Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... with the levels of DNA methylation, and thus the effect of the abso- lute configurations and conformations of the adducts on DNA methylation was not evaluated. More recently, the effect of stereochemically ... (2007) 2121–2134 ª 2007 The Authors Journal compilation ª 2007 FEBS The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects...

Ngày tải lên: 19/02/2014, 00:20

... mediated by the tracheal system, a network of tubules that open to the atmosphere on the cuticle and radiate to all parts of the body. O 2 is delivered through trachea and trache- oles in the gaseous ... indication of the presence of hemocyanin (data not shown). The notion of the absence of hemo- cyanins from Holometabola is corroborated by the fact that...

Ngày tải lên: 18/02/2014, 08:20

... (see below). The effect of the additives on aggregation of the target protein (in the absence and presence of aB-crystallin) was assessed at the end of each assay by calculating the percentage ... aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde- pendent and different from that of the chaperone). The st...

Ngày tải lên: 18/02/2014, 16:20

... times of digestion, more than 50% of the sample was converted into the frac- tion S. Breaking the disulfate bond in the middle of the crosslinker produced only mononucleosomes and the band S. DNA ... rest, even by the zero-length crosslinker, perhaps via linker histones H1 and H5. Indeed, there is evidence that the tails of these histones follow the path of...

Ngày tải lên: 18/02/2014, 18:20

... prevented by blocking transport from the ER to the Golgi. BFA had no effect on the relative lack of the 130 kDa isoform of the K650M mutant. Endo H digestion of the immunoprecipitated wild-type and ... and located in different domains of the recep- tor (Fig. S1) was created by site-directed mutagenesis of the full-length human FGFR3 cDNA and subclon- ing in...

Ngày tải lên: 19/02/2014, 00:20

... electrostatic field of different Fig. 5. Plot of the EIE values as function of rotation (r) and inclination (i) angles of RNases. The hatched axis at the bottom and right of the plots show schemes of some ... DMPG membranes, as evidenced by a decrease of the amplitude of their thermotropic transition. Only RNase A and RNase AA show no effects on the thermotr...

Ngày tải lên: 19/02/2014, 06:20

... from the deleterious effects of DNA injury a net- work of genome care-taking mechanisms has evolved of which a set of DNA repair systems constitute a key component. The clinical impact of the DNA ... undergoes fusion with the membrane of intra-endosomal vesicles contained within MVBs, thereby releasing the nucleocapsid into the lumen of these vesi- cles. Then,...

Ngày tải lên: 19/02/2014, 07:20

... Electron density of RTA R213D in the vicinity of residue 213. The backbone and sidechains of the R213D substitution are shown as stereo images in thick ball and stick and the position of the Arg213 ... by the addition of 100 lLof2· Kirby buffer [25] and 80 lLofH 2 O, and rRNA was obtained by precipitation after two phenol– chloroform extractions. Four micro...

Ngày tải lên: 19/02/2014, 12:20

... the lysozyme of the bivalve Tapes japonica [3], with the so-called chlamysin of Chlamys islandica [4,5], and also with a hypothetical secreted protein of the nematode Caenorhabditis elegans and with ... between this plot and the corresponding part of the HCA plot of the Asterias lysozyme. The same overlap is obtained when comparing the human lysozyme and/ or...

Ngày tải lên: 19/02/2014, 12:20

... revealed the existence of two additional subunits (bringing the total to 46), and one of these, the ESSS protein, is also encoded by an X-linked g ene in humans and mouse [36]. Most of these subunits ... the level of a complex of 46 subunits is determined. The activities of complex II and the downstream complex III of the electron transport chain were measu...

Ngày tải lên: 19/02/2014, 16:20