Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... H+-ATPase inhibitor baﬁlomycin and the cathepsin D inhibitor pepstatin Apartially inhibited, both in vivo and in vitro, the cAMP response to cholera toxin. This cathepsin D- dependent action of cholera ... action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits wasobserved, coincident ... cathepsin D FEBS Journal 274 (2007) 2614–2629 ª 2007 The Authors Journal compilation ª 2007 FEBS 2629 Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity Tatiana...

Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... T7 and ADC2, and (b) T7 and RIB – underthe conditions described in the Experimental procedures. PCR products were analysed by electrophoresis on agarose gels containing ethi-dium bromide and ... radioactive bands obtained in the corresponding Southern blot assay (Fig. 3B) couldonly be explained by the integration, into the parasitegenome, of ‡ 2 units of the plasmid pADC-8 arranged in a head-to-tail ... spermidine. On the otherhand, all our attempts to detect ODC or ADC enzy-matic activities in various strains of wild-type T. cruziepimastigotes, by adding radioactive ornithine orarginine to intact...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... lead-ing to hepatotoxicity in liver injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, we employed GdCl3to inactivate KCs basedon the ﬁndings of other investigators ... 5,5¢-dithio-bis(2-nitrobenzoic acid) and NADPH at 340 nm. GSSG was determined by thesame method in the presence of 2-vinylpyridine and reducedGSH was calculated from the difference between total glu-tathione and GSSG ... prevented lipidperoxidation and a decrease in hepatic GSH ⁄ GSSGratio during sepsis. Thus, the results of the presentstudy suggest that ROS produced by KCs mediate thesepsis-induced decrease in...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... mannerdependent on integrin b1. Injected CagA can then induce cascades as depicted in the panels below. (A) Highlighting a multitude of knownT4SS-dependent but CagA-independent pathways involved ... labelling by antibodies made speciﬁcallyagainst this subdomain [10]; however, yeast-two hybridscreens and other in vitro binding studies indicated thatthe very carboxy-terminus interacted with integrin ... number of bacteria gain access to integrins and inject CagA. Thebasal injection model of CagA can also explain whyH. pylori does not cause more gastric damage in infected individuals and may...

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... acting as a ligand-inducible transcriptionfactor and has been implicated in the control of keratinocyte survival. In the mouse keratinocyte cell line SP1, serum starvation induced cell death and ... rap-idly elevated in the epidermis at the wound edges; dele-tion of a single PPARb allele results in delayed woundhealing [10]. Furthermore, in ammation-induced apo-ptosis was found to be ... signiﬁcantly increasedthe TEWL rate and resulted in skin barrier disruption(Fig. 1A). PPARb mRNA expression was determined in keratinocytes isolated from the epidermal layer of these skins, and was...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... treated with EGF and ADR asdescribed in (A), harvested at the indicated times after the addition of ADR, and used for immunoblot analysis of pro-caspase 9 and caspase 9. (C) Cells were treated with ... thetransactivation domain of c-Jun (amino acids 1–122),but retains the DNA binding and leucine-zipper region of c-Jun, it should function as a dominant-negativemutant of c-Jun to block wild-type c-Jun binding ... also examinedthe effect of PD98059 on this binding activity and, asexpected, observed a decrease in AP1 DNA binding.Supershift analysis using antibodies speciﬁc for allknown Fos and Jun family...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... leads to a more detailed understanding of the role of K22 and R120 with respect to fosfomycin binding as wellas the ligand-induced conformational switch.Experimental proceduresChemicals and ... Temperature dependence of DH (circles, solid lines), DG(squares, dotted line) and –TDS (triangles, dashed lines) for UDPNAGbinding to the K22VR120K (A), K22V (B) and R120K mutant proteins(C). Thermodynamic...

Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S conﬁguration of isostere in binding properties of HIV-1 protease inhibitors docx

... large in uence onconformation of inhibitor main chain. Thus, differentinhibitors differ not only in t he binding afﬁnity, bu t a lso in the degree o f freedom of the inhibitor inside the bindingtunnel. ... calculated using c utoffs of 3.6 A˚ and 4.1 A˚[19]. Allhydrophobic c ontacts and h ydrogen bonds that are involved in the inhibitor OE binding ar e summarized in Table 3.Conformation of isostere. ... [5,7–9].Our recent studies of the hydroxyethylamine inhibitorcomplexes [ 7–9] revealed that the binding tunnel of p rotease,abundant in hydrogen bond donors and acceptors, canbind the inhibitors i...

Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafﬁcking pdf

... amino acids of theextracellular domain and devoid of any tag was notfunctional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag wasfound constitutively ... held together by disulﬁde bonds. Moreover, part of the a-subunit of the receptor is shed from thyroid and transfected L cells. To understand the role of cleavage and shedding, we constructed deletion ... arguefor a diﬀerent conformation between the receptor activatedby cleavage and shedding on the one hand, and the receptoractivated by the ligand on the other hand. Cleavage and shedding of a receptor...

Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

... procedures. Filled and open circles indi-cate the GTF activity of GSGB and GS, respectively.ABFig. 3. Saccharide production kinetics during the dextran-indepen-dent GTF-I reaction. (A) and ... there-fore, to understand the mechanism underlying thedextran-dependent GTF-I reaction, the dynamics of binding of GTF-I to dextran should be investigated withthe use of single-molecule measurements, ... properties and the process of insoluble glucan synthesis remains unknown.To address these issues, we analyzed the process of dextran-independent insoluble glucan formation and examined the enzyme kinetic...

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