Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt

... MINIREVIEW Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS Namrata D. Udeshi 1 , Jeffrey Shabanowitz 1 , Donald F. Hunt 1,2 and Kristie ... radical anion abstracts a proton and gen- erates a radical site that triggers dissociation to pro- duce a complementary pair of fragment ions of type c¢ and...

Ngày tải lên: 18/02/2014, 16:20

... 5¢-GCGAATTCGTTGAAAACTTTTAATTATCA GGAGAAAAC-3¢,5¢-CGAGATCTAGCATGTCAGACG TGGAGTCTGGA-3¢ and 5¢-GCGAATTCGCAACCAAA GACAACCTGGTTTTAATGTTTTGA-3¢, and 5¢-CGAG ATCTGAAATGAACGAGCTGCGTATGCCGAA-3¢ and 5¢-GCG AATTCAAC ACAAGAG TTGT TTTATAT TGAA CCCA-3¢, respectively. ... sequences of olvas, nanos and tudor were modified by PCR with BglII and EcoRI, using primers 5¢-GGAGATCTAAAATGGACGACTGGGAGG...

Ngày tải lên: 18/02/2014, 16:20

... cleavage of mutated htt and the release of an N-terminal fragment bearing the mutation that is capable of nuclear localization [6,7]. HttEx1-polyQ N-terminal fragments with repeats of fewer than ... Anatomy and Neurobiology, Dalhousie University, Halifax, Canada Neuronal selective loss and formation of intraneuron- al protein aggregates are characteristics of Hunting- ton’s...

Ngày tải lên: 19/02/2014, 06:20

... della Babblica Istruzione of Italy, MPI 40% Intelligenza Artificiale. the conjunctions are handled by m~ans of a para- syntactic mechanis~ that enables the parser to analyze the second conjunct ... interpretation of sentences containing conjunctions in great detail. There- fore, it is reasonable to assume that in many of these systems the conjunctions are handled only by...

Ngày tải lên: 21/02/2014, 20:20

... its pharmacogenetics on physical performance and metabolism. Asian J Androl 10, 364–372. 112 Yassin AA, Saad F & Traish A (2006) Testosterone undecanoate restores erectile function in a subset ... T, Berman N, Hull L & Swerdloff RS (2004) Long-term testosterone gel (AndroGel) treatment maintains beneficial effects on sexual function and mood, lean and fat mass, and bone...

Ngày tải lên: 18/02/2014, 06:20

... siRNA at a final concentration of 10 n M. Fluorescence was measured 24 h after transfection. The mean and standard deviation are shown for each condition. Asterisks indicate that the two-tailed ... vitro experiments have shown that TRBP, Dicer and Ago2 alone are capable of forming an active mini- mal RLC [13]. Being double-stranded, either strand of the siRNA can be used as the gui...

Ngày tải lên: 18/02/2014, 06:20

... INSERM, Paris. 13 Tadokoro T, Matsushita K, Abe Y, Rohman MS, Koga Y, Takano K & Kanaya S (2008) Remarkable stabilization of a psychrotrophic RNase HI by combi- nation of thermostabilizing mutations ... reaction. Biotechniques 8, 528–535. 34 Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E, Nakamura H, Katayanagi K, Morikawa K & Ikehara M (1991) Stabilization of Escheric...

Ngày tải lên: 18/02/2014, 13:20

... expression and regulation of PrP C in consideration of the di-, mono- and nonglycosylated protein bands. For distinct discrimination among various species, such as mouse, sheep, cattle and humans, ... of glycoform ratios are detectable. The findings are important for studies of PrP C function, regulation and expression, as full-length and truncated isoforms of di-, mono- a...

Ngày tải lên: 19/02/2014, 02:20

... demonstrating that the conformational changes occurring upon ligand binding are accompanied by significant heat capacity changes. Determination of the heat capacity changes for the K22V, R120K and ... data obtained at this temperature was not included in the analysis. The raw data were integrated and normal- ized for molar concentration. The dissociation constants, K d values, entha...

Ngày tải lên: 19/02/2014, 13:20

... temperature stability of the protein. Results Analysis of the amino acid sequence Metabolic pathway reconstruction based on genome data suggested the presence of a nonphosphorylated variant of the ... unable to reach saturation of the enzyme. A broad range of aldose sugars was tested as poten- tial substrates for GdhA. The enzyme was significantly active only with d-galacto...

Ngày tải lên: 19/02/2014, 16:20