Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx

... with a construct spanning 4.9 kb upstream of the transcriptional start site strongly indicate differ- ences in transcriptional regulation. The strong synergy generated by the combination of FGF-2 ... For other controls, pri- mary or secondary antibodies were omitted. Acknowledgements The authors are obliged to Professor Rolf Howlett for the preparation and donation of wound...

Ngày tải lên: 19/02/2014, 18:20

... compute the frequency of all the candidates identified in all snippets, and output the one with the highest frequency. 4 Experiments and Evaluation We extracted the Wikipedia titles of English and ... translations in another language. By retrieving and identifying such translation counterparts on the Web, we can cope with the OOV problem. Consider the tec...

Ngày tải lên: 19/02/2014, 19:20

... Dicer protein [35]. As expected, the band was shifted in the presence of the Dicer antibody but not the other antibodies. We also wished to confirm the location of any TRBP-containing bands, if these ... the impact of silencing TRBP and Dicer on the function of the fully paired and mismatched sequences, we wished to examine Fig. 1. Effect of guide strand 5¢...

Ngày tải lên: 18/02/2014, 06:20

... 3783–3792 ª 2008 The Authors Journal compilation ª 2008 FEBS Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr Robert J. ... whether the move- ment of the P-loop in PhyAsr is due to oxidation of the catalytic cysteine, we oxidized crystals of PhyAsr Table 1. Effect...

Ngày tải lên: 18/02/2014, 18:20

... expression, acetylation of H3 and H4 histones, and the remaining DHSs in the deleted cells were examined to determine the roles of these DHSs on the transcription of Ig-b gene and on the mainten- ance ... of the South- ern hybridization. Position of the probes, the size and position of restriction fragments hybridizable with the probe, and exon ⁄ in...

Ngày tải lên: 19/02/2014, 16:20

... pore [10]. Results about the effect of the luminal (trans) calcium on the activity of the RyR1 are controversial depending on experimental conditions. Inhibition of the channel was described ... Gd 3+ and a Hill coefficient of 4.7 ± 0.8. We have also tested the effect of Gd 3+ on the lumi- nal side of the reconstituted channel. To decrease the possibil...

Ngày tải lên: 19/02/2014, 16:20

... under low ionic strength conditions [8,44]. However, this effect is not seen in the kinetics of the MA/CA hydrolysis. In the high ionic strength of the assays the dimerization is strengthened as ... gene. Many of these mutations are located in the substrate binding site of the PR, and these mutations have considerable impact on PR activity and specificity....

Ngày tải lên: 21/02/2014, 03:20

... 97.4 3228.9 837.7 estimated. In the second approach, on the other hand, as described in the previous section for the case of acquisition from (pseudo-) parallel cor- pora, the candidates of bilingual term ... measured, and their curves against the changes of Ld are shown in Figure 2. Let DP„f denote the set of reference article pairs within the range of dates...

Ngày tải lên: 22/02/2014, 02:20

... neuron degeneration In 2001, Oosthuyse et al. [18] reported that deletion of the hypoxia-response element of the VEGF-A gene promoter and a reduction in VEGF-A expression cause the degeneration of ... tissue fluid and edema in the brain may be dangerous because the volume of the brain tissue is tightly limited by the cranial bone. This limitation is unique among tissu...

Ngày tải lên: 18/02/2014, 11:20

... polarization is further evidence that the functions of Vrp1p in endocytosis, cytokinesis, and growth at elevated temperature, on the one hand, and in cortical actin-patch polarization, on the other hand, ... level of the untagged pro- teins, but we expect that the relative expression level of the tagged proteins is indicative of that of the equival- ent untagge...

Ngày tải lên: 18/02/2014, 16:20