... Enterobacter cloacae. For reference, inTable 1, we summarize the flavoproteins for which aspecific H-tunnelling study has been performed. Gener-ally, the KIEs of H transfer in flavoproteins are fairlymodest ... following the formation of thisCT absorbance at, for example, 555 nm while perform-ing a concentration dependence [35,39]:kobs¼ koffþ kon½NADðPÞHð6ÞSimilarly, the rate of hydride transfer ... of the 1° KIE of an OYE mayrequire $100 mg of isotopically pure substrate. For reference, we briefly describe our preferred methods of synthesis for large-scale ($1 g) preparations of allthree...