Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

... 112–116. Study of the interactions of the Anp32a LRR module C. de Chiara et al. 2560 FEBS Journal 275 (2008) 2548–2560 ª 2008 The Authors Journal compilation ª 2008 FEBS Structural bases for recognition of ... understanding of how interactions could take place in different regions of the molecule. As part of a long-term function-oriented structural effort aimed at unders...

Ngày tải lên: 18/02/2014, 17:20

... crystallography. Biochemistry 40, 2754–2765. 27 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem ... respectively, of that of HPRT. This low activity could be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In P...

Ngày tải lên: 15/02/2014, 01:20

... an accurate measure of the k off parameter in CaM-bound eNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational change rates versus rates of FMNH 2 formation in ... rate of FMNH 2 for- mation appears to be relatively fast and not rate limit- ing, and instead a specific conformational step (k off , dissociation of the reduced FMN subdomain) i...

Ngày tải lên: 18/02/2014, 11:20

... Enterobacter cloacae. For reference, in Table 1, we summarize the flavoproteins for which a specific H-tunnelling study has been performed. Gener- ally, the KIEs of H transfer in flavoproteins are fairly modest ... following the formation of this CT absorbance at, for example, 555 nm while perform- ing a concentration dependence [35,39]: k obs ¼ k off þ k on ½NADðPÞHð6Þ Similarly, the...

Ngày tải lên: 18/02/2014, 11:20

... potentials of the fla- vin in FNRs are close to each other, therefore these proteins stabilize only 10–20% of the maximal amount of semiquinone [63,64]. An E ox ⁄ hq value of )325 mV has been reported for ... Bradley LH & Swenson RP (1999) Role of glutamate- 59 hydrogen bonded to N(3)H of the flavin mono- nucleotide cofactor in the modulation of the redox potentials of t...

Ngày tải lên: 18/02/2014, 11:20

... (1970) Cleavage of structural proteins dur- ing the assembly of the head bacteriophage T4. Nature 227, 680–685. 23 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram ... rmsd of 1.29 A ˚ for 212 Ca-atoms. The binding of UMP in E. coli creates a tightly closed conformation with a2 (Fig. 4A). In UpUMPK, this a-helix has a more open conformation i...

Ngày tải lên: 18/02/2014, 16:20

... positions and the conformations of the three loops of EcoMBP are different from those of TvuCMBP. Trp62, located at region N-I, is found at the center of the curved form of maltotetraose (Fig. ... architecture of the C-domain of Tli TMBP may be more s uitable for the specific b inding to small oligosaccharides lik e trehalose. Comparison of the N-domain parts of the sugar-b...

Ngày tải lên: 19/02/2014, 00:20

... ionic strength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD) seems to favor formation of dodecahedra over isolated pentamers. Previous structural analysis of the ... the 60 copies of the full-length protein. The formation of small subviral particles by truncated viral capsid proteins is not limited to adenovirus. For exam- ple, thi...

Ngày tải lên: 19/02/2014, 06:20

... alteration of this ratio for a mutant was considered as caused either by an alteration of the K m value for one of the two substrates or by a variation of the k cat value for one of the two activities. ... [11]. Mutation of two residues involved in the binding of adenine (F127E and M436W) had no effect on the functional characteristics of the enzyme, except for an incr...

Ngày tải lên: 15/02/2014, 01:20

... previously demonstrated the application of this method to the determination of the glycan structure of a form of AFP [10]. However, identification of the details of a glycan structural change on a glycoprotein ... microarray system is the best tool for a ‘cell profiler’, and it is expected to be applicable for selection of cancer-specific lectins and for quality control of...

Ngày tải lên: 16/02/2014, 08:20