Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc

... (2008) 2605–2616 ª 2008 The Authors Journal compilation ª 2008 FEBS Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells Mee Hyang Ko 1,2,3 , ... cloned the upstream region of miR9 and studied the expression pattern of endogenous pri-miR9-1 to try to understand miR9 biogenesis during...

Ngày tải lên: 18/02/2014, 17:20

... into COS-7 cells with the Hsp105b expres- sion plasmid, the activation of the hsp70 promoter by Hsp105b was not observed (Fig. 2B). On the other hand, after deletion of the sequence of the hsp70 ... Hsp105b stimulated the phosphorylation of STAT3 at Tyr705 and its translocation to the nucleus. Downregulation of STAT3 expression resulted in reduction of the...

Ngày tải lên: 18/02/2014, 06:20

... E22K, E22Q and D23N), and the availability of a rapid and simple expression and purification protocol will facilitate large-scale investigations of the molecular determinants of aggregation and fibrillation. ... significantly increases the cost of the purification, and the require- ment for HPLC increases the length and complexity of the purification procedu...

Ngày tải lên: 18/02/2014, 13:20

... independently of the presence of AREs (Fig. 6B, lanes 2 and 5). It is possible that in the region located before the AREs, consisting of 343 bp of the 3¢ UTR of the IL-1b transcript, there is a putative ... lower levels of IL-1b mRNA are due to shortening of the IL-1b transcript half-life, and are not related to the presence of AU-rich elements in the 3¢ UT...

Ngày tải lên: 18/02/2014, 14:20

... conserved His and the hydroxyl of the active-site Ser increases the nucleophilicity of the Ser oxygen, facilitating attack of the hydroxy group on the silicon atom of the substrate. This reaction ... bonds, the concentration of the released product p-aminophenol was determined at a wavelength of 300 nm, which is in the trailing edge of the main absorption...

Ngày tải lên: 18/02/2014, 16:20

... analysis, and the determination of released ammonia. The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step. The product ... increased during this incubation time. The peak at 268 nm was assigned to 2,5-pyridinedicarboxylic acid based on the wavelength [10]. Purification and propert...

Ngày tải lên: 19/02/2014, 16:20

... tasks, and up- loads them to the MTurk Web-site. Workers can then browse the tasks and, if they find them profitable and/ or interesting enough, work on them. When the tasks are completed, the employer ... the ones corresponding to the questions (84 documents). The source of Wik- iXML is the English part of the Wikipedia XML Corpus by Denoyer and Gallinari (2006). In...

Ngày tải lên: 20/02/2014, 09:20

... the set of case elements that modify the i-th verb in T rs i (T ) : the set of particles in the set of case el- ements that modify the i-th verb in T ns i (T ) : the set of nouns in the set of ... )) the co-occurrence prob- ability of the particle set and the verb, and we call P(n i,j (T )|r i,j (T ), v i (T )) the co-occurrence probability of the cas...

Ngày tải lên: 20/02/2014, 12:20

... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurrence of a transition between 35° and 55 °C. PHI ... the first hypothesis and therefore a direct involvement of PHI in electron transfer. This conclusion is consistent with the very low degree of identity between the...

Ngày tải lên: 21/02/2014, 00:20

... between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b and cENaC, leading to channel ubiquitination, internalization and degradation ... donor (Fig. 8A), the inhibitory effects of heparin and DPG (Fig. 8B), the fact that purified CK2 can phosphorylate bS631 and cT599 (Fig. 7A), the presenc...

Ngày tải lên: 21/02/2014, 03:20