Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... prothrombinalready obtains over 70% of its potential binding and protein C mutants II and III each have approximately50%, whereas wild-type protein C has obtained a mere19% of its potential binding. ... membrane-binding domain [9,10]. ThisCa2+binding requires the presence of Gla residues.The Gla domains within the protein C family comprise44 amino acids and contain between nine and 11 Glaresidues, ... and approach that of plasma-derivedhuman prothrombin (1.8 mm), comprising an efﬁcientmembrane-binding Gla protein. Fig. 3. Protein membrane interaction of wild-type and variant mouse protein...

Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

... role in general insulin mRNA stability, as PTB has recently been implicated in insulin mRNA stabilization in normoxic conditions [62]. Interestingly, we observed that theinsulin mRNA PTB binding ... tract binding (PTB) RNAbinding proteins. Analysis of the CSD/PTB binding sitesrevealed a potential role in VEGF mRNA stability, in bothnoninduced and induced conditions, demonstrating a gen-eral ... proteinshave been shown to interact with a number of partnerproteins in solution [22,23].Involvement of the VEGF 3¢-UTR in binding CSD and PTB proteinsAs sequences in the 3¢-UTR are also involved...

Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... conditions of ATP binding and hydrolysis.Here, we summarize the properties of the three protein classes, with the main focus being on RNAchaperones and annealer proteins.Speciﬁcally binding proteinsA ... startingpoints for invasion. The concerted process of opening of the initial double strand and the annealing of thenew duplex ﬁnally results in either the replacement of the original strand ... given insights into the mechanism(s) of DEAD-box helicases. The binding sites for double-stranded RNA and ATP overlap, resulting in coupledbinding of both molecules. Simultaneous bindingforces...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... protein kinase; MAPKK, mitogen-activated protein kinase kinase; MBP, myelin basic protein; OXI1, oxidativesignal-inducible 1; PDK1, 3-phosphoinositide-dependent kinase 1; PTI1, Pto-interacting ... mitogen-activated protein kinases (MAP-Ks), MPK3 and MPK6, in response to oxidative stress. In addition, theserine ⁄ threonine protein kinase Pto-interacting 1-2 (PTI1-2) has beenpositioned ... then examined how both proteinsinteract with MPK3 and MPK6 proteins.ResultsAGC kinases interact with PTI1 kinases in vitroTo isolate other components of the OXI1 (AGC2-1)signalling pathway,...

Tài liệu Báo cáo khoa học: The miRNA-192 ⁄194 cluster regulates the Period gene family and the circadian clock doc

... the control of the circadianclock. The phosphorylation and degradation of Perproteins have been suggested to control timing of themammalian clock [8]. Moreover, BMAL1 and Cryproteins are subject ... transcription,resulting in reduced CLOCK ⁄ BMAL1 levels and decreased CCG expression [7]. This mode of repressionleads to the cycling of BMAL1 mRNA levels in ananti-phase fashion to that of the CCGs. ... sites in the3¢UTRs of their target genes, they can induce transla-tional inhibition or mRNA decay. miRNAs have beenshown to be involved in many cellular processes,including the control of the...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: intersubunit interactions during virus assembly doc

... of the precise residues and interac-tions involved in the NTD–CTD interface [46]. Most of these involve side chains from helix 8 of the CTDwhich pack against the N-terminal end of helix 4 of the ... capsid protein (CA) of HIV-1 is composed of two domains, the N-ter-minal domain (NTD) and the C-terminal domain (CTD). During theassembly of the immature HIV-1 particle, both CA domains constitute ... crystallography and ⁄ or NMR spectroscopy.The N-terminal domain of CA (NTD) and the C-ter-minal domain of CA (CTD) are small, globular and mainly helical. NTD contains a-helices 1–7 of CA, and is connected...

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: designing inhibitors of capsid assembly ppt

... Trp184 and Met185;HSQC, heteronuclear single quantum coherence; MA, matrix protein of HIV-1; NC, nucleocapsid protein; NTD, N-terminal domain of CA,comprising residues 1–145 of the intact protein; ... able to interact with CA and ⁄ or itsdomains, hampering HIV-1 assembly and infection.AbbreviationsCA, capsid protein of HIV-1 (p24); CAC1, a CTD-binding designed peptide; CAI, a CTD-binding phage ... formation of the peptide– protein and protein protein complexes.These NMR experiments also allowed the determina-tion of the CTD binding site. However, instead of usingthe dimeric wild-type CTD...

Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... 91–118.19 Hibi M, Lin A, Smeal T, Minden A & Karin M (1993)Identiﬁcation of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the c-Junactivation domain. Genes Dev ... cell line PC-9. To investigate the signaling routeby which AG1478 induced apoptosis, we examined the activation of c-JunN-terminal kinase (JNK) and mitogen-activated protein kinase p38 in AG1478-treated ... selec-tive protein kinase C inhibitor, Ro-31-8220, inhibitsmitogen-activated protein kinase phosphatase-1 (MKP-1) expression, induces c-Jun expression, and activatesJun N-terminal kinase. J Biol...

Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx

... neuroepithelialcells into astrocytes, by binding and hyper activatingthe promoter of glial ﬁbrillary acidic protein (GFAP). In parallel, BMP signals lead to enhanced expression of ID proteins, which in turn bind ... is initiated, resulting in theblockage of any kind of differentiation (Fig. 1).Taking these data from the culture system, it is inter-esting to look at studies on BMP signaling proteins in ... resulted in a strong growth inhibition of thestem cells but, fascinatingly, did not interfere with theirpluripotency, as judged by mRNA levels of Oct4,Nanog and Sox2. Inhibition of BMP signaling...

Tài liệu Báo cáo khoa học:Symmetric fluoro-substituted diol-based HIV protease inhibitors Ortho-fluorinated and meta-fluorinated P1/P1¢-benzyloxy side groups significantly improve the antiviral activity and preserve binding efficacyy docx

... gag and gag-pol precursor polyproteinsinto structural and f unctional proteins. The HIV protease has been shown to be essential in the production of mature and infectious virions [4,5], hence inhibition ... pharmacokinetics in rats, which led us toinvestigate the effect of ﬂuoro-substituted inhibitors on cellabsorption. The substitution of ﬂuorine for hydrogenintroduces a minor increase in molecu ... mgÆmL)1 protein in 50 mMMES, pH 6.5, con-taining 10 mMdithiothreitol and 1 mMEDTA, and 7 mMinhibitor in 10% (v/v) dimethyl sulfoxide. The reservoirsolution contained 50 mMMES, pH 5.5, and...

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