Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx
Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx
... al. Active recombinant hepcidinFEBS Journal 275 (2008) 3793–3803 ª 2008 The Authors Journal compilation ª 2008 FEBS 3803 Production of biologically active forms of recombinant hepcidin, the iron-regulatory ... peptide hormone that acts as the central regulator of body iron metabolism. Hepcidin is synthesized under the form of a precursor, prohepcidin, which is processed to produce the biologically active ... oxidized and biologically active. Further-more, the need of denaturation–renaturation orreduction–oxidation steps (often present in other pro-tocols of hepcidin preparation, either recombinant [26–28]...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... considering the oxidation of the substrate byT. reesei TYR2. The closer to the hydroxyl group of phenol the amino group was, the slower was the oxida-tion of the substrate. For most of the substrates ... changes the redox potential of the phenolic substrates, makingthem more easily oxidized. Therefore, the pH profilereflects not only the optimal behavior of the enzyme,but also changes in the substrate. ... these states are alternated, has beenproposed [16]. The met state is the resting state of the enzyme, and in the absence of substrate about 85–90% of the enzyme is in this state [16]. Both the...
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Tài liệu Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx
... microscopystudy of the neuronal endings in the sinus gland todetermine the subcellular localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of enzyme(s) ... terminal in the sinus gland, as a result of late and progressiveisomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonaltract [39,41]. The immunohistochemical ... whereasseveral somas of the two types were dispersed in the X organ (Fig. 3A), most likely as a result of arte-factual displacement of the cell bodies during the prep-aration of the organ. In the sinus...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BHenzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range(pH ... andBHAb. The temperature dependence of the activities of the BHs was tested in the range 5–56 °C; the apparentoptimum temperatures of BHLmL2 were 25–30 °CTable 3. Masses and scores of tryptic ... cultures of L. macu-lans (twofold higher than that of camalexin). For thisreason, 3-phenylindole (0.2 mm) was used to induce the biosynthesis of BHs to facilitate their purification.Purification of...
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Tài liệu Báo cáo khoa học: Identification, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx
... of the CA D. melanogastersequences and, on the other hand, the otherD. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano-gaster-3) or to the ... wasobserved in the cytoplasm of the cells. The stainingappears to be specific of the probe sequence because the staining is clear with the complementary sequenceto RpCAbr, but not with the sense ... amplifications of RpCAbr and RpCAtr with 18Samplification. The number of cycles on the y-axis is the differencebetween the number of cycles required to amplify each transcriptand the number of cycles...
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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... interruption of the geometry of the recognition helix for either Antp or the TTF-1HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion of helix III ... inertia.In the context of RSDM, the detailed analysis of the three spectral densities J(0), J(xN) and J(0.87xH)allowed us to obtain a rather complete description of the dynamics of the TTF-1 ... hydrodynamicradius of 1.98 nm for the TTF-1 HD under the condi-tions of this study, i.e. very close to the mean radius of the NMR structure of the molecule (1.94 nm) [4]. The conclusions inferred...
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Tài liệu Báo cáo khoa học: ATP-dependent modulation and autophosphorylation of rapeseed 2-Cys peroxiredoxin docx
... bondyielding the reduced form of the sulfur atom at Cys175 (reaction 1). The subsequent oxidation transforms the sulfhydryl group into the sulfinic and sulfonic species (reaction 2). The reactivities of thesegroups ... autophosphoryla-tion of the Y166F mutant was similar to wild-type2-Cys Prx. Given the absence of another putative site,we approached the localization of the phosphoryl moi-ety by examining the mass spectra of ... Cys175–Gly178-).Because the size of the cavity (0.515 nm3) in silico eas-ily docks a molecule of ATP (0.33 nm3), it was impor-tant to know whether the functions of 2-Cys Prx weresensitive to insertion of the...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
... with other M-superfamily cono-toxins.Nevertheless, the cDNA structure of conomarphinwas similar to those of other conotoxin cDNAs. The cDNA-encoded precursor of conomarphin consisted of a conserved ... fromcleavage of the signal peptide and the propeptide,which happens in the maturation process for everyconotoxin [8], the removal of the two additional resi-dues Leu and Val at the C-terminus is rather ... 2008 The Authors Journal compilation ª 2008 FEBS 1977effect of the d-Phe on structure, was also studied. The unusual structure of conomarphin further demon-strates the high diversity of conotoxins.ResultsPurification...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... analyses of the predictedamino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro-teins. The ... to model the entire domain without it.Another significant finding concerns the nature of the acid residues within the active site of Rep245. The carboxylate triad of Rep245 including the D101, ... between the catalytic subunit of archaeal primases and the active site of the X family of polymerases [23]. Indeed, unlike the pRN1 prim–pol protein whose motif is DXE D, the Rep245 protein, the...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
... duringprotein synthesis [21,33], we investigated whether the production of the human recombinant isoforms mightbe enhanced by the presence of metal ions in the growth medium. We found that addition of 200 ... mark the boundary of the predicted substrate-binding pocket,reveals that some of them are conserved in the inac-tive isoform (i.e. Trp191 and Phe294). Even though the precise role of these active ... efficiency. The copy number of each variant in the unknown samples was determined from the calibrationcurves and data were normalized to the copy number of 18S RNA.Expression of ACMSD isoforms in...
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