Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf

... H + - ATPase inhibitor bafilomycin and the cathepsin D inhibitor pepstatin A partially inhibited, both in vivo and in vitro, the cAMP response to cholera toxin. This cathepsin D- dependent action of cholera ... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of bot...

Ngày tải lên: 19/02/2014, 02:20

... T7 and ADC2, and (b) T7 and RIB – under the conditions described in the Experimental procedures. PCR products were analysed by electrophoresis on agarose gels containing ethi- dium bromide and ... radioactive bands obtained in the corresponding Southern blot assay (Fig. 3B) could only be explained by the integration, into the parasite genome, of ‡ 2 units of the plasmid pADC-8...

Ngày tải lên: 19/02/2014, 07:20

... lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, we employed GdCl 3 to inactivate KCs based on the findings of other investigators ... 5,5¢-dithio-bis(2-nitrobenzoic acid) and NADPH at 340 nm. GSSG was determined by the same method in the presence of 2-vinylpyridine and reduced GSH was calculated from t...

Ngày tải lên: 14/02/2014, 18:20

... manner dependent on integrin b 1 . Injected CagA can then induce cascades as depicted in the panels below. (A) Highlighting a multitude of known T4SS-dependent but CagA-independent pathways involved ... labelling by antibodies made specifically against this subdomain [10]; however, yeast-two hybrid screens and other in vitro binding studies indicated that the very carboxy-terminus inte...

Ngày tải lên: 14/02/2014, 19:20

... acting as a ligand-inducible transcription factor and has been implicated in the control of keratinocyte survival. In the mouse keratinocyte cell line SP1, serum starvation induced cell death and ... rap- idly elevated in the epidermis at the wound edges; dele- tion of a single PPARb allele results in delayed wound healing [10]. Furthermore, in ammation-induced apo- ptosis wa...

Ngày tải lên: 18/02/2014, 18:20

... treated with EGF and ADR as described in (A), harvested at the indicated times after the addition of ADR, and used for immunoblot analysis of pro-caspase 9 and caspase 9. (C) Cells were treated with ... the transactivation domain of c-Jun (amino acids 1–122), but retains the DNA binding and leucine-zipper region of c-Jun, it should function as a dominant-negative mutant of...

Ngày tải lên: 19/02/2014, 06:20

... charged amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a single interaction would not ... leads to a more detailed understanding of the role of K22 and R120 with respect to fosfomycin binding as well as the ligand-induced conformational switch. Experimental proc...

Ngày tải lên: 19/02/2014, 13:20

... large in uence on conformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. ... calculated using c utoffs of 3.6 A ˚ and 4.1 A ˚ [19]. All hydrophobic c ontacts and h ydrogen bonds that are involved in the inhibitor OE binding ar e summarized in T...

Ngày tải lên: 19/02/2014, 16:20

... amino acids of the extracellular domain and devoid of any tag was not functional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag was found constitutively ... held together by disulfide bonds. Moreover, part of the a-subunit of the receptor is shed from thyroid and transfected L cells. To understand the role of cleavage and...

Ngày tải lên: 21/02/2014, 00:20

... procedures. Filled and open circles indi- cate the GTF activity of GSGB and GS, respectively. A B Fig. 3. Saccharide production kinetics during the dextran-indepen- dent GTF-I reaction. (A) and ... there- fore, to understand the mechanism underlying the dextran-dependent GTF-I reaction, the dynamics of binding of GTF-I to dextran should be investigated with the use of single-mol...

Ngày tải lên: 14/02/2014, 22:20