Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx

... al. Active recombinant hepcidin FEBS Journal 275 (2008) 3793–3803 ª 2008 The Authors Journal compilation ª 2008 FEBS 3803 Production of biologically active forms of recombinant hepcidin, the iron-regulatory ... peptide hormone that acts as the central regulator of body iron metabolism. Hepcidin is synthesized under the form of a precursor, prohepcidin, whic...

Ngày tải lên: 18/02/2014, 18:20

... considering the oxidation of the substrate by T. reesei TYR2. The closer to the hydroxyl group of phenol the amino group was, the slower was the oxida- tion of the substrate. For most of the substrates ... changes the redox potential of the phenolic substrates, making them more easily oxidized. Therefore, the pH profile reflects not only the optimal behavior...

Ngày tải lên: 19/02/2014, 06:20

... microscopy study of the neuronal endings in the sinus gland to determine the subcellular localization of the different epimers. Taken together, these results allow a discus- sion of the existence of enzyme(s) ... terminal in the sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicl...

Ngày tải lên: 18/02/2014, 11:20

... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BH enzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range (pH ... and BHAb. The temperature dependence of the activities of the BHs was tested in the range 5–56 °C; the apparent optimum temperatures of BHLmL2 were 25–30 °...

Ngày tải lên: 18/02/2014, 14:20

... of the CA D. melanogaster sequences and, on the other hand, the other D. melanogaster sequences more closely related to the CAVI vertebrate isoforms (CAa, CAb and D. melano- gaster-3) or to the ... was observed in the cytoplasm of the cells. The staining appears to be specific of the probe sequence because the staining is clear with the complementary sequence to RpC...

Ngày tải lên: 18/02/2014, 16:20

... interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion of helix III ... inertia. In the context of RSDM, the detailed analysis of the three spectral densities J(0), J(x N ) and J(0.87x H ) allowed us to obtain a rather complete description...

Ngày tải lên: 18/02/2014, 16:20

... bond yielding the reduced form of the sulfur atom at Cys175 (reaction 1). The subsequent oxidation transforms the sulfhydryl group into the sulfinic and sulfonic species (reaction 2). The reactivities of these groups ... autophosphoryla- tion of the Y166F mutant was similar to wild-type 2-Cys Prx. Given the absence of another putative site, we approached the localizat...

Ngày tải lên: 18/02/2014, 17:20

... with other M-superfamily cono- toxins. Nevertheless, the cDNA structure of conomarphin was similar to those of other conotoxin cDNAs. The cDNA-encoded precursor of conomarphin consisted of a conserved ... from cleavage of the signal peptide and the propeptide, which happens in the maturation process for every conotoxin [8], the removal of the two additional resi- due...

Ngày tải lên: 18/02/2014, 17:20

... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro- teins. The ... to model the entire domain without it. Another significant finding concerns the nature of the acid residues within the active site of Rep245. The carboxyla...

Ngày tải lên: 18/02/2014, 18:20

... during protein synthesis [21,33], we investigated whether the production of the human recombinant isoforms might be enhanced by the presence of metal ions in the growth medium. We found that addition of 200 ... mark the boundary of the predicted substrate-binding pocket, reveals that some of them are conserved in the inac- tive isoform (i.e. Trp191 and Phe294). Eve...

Ngày tải lên: 19/02/2014, 02:20