... increased affinity for some lig-ands, such as warfarin [5,18–23].Fatty acids are effective in allosterically regulatingligand binding to Sudlow’s site I and to the heme cleft.Myristate regulates ... Mn(III)hemeaffinity to HSA, whereas warfarin and FA7 ligandswere reported to behave in the opposite way withrespect to ferric heme binding to HSA [19,21,31].Additionally, the affinity of Mn(III)heme for HSA and the ... different myristate bindingsite allosterically coupled to FA1, and FAS are n secondary myristate binding sites, with different affinities, allosterically uncoupled to FA1.P ¼ protein, HSA; Myr...