Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al. (2003) Genome sequence of Bacillus cereus and comparative analysis with ... 276–280. 7 Handa N, Terada T, Kamewari Y, Hamana H, Tame JRH, Park S-Y, Kinoshita K, Ota M, Nakamura H, Kuramitsu S et al. (2003) Crystal structure of the con- served prote...

Ngày tải lên: 19/02/2014, 00:20

... bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table 3). The angles around the metal cofactor sug- gested ... Uegaki K, Hagihara Y, Ataka M & Ishikawa K (2005) Crystallization and preliminary X-ray diffraction analysis of thioredoxin peroxidase from the aerobic hy...

Ngày tải lên: 14/02/2014, 22:20

... on surface analysis, we propose a rigid domain association for the N-terminal SEA domain with the back site of the proteinase domain. Abbreviations HAI, hepatocyte growth factor activator inhibitor; ... regions of these proteinases medi- ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of...

Ngày tải lên: 19/02/2014, 00:20

... weak homodimer, as observed for MaL, that may determine the properties of these asco-laccases at high protein concentrations. Database Structural data are available in the Protein Data Bank database ... ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases Juha P. Kallio 1 , Chiara Gasparetti 2 , Martina Andberg 2 , Harry Boer 2 , Anu Ko...

Ngày tải lên: 14/02/2014, 18:20

... by the importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma- tional rearrangement that exposes the NLS in an extended conformation. Database Structural data are available ... Koochek A, Ryscavage A, Bhat K, Tanaka T, Oshima A, Fitzgerald P & Yuspa SH (2007) Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of...

Ngày tải lên: 14/02/2014, 19:20

... phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two domains [4,5]. HAPs can initi- ate ... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A...

Ngày tải lên: 16/02/2014, 09:20

... Tritirachium album Limber and thermi- tase from the thermophilic eubacterium Thermoactino- mycetes vulgaris. Results The crystal structure of the Vibrio proteinase The obtained Vibrio proteinase crystals ... it is part of a molecular strategy of cold adaptation. Because of the ordering of water structure at low temperature (i.e. below approximately the tem- per...

Ngày tải lên: 19/02/2014, 16:20

... oligonucleotides: a sense primer 5¢-CAACAAATTTG CATATGACTATT AAAG-3¢ containing an NdeI site (underlined) next to the start codon of the T. brucei gGAPDH gene; an anti- sense primer 5¢-CAGCCAAGCG CCTAGGGAGCGAGA AC-3¢, ... Mikaelian & Sergeant [33] and using the Vent DNA polymerase. The T. brucei gGAPDH Thr196 ACA codon was changed into the Ala codon GCA, and the Thr225 cod...

Ngày tải lên: 20/02/2014, 02:21

... indole-3-acetic acid (IAA), which is synthe- sized by plants [1,2] and plant-associated bacteria [3,4]. Several pathways for the synthesis of IAA in these organisms have been described, and most of them ... bonds with protein atoms. The final R-values and other refinement statistics are given in Table 1. The X-ray data and the atomic coordinates have been deposited at the Prote...

Ngày tải lên: 20/02/2014, 11:20

... TtAKb. Abbreviations AK, aspartate kinase; BsAKII, aspartate kinase II from Bacillus subtilis; CgAK, aspartate kinase from Corynebacterium glutamicum; CgAKb, regulatory subunit of aspartate kinase from Corynebacterium ... Tomita T, Kuzuyama T & Nishiyama M (2007) Purification, crystallization and preliminary X-ray analysis of regulatory subunit of aspartate kinase from Ther...

Ngày tải lên: 18/02/2014, 08:20