Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... 2007 The Authors Journal compilation ª 2007 FEBS 4121 Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin- binding ... a novel G -actin- binding domain, which we call a verprolin homology 2 C-terminal (VH2-C) domain; and a second submod- ule comprising th...

Ngày tải lên: 18/02/2014, 16:20

... the artifact may be caused by using a plant translation system, in which mammalian mRNA was inappropriately translated. The transient expression assay indicated that M33S and isoform 1 proteins were ... 5¢-TCATGCTGGTTGAGACTG GA-3¢ and 5¢-CCAGGTGTGGAGCAAGGCAG-3¢) and a 938 bp fragment of promoter 3 (primers, 5¢-CTCACAG CAGCCAGTAAGTG-3¢ and 5¢-ACTCACAGGCTCTGG GGCTG-3¢) were...

Ngày tải lên: 19/02/2014, 06:20

... [53]. Role of the TXT motif and water in activity Examination of the crystal structures of the insect AFPs also revealed the presence of an array of water molecules Fig. 4. Comparison of the insect ... that TmAFP has o ne and a h alf extra coils along the T XT face (Fig. 8A) . This gives TmAFP one and a half additional TXT motifs along the ice-binding fac...

Ngày tải lên: 19/02/2014, 16:20

... com- posed of an extracellular ligand-binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. The binding of a ligand to the extracel- lular domain of the EGFR induces ... dimeriza- tion, activation of the intracellular kinase domain and autophosphorylation of tyrosine residues within the cytoplasmic domain of the receptor....

Ngày tải lên: 16/02/2014, 09:20

... control. The target mRNA was normalized against actin in the same sample. The PCR primers were as follows: actin forward, 5¢-GA AATCGTGCGTGACATCAAAG-3¢; actin reverse, 5¢-TG TAGTTTCATGGA TGCCACAG-3¢; ... proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway Yuanyuan Xiao, Taichang Yuan, Wenqi Yao and Kan Liao State Key Labora...

Ngày tải lên: 16/02/2014, 09:20

... (GRASP55). Further analysis revealed that GRASP55 associated with the cytoplasmic domain of both proteases and that the LLY 573 motif in the MT1-MMP intracellular domain was crucial for the interaction ... for furin-mediated activation of transmem- brane substrates. The activation of ADAM17 by furin [68] and our observation of an interaction between GRASP55 and...

Ngày tải lên: 18/02/2014, 04:20

... N-terminal hexahistidine tag was obtained by PCR using the pET2 1a ⁄ PNT- H6 plasmid [30] as the template. The forward primer (5¢-TACCGTTAACATCGATATGCATCATCATC ATCATCATGC-3¢) was designed to insert ... Zhang X, Buccellato M, Darbon H, Canard B, Finet S & Longhi S (2005) The intrinsically disordered C-terminal domain of the measles virus nucleoprotein interacts with the...

Ngày tải lên: 18/02/2014, 04:20

... beta- and gamma-catenins in their cytoplamic tails. As with tight junctions, adherens junctions are linked to the actin cytoskeleton via the binding of beta- and gamma-cate- nin to alpha-catenin ... vas- culature, because they regulate the formation and main- tenance of BBB, modulate neurovascular coupling and maintain several parts of brain homeostasis. In this mini...

Ngày tải lên: 18/02/2014, 11:20

... of Fundamental Research in Health Science’ of the Organi- zation for Pharmaceutical Safety and Research. In May 2009, the FDA in the USA approved bevacizumab for the treatment of patients with relapsed ... astrocytes, and the tight junctions of vascular endothelial cells (ECs) in the BBB are well organized with claudins and ZO- proteins through a decrease...

Ngày tải lên: 18/02/2014, 11:20

... Rosell A, Alvarez-Sabin J, Arenillas JF, Rovira A, Del- gado P, Fernandez-Cadenas I, Penalba A, Molina CA & Montaner J (2005) A matrix metalloproteinase pro- tein array reveals a strong relation ... The transla- tional attractiveness of this approach lies in the fact that minocycline can be easily used in humans. Taken together, the accumulating experimental and clini...

Ngày tải lên: 18/02/2014, 11:20