Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

... intracellular distribution of K-Ras in CHO-K1 cells is modulated by electrical properties of plasma membrane and endomembranes, which are relevant to K-Ras signaling. Results Membrane association and subcellular distribution ... regulate subcellular distribution of Ras isoforms. Effect of ATP depletion on subcellular distribution of K-Ras To characteriz...

Ngày tải lên: 19/02/2014, 00:20

... rotation of the bTD module at the main chain of D606 to dock it against the calf-2 domain with its C-terminus in prox- imity of the N-terminus of the b 3 ‘open’ TM helix. Overall evaluation of the ... structure of the integrin aIIbb3 transmembrane complex explains integrin transmembrane signaling. EMBO J 28, 1351–1361. 15 Yang J, Ma YQ, Page RC, Misra S, Plow EF & Qin J (2009)...

Ngày tải lên: 18/02/2014, 04:20

... based on the level of the fluorescence signal observed and the concentration of primary antibodies used. However, when subcellular localization of cystatin F was compared with that of the cathepsins, ... localization of cystatin F, its restricted and readily regulated expres- sion [18], selective and not too potent inhibition of cathepsins are all in favor of the active role o...

Ngày tải lên: 19/02/2014, 17:20

... thank Prof. Steve C. Fry, Institute of Cell and Molecular Biology, Univer- sity of Edinburgh, Edinburgh, UK, and Dr Albert Schulte, Ruhr University of Bochum, Germany, for critical reading of the ... quantities of protein util- izing the principle of protein-dye binding. Anal Biochem 72, 248–254. 33 Laemmli UK (1970) Cleavage of structural proteins dur- ing the assembly of the...

Ngày tải lên: 20/02/2014, 01:20

... one of a few major Myb-interacting proteins. The moderate basal level of sumoylation was greatly enhanced by cotransfection of PIASy, an E3 ligase for SUMO-1. The functional consequence of abolishing ... to the importance of the c-Myb–Ubc9 interaction. We conclude that SUMO-1 conjugation of c-Myb is not only a phenomenon induced under favourable conditions of overexpression of...

Ngày tải lên: 21/02/2014, 00:20

... reticulum membrane marker, dilution 1 : 2500) (Abcam) sera were used to identify the subcellular localization of RPE65c and to verify the endoplasmic reticulum membrane preparation. The distribution of ... absence of a metal chelator, RPE65c cat- alyzed the production of 11cROL and 13cROL from atRE (Fig. 4A). However, in the presence of 1 m M of the metal chelator, bipyrid...

Ngày tải lên: 14/02/2014, 14:20

... second largest family of cyto- plasmic tyrosine kinases. In 1993, loss -of- function mutations in the BTK gene were reported as the cause of X-linked agammaglobulinemia. Of all the existing 90 ... various domains of BTK. Mutations affecting the R28 residue (marked in dark blue in Fig. 1) will result in the redistribution of electrostatic charges that are Fig. 1. Structure of PH, S...

Ngày tải lên: 14/02/2014, 18:20

... entry of fatty acid into mitochon- dria. Thus, the steady-state level of malonyl-CoA in these tissues is determined by the relative activities of ACC and malonyl-CoA decarboxylase. Role of the ... aspects of the affects of fructose on food intake, these findings lend further support to the mech- anism by which malonyl-CoA participates in the regu- lation of feeding behavior. Role...

Ngày tải lên: 14/02/2014, 22:20

... O & Haguenauer-Tsapis R (2000) Ubiquitination and endocytosis of plasma membrane proteins: role of Nedd4 ⁄ Rsp5p family of ubiquitin- protein ligases. J Membr Biol 176, 1–17. 2 Fang D, Elly ... downstream activation of a family of cytosolic cysteine proteases, caspases, which are required for many of the morphological changes that occur during apoptosis. The mitochondrial...

Ngày tải lên: 16/02/2014, 09:20

... of the other rhodamine dyes were somewhat higher, but still < 3.5 times the amounts adsorbed to the cell surface. Because the amount of intracellular membranes exceeds that of the plasma membrane, ... intracellular membranes because they are hydrophobic and negatively charged. A comparison of rhodamine uptake, in the presence and the absence of uncouplers, allows the estimatio...

Ngày tải lên: 18/02/2014, 13:20