... directly. Nevertheless, this assaywas near the limit of detection for this interaction, assuccessive dilution of the probe soon led to loss of sig-nal. The low yields of the AP probe and of nucleolin,together ... shown).Further perturbation experiments were carried outusing the protein lactoferrin. Lactoferrin, an iron-binding protein of the transferrin family, is presentin external secretions and the secondary ... incubated with varying concentrations of condi-tioned media containing FN3d–AP. The data revealedsignificant binding of PTPr to nucleolin above that of the BSA control (Fig. 3), demonstrating that PTPrcan...