Tài liệu Báo cáo khoa học: Autophagy inhibits reactive oxygen species-mediated apoptosis via activating p38-nuclear factor-kappa B survival pathways in oridonin-treated murine fibrosarcoma L929 cells doc

... species-mediated < /b> apoptosis < /b> via < /b> activating < /b> p38-nuclear < /b> factor-kappa < /b> B survival pathways in oridonin-treated murine fibrosarcoma L929 cells Yan Cheng 1,2 , Feng Qiu 2 , Yuan-Chao Ye 1 , Zhao-Ming Guo 1 , Shin-Ichi ... time after oridonin administration. These results indicate that oridonin induced autophagy < /b> in L929 cells....

Ngày tải lên: 18/02/2014, 13:20

... been identified as insoluble proteins in many diseased tissues including amyloid fibrils [7,8]. We are investigating the role of amino acid side chain oxidation in amyloid assemblies by comparing ... entropy in binding and folding: general methods and application to helix formation. Proteins 20, 68–84. 12 Kay MS & Baldwin RL (1996) Packing interactions in the apomyglobin folding int...

Ngày tải lên: 19/02/2014, 05:20

... glucan synthesis. Abbreviations DP, degree of polymerization; GBd, glucan-binding domain; GS, glucan-binding domain-deficient glucosyltransferase-I; GSd, glucansucrase domain; GSGB, glucosyltransferase-I containing ... are described in Doc. S1. 13 C-NMR analysis To inhibit base-catalyzed reactions, the insoluble glucan was dissolved at 60 mgÆ mL )1 in 0.5 m NaOD in D 2 O con- taining 3....

Ngày tải lên: 14/02/2014, 22:20

... former effect being efficiently increased by a cathepsin B ⁄ cathepsin D pretreatment. The data show a novel processing pathway for internalized ETA, involving cathepsins B and D, resulting in the production ... [22]. The antibody against KX 5 KDEL also binds to undefined endosomal proteins distinct from ETA, both in control and in toxin-injected rats, whose levels have been shown to...

Ngày tải lên: 18/02/2014, 04:20

... hóa học b ng phổ hồng ngoại FTIR. Mẫu b t gỉ thép và gỉ thép đã được xử lý b ng chất biến đổi gỉ B- 05 trộn với b t KBr và nén thành viên với tỷ lệ 2%, được phân tích FTIR trong dải b ớc sóng ... Trong khi đó, trên b mặt gỉ thép đã được xử lý b ng chất B- 05 có cấu trúc mịn, đồng nhất, không b xốp rỗng (Hình 1 .b) . Như vậy b ớc đầu có thể thấy rằng sau khi được xử lý gỉ b mặt...

Ngày tải lên: 18/02/2014, 13:20

... proteins by western blotting analysis. b- Actin was used to normalize equal protein loading; Blot images represent one of three independent experiments. (C) MTP was determined using a JC-1 probe. ... osteoarthritic joints was increased com- pared with that in control joints. Changes in TRAIL and ubiquitin levels may be linked to the progression of in ammation and may be detected in th...

Ngày tải lên: 18/02/2014, 14:20

... were obtained in ChIP DNA, indicating that the binding of putative CERK-PPRE to PPARb is specific. Furthermore, the interaction between PPARb and CERK was confirmed by an EMSA using biotin- labeled ... of PPARb, which enhanced cell survival or inhibited cell death induced by serum starvation. Next, we investigated the participation of Cer in cell survival promoted by PPARb, by examining...

Ngày tải lên: 18/02/2014, 18:20

... Reser- voir Counting for application in online Local- ity Sensitive Hashing. This technique allows for significant savings in the streaming setting, allowing for maintaining a larger number of signatures, ... function, this may be justified in greater overall fidelity from the resultant increase in b. 1 A related point was made by Li et al. (2008) when dis- cussing stable random projec...

Ngày tải lên: 20/02/2014, 04:20

... treatment, cells were incubated in medium containing 50 lgÆmL )1 neutral red at 37 °C for 3 h, then fixed with 1% formaldehyde containing 1% CaCl 2 for 1 min. The dye incorporated into viable cells ... apoptosis < /b> in F9 cells using SB202190, a potent inhibitor of p38. As shown in Fig. 7A, although hydrogen peroxide-treatment induced apoptosis < /b> in F9 cells as describe...

Ngày tải lên: 21/02/2014, 03:20

... curcumin with microtubule inhibitors for tub- ulin binding. (A) Colchicine and podophyllotoxin weakly inhibited binding of curcumin to tubulin. Data represent mean ± SEM (n ¼ 4). (B) Binding of ... 30 min incubation at 25 °C by exciting the samples at 425 nm. Binding of curcumin to preformed tubulin– colchicine or tubulin–podophyllotoxin complex Tubulin (3 l m) was incubated in the absence...

Ngày tải lên: 19/02/2014, 05:20