Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside

Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... report the characterization of the pyrimidine-speciﬁc ribonucleoside hydrolase from the hyperthermophilic archaeon Sulfolobus solfataricus (SsCU-NH). The gene SSO0505 encoding SsCU-NH was cloned and expressed ... from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling Marina Porcelli 1,2 , Luigi Concilio...

Ngày tải lên : 18/02/2014, 17:20

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Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

... FEBS reduced (residues 1 2–1 6) from the starting structure (residues 1 1–1 9). At the end of the simulation, the CT-extension was isolated by deleting the mature domain and the extension was allowed ... latex enzymes Erv-A, -B and -C from the same plant, and papain from papaya latex and two other serine (tryp- sin and chymotrypsin) and one aspartic (pe...

Ngày tải lên : 14/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... 1657 Discussion The present study revealed the structural and func- tional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e. the MAP2-binding core module in v-KIND and the v-KIND-binding ... not KIND1, and was not observed for the KINDs from other KIND-containing proteins. The binding core modules critical for the v-KIND–MAP2 interaction w...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... 30821065 and 30870559 from the China National Nature Sciences Foundation, 2006CB910703 from the Ministry of Sciences and Technology of China, and 07dz05907 from the Committee of Sciences and Technology ... adipocytes, the translo- cation of Bax from the cytoplasm into the mitochon- dria, cytochrome c release and cleavage of caspase-3 were all observed (Fig. 4B–D)...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... lysates were then immunoblotted for GFP to reveal Bid–GFP and tBid–GFP. (E) HEK-293T cells were transfect- ed with tBid–GFP and Myc–ubiquitin in the presence or absence of FLAG–Itch and treated ... rLuc–Itch and various amounts of either Bid–GFP of tBid–GFP. The graph is a representative example of the saturation studies performed to provide evidence for a speciﬁc interaction...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... b¢, and a¢, a linker region x between the b¢ and a¢ domains, and a C-terminal acidic extension. The a and a¢ domains contain the CGHC active site motif, and are sufficient alone to perform thiol–disulfide ... mechanism of action, the reaction conditions of the assay were var- ied. The removal of EDTA from the reaction signifi- cantly reduced the noncatalyzed rate (Fig...

Ngày tải lên : 16/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Myristoylation of the dual-speciﬁcity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

... 246 3–2 473 ª 2010 The Authors Journal compilation ª 2010 FEBS from whole cell lysates and analyzed by nanoﬂow LC ⁄ ESI-MS ⁄ MS. The tandem mass spectrum of the N-terminal tryptic peptide GNGMNK from ... dimethylsulfoxide as the control. Cells detached from the culture dish were collected, stained with Trypan Blue and counted. The results are the mean of three experi...

Ngày tải lên : 16/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... concentrations, the ratio between the bands at 1618 and 1638 cm )1 is plotted in Fig. 5B. The b-sheet contribution was most signiﬁcant when the SDS concentration was 1–6 mm, reaching a maximum at 2–4 mm, ... C-peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C-peptide in the presence of insulin (C) and of 100 l M C-p...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... into mutants IV and VI had little effect relative to the wild-type protein (Fig. 3A–C, insets). Figure 3D–F shows the equilibrium binding analysis of the protein– membrane interactions, and the K D values ... inside out, exposing the hydrophobic x-loop to solvent and burying the majority of the Gla residues. Of the seven Ca 2+ , the majority are buried and are inte...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through speciﬁc protein interactions in the methionine synthase–methionine synthase reductase complex docx

... inset). The absorption spectrum of the puriﬁed enzyme was typical of the hydroxycobalamin form of the enzyme (Fig. 2). The recovery of the activity was  10%, and the enzyme was puriﬁed 3669-fold. The ... present in the assay may be reducing FMN and ⁄ or the cobalamin. The source of the reducing agent is unknown, but it may originate from dithionite on the surf...

Ngày tải lên : 18/02/2014, 08:20

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