Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

... FEBS Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases Viktoriya S. Sidorenko 1 , Grigory V. Mechetin 1 , Georgy A. Nevinsky 1,2 and ... independently evaluate the effects of ionic strength and Mg 2+ on the activity and specificity of OGG1, we measured the apparent values of k 2 an...

Ngày tải lên: 18/02/2014, 18:20

... Another way of classifying ap- proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same struc- ture as the ... makes them either supervised or unsupervised algorithms. The purpose of this paper is an analysis of the underlying probabilistic models and the types of errors committ...

Ngày tải lên: 20/02/2014, 04:20

... VLCFA of which 18% of the total fatty acids are in the form of 20:1 and 2% in the form of 22:1 [11]. In B. napus seed oil, 62% of the total fatty acids are monounsaturated VLCFA, 10% as 20:1 and ... portion of these enzymes is involved in substrate binding which is consistent with the membrane localization of the substrates of these desaturases [24]. If the r...

Ngày tải lên: 21/02/2014, 15:20

... interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accessions with respect to their metabolic acclimation strategies. ... this function. The flux rate of CO 2 into sucrose synthesis (r CO 2 !Suc ) was caclulated as the difference between the rate of net photosynth...

Ngày tải lên: 14/02/2014, 22:20

... incubated with the reactivase in the presence of ADP and Mg 2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes ... Positions of DD and its subun- its (a D , b D and c D ) and the reactivase (DD-R) and its subunits (a R and b R ) are indicate...

Ngày tải lên: 15/02/2014, 01:20

... [1]. In 1984, the cDNA of human EGFR was isolated and characterized. A high degree of similarity was found between the amino acid sequence of EGFR and that of v-erbB, an oncogene of the avian erythroblastosis ... gefitinib and erlotinib. Here, we review the discovery of EGFR, the EGFR signal transduction pathway and mutations of the EGFR gene in lung cancers...

Ngày tải lên: 16/02/2014, 09:20

... membrane. Together, our findings contribute to the understanding of the role of KCNE4 as a potentially important regulator of KCNQ1 and other K V channels. Results Characterization of KCNE4 antibody A ... KCNE proteins, and the overlapping expression patterns of these subunits [22], there may be multiple and diverse KCNE–KCNQ1 interactions within the same cells or tissu...

Ngày tải lên: 18/02/2014, 17:20

... the ligand-macromolecule reaction occured. A binding isotherm was fitted to the data, expressed in terms of the heat change per mole of ligand against the ligand to macromolecule ratio. From the ... stefin A and stefin B at pH 5 and variant 2 of ste- fin B and the P79S mutant of the variant at both pH 7 and pH 5. Each panel is a plot of heat change on ligand additi...

Ngày tải lên: 19/02/2014, 06:20

... alog (pH ) pK a2 ), Lim1 is the lower limit of the curve, Lim 2 is the middle limit of the curve and Lim 3 is the upper limit of the curve. Stopped-flow kinetic studies of the oxidative half-reaction ... and b subunits) in SDS ⁄ PAGE and migration is identical to that observed for the native enzyme. The migration of both subunits is consistent with the predi...

Ngày tải lên: 19/02/2014, 07:20

... studies. The high selectivity of the a-conotoxins, together with the possibility of obtaining detailed information on their three dimen- sional structures and the relative ease of synthesis, makes them ... native and heterologously expressed nAChRs The examples presented above clearly demonstrate the usefulness of a-conotoxins in the determination of the structur...

Ngày tải lên: 19/02/2014, 12:20