Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt

... used to establish that KCNE4 directly associates with KCNQ1, and can co-associate together with KCNE1 in the same KCNQ1 complex to form a ‘triple subunit’ complex (KCNE1–KCNQ1 KCNE4) . We also used ... interaction of KCNE4 with KCNQ1. This hypothesis was tested by examining whether KCNE4 forms protein complexes with KCNQ1. KCNE4 and KCNQ1 were transiently co-expresse...

Ngày tải lên: 18/02/2014, 17:20

... to the type I and type II receptors on the cell surface. These receptors are serine ⁄ threonine kinase receptors with a single transmembrane domain. Bind- ing of the ligand to the receptor complex ... complex induces the type II receptor to phosphorylate the type I receptor, which then leads to activation of the type I receptor. The signal is passed on to the substrate...

Ngày tải lên: 19/02/2014, 00:20

... of the long desk is a chair. Another chair is to the left of the long desk. The chair in front of the desk is near the short desk." OTHER APPROACHES AND CLOSING REMARKS Nearly all the ... Intuitively, the closer the "stool" is to the region of space defined by the projection of the desk's dimensions into this space, the more the spatial a...

Ngày tải lên: 20/02/2014, 21:20

... help the users to discern essential information with unparalleled clarity. The “Technologies behind the system” chapter describes the implementation and the integration approach of the core ... managing dynamic content- driven websites with a linguistic platform. The platform enriches the content of these websites with revealing details and reduces the manual wor...

Ngày tải lên: 22/02/2014, 03:20

... thetase to esterify the 13cROL generated in the reac- tion. Therefore, 13cROL is accumulated in the absence of LRAT in the reaction (Fig. S3B). Under the in vitro assay conditions of the ... washes with NaCl ⁄ Tris with Tween 20, the membrane was then incubated for 1.5 h with a 1 : 25 000 dilution of anti-mouse IgG conjugated with Dy- Light 549 and anti-rabbit IgG conjug...

Ngày tải lên: 14/02/2014, 14:20

... mitochondria during apoptosis. They induce the permeabilization of the mitochondrial membrane with the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic protein Bax ... mitochondria and then translocate to the nucleus where they induce DNA fragmentation in a caspase-independent manner. Whether apoptosis is induced or not depends on the...

Ngày tải lên: 14/02/2014, 22:20

... enthalpy is the sum of two terms: the reaction enthalpy, independent of the buffer used in the experiment, and another term representing the contribution of the proton ionization of the buffer, which ... HCl, 1 mM MgCl 2 , 100 mM NaCl. The upper panels show the raw data for the heat effect during the titrations; the lower panels are the binding isotherms. Table 2. Th...

Ngày tải lên: 16/02/2014, 14:20

... another factor or other signals are required to fully derepress Meis2d. The Meis2e splice variant retains the Hth domain, but lacks both the HD and the AD. It could therefore interact with Pbx, but ... lines. It may therefore represent a significant proportion of the functional Meis3 protein. However, further work will be required to determine the relative levels of the protein...

Ngày tải lên: 16/02/2014, 15:20

... We prepared the 46-nucleotide DNAzyme with DNA-cleaving activity (PL DNAzyme), and then grafted the deoxyribonucleotide residues from an 8–17 variant DNAzyme into the region of stem–loop I and the catalytic core ... ssDNA [21]. The 5¢-arm of the enzyme binds the cleavable sequence via Watson–Crick base pairs and the 3¢-arm through formation of a DNA triplex. The catalytic...

Ngày tải lên: 16/02/2014, 15:20

... agreement with the results of the NMR. The addition of SDS to the C-peptide sample leads to formation of larger objects, even at an SDS concentra- tion of only 500 lm, which is well below the CMC. The ... about the calcu- lated population distributions cannot be made. The DLS experiments, however, do confirm the formation of C-peptide oligomers in the presence of SDS. The...

Ngày tải lên: 18/02/2014, 04:20