Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis docx

... model of the a subunit of the terminal dioxygenase (AtdA3), was used to probe the molecular determinants of AtdA substrate specificity. The V205A mutation widened the substrate specificity of aniline ... and applied saturation mutagenesis to these residues to probe the molecular determinants of the activity and specificity of the enzyme. We ha...

Ngày tải lên: 19/02/2014, 02:20

... is located in the vicinity of the exocyclic substituent of the substrate. Although mutagenesis of Phe150 into other residues caused nearly complete activity loss, replacement of Phe150 by Tyr retained ... understanding of the roles of each of these residues will enable manipu- lation of these SGLs by rational design or molecular evolution methods to obtain th...

Ngày tải lên: 18/02/2014, 08:20

... content of the translations, the translation quality, and the user-friendliness. The list of criteria for self evaluation consisted of technical, linguistic and ergonomic issues. As part of the ... obtained the triples in ta- ble 1. The first number in a triple is the percentage of positive counts in the high frequency class, the second number is the percent...

Ngày tải lên: 22/02/2014, 03:20

... resonances without the concomitant appearance of other signals in other parts of the spectrum. This result could be a consequence of the paramagnetic properties of Fe(II), or it might arise from the presence of ... Fe 3+ Addition of Fe 2+ induced the displacement of several resonances in the [ 1 H, 15 N]-HSQC NMR spectrum of CyaY, but the most striking consequenc...

Ngày tải lên: 18/02/2014, 16:20

... words. Therefore, missing words can be used to prune the hypotheses that are also highly related to the missing words. Consider the hypotheses of latent vectors in ta- ble 1 for bank#n#1. Assume there ... WMF on the definitions corpus, the similarity of two senses s j and s k can be computed by the inner product of Q ·,j and Q ·,k . 2.3 A Nuanced Sense Similarity: wmfvec We...

Ngày tải lên: 19/02/2014, 19:20

... spanned either the second quarter of the TC sequence GQLA…TAAM(103–198) or the C-ter- minal peptide LEPA…LVSW(316–427). Proteolytic fragments of TC and the synthetic peptides were used to further ... [14] despite the absence of S-S bonds and the low number of conserved residues in this part of the sequence. Only later did the large N-terminal unit become involved in...

Ngày tải lên: 20/02/2014, 01:20

... pres- ence of H 2 O 2 alone, a non-physiological condition. We believe that the absence of direct evidence of the radical in the presence of cosubstrates limits the validity of extrapo- lating the model ... oxidize them [19]. On the other hand, this fact does not represent proof of the presence of one or two oxidizing equivalents on the decay product of Cpd I....

Ngày tải lên: 20/02/2014, 02:21

... cost by # of sentences versus # of words, consider Figures 5 and 6. They both show the same three selection methods but Figure 5 measures the x-axis by # of sentences and Figure 6 measures by # of ... labeling. In Proceedings of the Joint Conference of the 47th An- nual Meeting of the ACL and the 4th International Joint Conference on Natural Language Processing...

Ngày tải lên: 20/02/2014, 04:20

... best IM:Type full time IM:Title the godfather Other the, in, that Other the, in, that Other the, in, that Table 3: Label sets and their respective query segment examples for the intent class Movie, Job ... goal of this work is to identify the semantic labels of IMs, i.e., the attribute names they implicitly refer to. We use A c to denote the set of IM semantic labels for t...

Ngày tải lên: 20/02/2014, 04:20

... polarity, hits w,pos is the number of hits returned by a com- mercial search engine when the search query is the given word and the disjunction of all positive seed words. hits pos is the number of hits when ... engine. The number of hits for positive seeds, negative seeds, positives seeds near the given word, and negative seeds near the given word are used to estima...

Ngày tải lên: 20/02/2014, 05:20