Toraya T (2005) Crystallization and preliminary X-ray analysis of molecular chaperone-like diol dehydratase- reactivating factor in ADP-bound an...

Ngày tải lên: 15/02/2014, 01:20

...

Ngày tải lên: 18/02/2014, 03:20

... ) oocytes display ultrastructural abnormalities (Golgi apparatus, cortical actin cytoskele- ton, cytoplasmic aggregates), as well as mitochondrial dysfunction, in conjunction with markedly increased production ... Eiberg H, Andres L, Jiang H, Zheng G, Qian M et al. (2002) Mutant DNA-binding domain of HSF4 is associated with autosomal dominant lamellar and Marner cata- ract. Nat Genet 31,...

Ngày tải lên: 18/02/2014, 04:20

... decarboxylation catalysed by a- amino-b-carboxymuconate-e-semialdehyde decarbox- ylase. J Am Chem Soc 129, 9278–9279. 20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H & ... action of ACMS decarboxylase (ACMSD, also known as picoli- nate carboxylase; EC 4.1.1.45) into a- aminomuconic Keywords cerebral malaria; kynurenine pathway; metal-dependent amidohydrolase;...

Ngày tải lên: 18/02/2014, 06:20

... molecular basis of enzyme thermosta- bility. J Bacteriol 185, 4038–4049. 20 Nanba H, Yajima K, Takano M, Yamada Y, Ikenaka Y & Takahashi S (1997) Process for producing d-N-car- bamoyl -a- amino acid. ... molecular mass was also estimated by SDS–PAGE. Characterization and comparative analyses of HYD Js and HYD Bp The optimal temperature for activity of HYD Js with d-p- HPH as substrate w...

Ngày tải lên: 18/02/2014, 08:20