Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx

... from Caveolin-1 cDNA are GCAAGTGTACGACGCGCAC and AACCAGAAGGGACACACA, respectively. As negative controls (scrambled shRNAs), shRNAcontrol 1 (TAGCGACTAAACACATCAA), shRNAcontrol 2 (TATA GCGACTAAACACATCAA) and ... rafts are microdomains of the plasma membrane containing a high proportion of sphingo- lipids and cholesterol, assembled in the Golgi apparatus and subsequently deli...

Ngày tải lên: 19/02/2014, 00:20

... (i.e. the b-hairpin loop and part of the adjoining b-sheet) and residues in the loop connecting b-strand and a- helix and contiguous residues on the a- helix and the last part of b-strand 3Õ [35]. ... membrane. The way this contact takes place and the molecular features of the protein involved are yet to be deciphered. The lack of information about...

Ngày tải lên: 20/02/2014, 11:20

... 2815 (5¢-CCGAGCTCGAGACGTGAAGCGACAATCTCGC AATCT-3¢) containing an XhoI (Promega) site upstream of the start codon and an antisense primer (5¢-CAGCCAA GCTTCTCACTCTTTGATGAAATGCATCT-3¢) con- taining a HindIII ... Takashi Isobe 1 , Takeshi Arakawa 2 , Yasunobu Matsumoto 3 and Naotoshi Tsuji 1 1 Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricult...

Ngày tải lên: 20/02/2014, 11:20

... considered as follows. The role and importance of the aspartate in the catalytic triad is not fully understood because several serine proteases do not have an aspartate as the catalytic apparatus. However, ... dependence; the acidic rim is at pK a ¼ 6.5 and the Fig. 1. Stick models of the reactive site in bovine trypsin and API. The catalytic triad residue...

Ngày tải lên: 21/02/2014, 03:20

... beta- and gamma-catenins in their cytoplamic tails. As with tight junctions, adherens junctions are linked to the actin cytoskeleton via the binding of beta- and gamma-cate- nin to alpha-catenin ... in ammatory responses [85]. In addition, the slight edema of astro- cytes helps maintain the water balance in the brain, but pathologic cytotoxic edema is a main cause...

Ngày tải lên: 18/02/2014, 11:20

... qPCR. Transcript abundance values for AaEcRA, AaEcRB, AaUSP -A, AaUSP-B, AaE7 5A (A) , AaHR3, AaHR4, AaE78, AaHR39, AaHR78 (B) and AabFTZ-F 1A, AabFTZ-F1B, AaHNF- 4A, AaHNF-4B, AaHNF-4C (C) are presented ... to increase again at peak vitellogenesis (18 + 24 h PBM). These transcripts (AaUSP -A, AabFTZ-F 1A , AabFTZ-F1B, AaHR78, AaHNF- 4A, AaHNF-4B, AaHNF-4C, AaSvp and AaERR) were also...

Ngày tải lên: 18/02/2014, 13:20

... the identifiable states of the folding pathway and relate to the amount of solvent-exposed surface area in each of these states (see Materials and methods). This can be done for all five states in the ... study, it was ascertained that the first reaction phase was fast and concentration dependent, showing that the intermediate was readily populated and dimeric. The...

Ngày tải lên: 18/02/2014, 13:20

... cellular ceramide are regulated by the de novo pathway and the recycling pathway. The former relates to the synthesis of ceramide through the conden- sation of palmitate and serine in a series of ... mutations. Acknowledgements This research was supported in part by the Intramural Research Program of the National Institute on Aging, National Institutes of Hea...

Ngày tải lên: 18/02/2014, 14:20

... organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain Thirumaran Thanabalu 1,2 , Rajamuthiah Rajmohan 2 , Lei Meng 2 , Gang Ren 4,5 , Parimala R. Vajjhala 4 and ... and immunoblotted with an anti-actin mAb (left). Equivalent amounts of purified yeast actin were used in each binding assay and an amount representing 10% of t...

Ngày tải lên: 18/02/2014, 16:20

... circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderw- altozyma and Zygotorulaspora. FEMS ... due to amino acid biosynthesis. Much of the generation of NADH during amino acid biosyn- thesis takes place in the mitochondria. Because of the block i...

Ngày tải lên: 19/02/2014, 02:20