Tài liệu Báo cáo khoa học: Seed-based systematic discovery of specific transcription factor target genes pptx

... Number of genes Number of genes Number of genes Number of genes Fig. 5. Left column: cross-validation of the seed distribution method for six different transcription factors. By means of a jack- knife ... target prediction based on a seed set of known target genes of a specific transcription factor. The target prediction is based on a combination of...

Ngày tải lên: 18/02/2014, 18:20

... regulation of transcriptional activity of nuclear factor kappaB [19], the regulation of NAD(P)H oxidase [20], the shedding of human thyrotropin receptor ectodomain [21], the aggregation of Cu ⁄ ... vivo, there is often a need to use specific inhibitors. Bacitracin is very widely used as an inhibitor of pro- tein disulfide isomerase (PDI) in vivo. However, the specificity of act...

Ngày tải lên: 16/02/2014, 14:20

... consists of 15 genes located on human chromosome 19q13.4. KLK11 ⁄ hippostasin is a member of the kallikrein family and is expressed in various tissues. Two types of KLK11 isoforms, isoform 1 and isoform ... known as a marker protein of Golgi apparatus, showed the localization of isofrom 2– GFP at the Golgi apparatus. Both isoform 1–GFP and A B Fig. 2. Tissue -specific expression...

Ngày tải lên: 19/02/2014, 06:20

... only 20%, despite the presence of ATP- Mg. It is shown below that this is due to loss of the inhibitory effect of ATP-Mg, consequent to the removal of a heat-stable cofactor present in the high- speed ... 2006 FEBS Role of a heat-stable cofactor in the sensitivity of UDP-glucuronidase to metyrapone and other xenobiotics The data obtained with purified microsomes suggested that a co...

Ngày tải lên: 19/02/2014, 07:20

... amplitude of the slow phase (extent of ligation) is independent of the concentration of ATP, approaching % 90% of the total concentration of the ndsDNA substrate in the reaction mixture. Effect of Mg 2+ concentration Mg 2+ is ... member of the same superfamily of nucleotidyl- transferases [34,35]. In the cell, capping of the 5¢-end of mRNA ensures protection from degrada...

Ngày tải lên: 21/02/2014, 00:20

... the processing of apicoplast protein leader sequence. Inhibition of any of these activities could contribute to MA interference in the maturation of the parasite. Polypharmacology of MA The observed ... investigate the extent of possible multi- targeted inhibitory activities of MA, a computational screening was carried out to identify potential binding targets for MA. Here, in s...

Ngày tải lên: 14/02/2014, 14:20

... deacetyla- tion, and transient recruitment of remodellers and transcription factors. A cycle of transcription commences with the recruit- ment of transcription factors and co-factors bound at the promoter, ... recruitment of MOF to recently transcribed regions, especially at the 3¢ ends of genes where H3-K36me3 is enriched [128]. In mammalian cells MOF also exists as part o...

Ngày tải lên: 14/02/2014, 18:20

... types of organisms, including the vast major- ity of eukaryotes and many prokaryotes. It is well established that Sco pro- teins in eukaryotes are involved in the assembly of the Cu A cofactor of mitochondrial ... important factors which relate to the aggregation state of human Sco1 and Sco2 and of their complex with apo-Cox2. It has been proposed that the maturation of Cox2 is...

Ngày tải lên: 14/02/2014, 18:20

... full-length v-KIND; DKIND1, deletion of KIND1; DKIND2, deletion of KIND2; DKIND1 + 2, deletion of both KIND1 and KIND2; DRasN, deletion of RasN; DGEF, deletion of RasGEF; KIND1, KIND1 domain; KIND2, KIND2 ... pull- down assay of combinations of EGFP-fused KIND1 or KIND2 of v-KIND with GST-fused CD2-1-6-1 or CD2-1-6-2 of MAP2. We successfully detected a pull down for the comb...

Ngày tải lên: 14/02/2014, 19:20

... post-transcriptionally by binding to target mRNAs in a sequence -specific manner. A large number of genes appear to be the target of miRNAs, and an essential role for miRNAs in the regulation of ... of the genome and post-translational modification of gene products. The discovery of microRNAs (miRNAs), which regulate gene expression post-transcriptionally, has added to the...

Ngày tải lên: 14/02/2014, 19:20