Tài liệu Báo cáo khoa học: Sensor of phospholipids inStreptomycesphospholipase D pdf

... structure of TH-2PLD is sho- wn using the Swiss-PDB viewer and is based on the crystal structure of PMFPLD. The identified key residues are indicated in red. The N-terminal and C-terminal HKD motifs ... 000, 53 500, 36 100 and 29 500 Da). The samples were loaded on a 10% acrylamide gel. The arrowhead indicates the position of the purified PLDs. Sensor of phospholipids in PLD Y. Uesu...

Ngày tải lên: 19/02/2014, 02:20

... were of reagent grade. SR was purified and washed with DOC as described previously [13,14]. The purified SR and DOC-washed SR were stored in liquid nitrogen. The protein concentration in SR was determined ... and were adsorbed onto the mica surface as lipid bilayer mem- branes with a thickness of 5.1 ± 0.6 nm [mean ± standard deviation (SD), N = 23]. These DOC-treated SR membranes immobiliz...

Ngày tải lên: 14/02/2014, 14:20

... 5,5¢-dithio-bis(2-nitrobenzoic acid) and NADPH at 340 nm. GSSG was determined by the same method in the presence of 2-vinylpyridine and reduced GSH was calculated from the difference between total glu- tathione and GSSG ... GdCl 3 attenuated the imbalanced vascular stress gene expression induced by sepsis [17]. In the present study, the depletion of KCs was confirmed by dramatically reduce...

Ngày tải lên: 14/02/2014, 18:20

... code: 1N9J); (B) stefin B monomer (Protein Data Bank code: 1stf) and domain-swapped dimer (Protein Data Bank code: 2oct); and (C) proposed mechanism of the building up of amyloid fibrils obtained ... studies, cellular and animal models, and clinical studies. In addition to providing a basic understanding of the pro- cesses of protein folding and aggregation, such data help towards trans...

Ngày tải lên: 14/02/2014, 18:20

... intensity indeed denoted the formation of higher molecular mass aggregates, SDS ⁄ PAGE and immuno- blotting were carried out according to the procedure described in Materials and methods. a-Synuclein ... patients of idiopathic Parkinson’s disease and develop the same therapy-related complications. Post- mortem analysis of brains of patients with MPTP- induced Parkinson’s disease has d...

Ngày tải lên: 14/02/2014, 19:20

... and hydrolysis have been estimated sequentially from the same mitochondria: first mitochondria were energized, a small amount of uncoupler was added, then ADP was added, and ATP efflux was recorded; ... recorded; 150 s later, 1 lm of SF 6847 was added, and ATP influx was recorded; after 150 s, alamethi- cin was added, and ATP hydrolysis by the F 0 F 1 -ATP syn- thase was recorded). F min and...

Ngày tải lên: 14/02/2014, 19:20

... of individuals, mirroring a highly balanced degree of co-evolution of H. pylori and humans. Studies of host–bacterial interactions and vir- ulence factors CagA and the T4SS have provided us with ... as useful in vivo models to study H. pylori-induced pathology. However, each ani- mal model has distinct advantages and disadvantages, and therefore only can be considered as complemen- ta...

Ngày tải lên: 14/02/2014, 19:20

... oleate, inhibited cell proliferation and induced cell death; (b) stearate-induced cell death had the characteristics of endoplasmic reticulum stress-mediated and mitochondrial-mediated apoptosis; ... harvested, and lipids were extracted twice with CHCl 3 ⁄ MeOH ⁄ ddH 2 O (1 : 1 : 0.9). Lipid extracts were dried under a nitrogen stream, redis- solved in chloroform ⁄ methanol (2 : 1), and sep...

Ngày tải lên: 14/02/2014, 22:20

... increasing DP may be a common feature of glucansucrase. Additional studies are required to elucidate the dex- tran-dependent synthesis of glucan. As the affinity of the GBd of GTF-I for dextran is ... procedures. Filled and open circles indi- cate the GTF activity of GSGB and GS, respectively. A B Fig. 3. Saccharide production kinetics during the dextran-indepen- dent GTF-I reaction....

Ngày tải lên: 14/02/2014, 22:20

... CAD ⁄ ICAD DFF is composed of two subunits, a 40 kDa caspase- activated DNase (CAD) ⁄ DFF40 and a 45 kDa inhibi- tor of CAD (ICAD ⁄ DFF45), the complex of which is an inactive form. During apoptosis, ... MAPK 185 183 Degradation 125 p53 Bax Akt 473 Caspase-3 ERK1 70 87 69 Apaf-1 Apoptosome Caspase-9 Cleaved caspase-3 Endo GAIF Dimerization PARP DNA fragmentation Nucleus Cytoplasm CAD IC...

Ngày tải lên: 14/02/2014, 22:20