Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... MINIREVIEW Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS Namrata D. Udeshi1, Jeffrey Shabanowitz1, Donald F. Hunt1,2 and Kristie ... radical anion abstracts a proton and gen-erates a radical site that triggers dissociation to pro-duce a complementary pair of fragment ions of type c¢ and z¢Æ. Subtraction of the m ⁄ z values ... mixtures on a chromatographic timescale (500 ms ⁄ protein).AbbreviationsCAD, collision-activated dissociation; ECD, electron-capture dissociation; ESI, electrospray ionization; ETD, electron-transfer...
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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... 5¢-GCGAATTCGTTGAAAACTTTTAATTATCAGGAGAAAAC-3¢,5¢-CGAGATCTAGCATGTCAGACGTGGAGTCTGGA-3¢ and 5¢-GCGAATTCGCAACCAAAGACAACCTGGTTTTAATGTTTTGA-3¢, and 5¢-CGAGATCTGAAATGAACGAGCTGCGTATGCCGAA-3¢ and 5¢-GCG AATTCAAC ACAAGAG TTGT TTTATAT TGAACCCA-3¢, respectively. ... sequences of olvas, nanos and tudor weremodified by PCR with BglII and EcoRI, using primers5¢-GGAGATCTAAAATGGACGACTGGGAGGAAGA-3¢ and 5¢-GCGAATTCGTTGAAAACTTTTAATTATCAGGAGAAAAC-3¢,5¢-CGAGATCTAGCATGTCAGACGTGGAGTCTGGA-3¢ ... described above was employed as a template for PCR, using primers 5¢-GCGCTAGCTAATACGACTCACT ATAG GGA GATC TAAA ATGG AC GACTGGGAGGAAGA-3¢ and 5¢-GCGAATTCGTTGAAAACTTTTAATTATCAGGAGAAAAC-3¢. This...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... cleavage of mutated htt and the release of an N-terminal fragment bearing themutation that is capable of nuclear localization [6,7].HttEx1-polyQ N-terminal fragments with repeats of fewer than ... Anatomy and Neurobiology, Dalhousie University, Halifax, CanadaNeuronal selective loss and formation of intraneuron-al protein aggregates are characteristics of Hunting-ton’s disease (HD), ... treatmentwith an antioxidantWance J. J. Firdaus1, Andreas Wyttenbach2, Chantal Diaz-Latoud1, R. W. Currie1,3 and Andre´-Patrick Arrigo11 Laboratoire Stress Oxydant, Chaperons et Apoptose,...
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Tài liệu Báo cáo khoa học: "ANALYSIS OF OONOUNCTIONS IN PAKSER" potx
... della Babblica Istruzione of Italy, MPI 40% Intelligenza Artificiale. the conjunctions are handled by m~ans of a para- syntactic mechanis~ that enables the parser to analyze the second conjunct ... interpretation of sentences containing conjunctions in great detail. There- fore, it is reasonable to assume that in many of these systems the conjunctions are handled only by means of specific ... constitutes the syn- tactic component of the FIDO system (a Flexible Interface for Database Operations), an interface allowing an end-user to access a relational data- base in natural language...
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Tài liệu Báo cáo khoa học: Mechanisms of obesity and related pathologies: Androgen deficiency and endothelial dysfunction may be the link between obesity and erectile dysfunction pptx
... its pharmacogenetics on physical performance and metabolism. Asian J Androl 10, 364–372.112 Yassin AA, Saad F & Traish A (2006) Testosteroneundecanoate restores erectile function in a subset ... T, Berman N,Hull L & Swerdloff RS (2004) Long-term testosteronegel (AndroGel) treatment maintains beneficial effects on sexual function and mood, lean and fat mass, and bone mineral density ... regarding thispathophysiological state.Central (abdominal) obesity, as assessed by severalcharacteristics including BMI, WC and WHR, is con-sidered as a hallmark of the MetS and is associatedwith...
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Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
... siRNA at a final concentration of 10 nM. Fluorescence was measured 24 h aftertransfection. The mean and standard deviation are shown for each condition. Asterisks indicate that the two-tailed ... vitroexperiments have shown that TRBP, Dicer and Ago2 alone are capable of forming an active mini-mal RLC [13].Being double-stranded, either strand of the siRNAcan be used as the guide strand of an active ... Zerivitz K & Akusjarvi G (1995)Small-ScalePreparation of Nuclear Extracts from Mammalian Cells.Academic Press, London.Supporting informationThe following supplementary material is available:Fig....
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Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf
... INSERM,Paris.13 Tadokoro T, Matsushita K, Abe Y, Rohman MS, Koga Y, Takano K & Kanaya S (2008) Remarkablestabilization of a psychrotrophic RNase HI by combi-nation of thermostabilizing mutations ... reaction. Biotechniques 8, 528–535.34 Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E,Nakamura H, Katayanagi K, Morikawa K & IkeharaM (1991) Stabilization of Escherichia coli ribonucleaseH ... The Authors Journal compilation ª 2008 FEBS 613Destabilization of psychrotrophic RNase HI in a localizedfashion as revealed by mutational and X-raycrystallographic analysesMuhammad S. Rohman1,...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... expression and regulation of PrPCin consideration of the di-, mono- and nonglycosylated protein bands.For distinct discrimination among various species,such as mouse, sheep, cattle and humans, ... of glycoform ratios are detectable. The findings areimportant for studies of PrPCfunction, regulation and expression, as full-length and truncated isoforms of di-,mono- and nonglycosylated proteins are ... PrPsignals for the di- (d), mono- (j) and non-glycosylated (m) isoform were defined as100% and the contribution of each bandwas calculated as percentage. Linearity inthe range of 4–10 lL of brain...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... demonstrating that the conformationalchanges occurring upon ligand binding are accompanied by significant heat capacity changes.Determination of the heat capacity changes for the K22V,R120K and ... data obtained at this temperature was not includedin the analysis. The raw data were integrated and normal-ized for molar concentration. The dissociation constants, Kdvalues, enthalpies of ... to V): 5¢-GATATGCAGGTCAACAGGAACCGCGCCAATGGCGCAACC-3¢.The template used for amplification was the pKK233-2plasmid containing Enterobacter cloacae MurA wild-type(for generation of the R120K...
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Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
... temperature stability of the protein.Results Analysis of the amino acid sequenceMetabolic pathway reconstruction based on genomedata suggested the presence of a nonphosphorylatedvariant of the ... unable toreach saturation of the enzyme. A broad range of aldose sugars was tested as poten-tial substrates for GdhA. The enzyme was significantlyactive only with d-galactose, reaching 74% of ... the ratio of enzymaticactivity with NAD+ and NADP+as cosubstrates,which was 1 : 20 in both cases, as well as the ratio of d-glucose ⁄ d-galactose oxidation rates (Table 2).Also, upon native...
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