Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... compilation ª 2009 FEBS An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori Naoki Okamoto 1 , Naoki Yamanaka 2, *, Honoo Satake 3 , ... secreted in a developmentally regulated manner [4]. ILPs are also found in insects [5,6]. Among these, bombyxins, a family of peptides produced by the brain...

Ngày tải lên: 18/02/2014, 13:20

... cells transfected with the HSS siRNA as compared with controls (Fig. 7C). This indicates that there was an impairment of the permeabilization transition pore (PTP) of the mitochondrial membrane and ... generation of an electro- chemical gradient across the IM forms the basis of the IM w m . During cell death, MP often increases, allow- ing for the release of solubl...

Ngày tải lên: 16/02/2014, 09:20

... vascu- lar tissue of cotyledons and mature leaves, whereas, in the flowers, the anthers, connectives and filaments and the base and tip of the stigmata were stained. Similar to BPM2, BPM3 and BPM5 promoter:GUS ... cotyledons and in the central cylinder of the root, but not in the lower parts of the hypocotyl (a). Rosette leaves were stained in the vascular tissue of...

Ngày tải lên: 18/02/2014, 06:20

... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurrence of a transition between 35° and 55 °C. PHI ... the first hypothesis and therefore a direct involvement of PHI in electron transfer. This conclusion is consistent with the very low degree of identity between the N...

Ngày tải lên: 21/02/2014, 00:20

... (ortho- logue of human RPE65) and retains all of the known key residues for the enzymatic activity of RPE65. Similar to the other RPE-specific RPE65, RPE65c was present in both the membrane and cytosolic ... spans the entire thickness of the inner retina. The Mu ¨ ller cell constitutes an ana- tomical link between the retinal neurones and supports their activities by...

Ngày tải lên: 14/02/2014, 14:20

... for 30 min, and the supernatant was decanted. The pellet was resuspended in 10 mL of supernatant, and then centrifuged at 20 000 g for 30 min. The supernatant was decanted, and 10 mL of 20 mm Tris ... this time, the expression of LF was induced in the supernatant by the addition of 20% xylose (Sigma-Aldrich) to a final concen- tration of 0.5%. The culture was grown for...

Ngày tải lên: 16/02/2014, 09:20

... equivalent to the Meis2a splice variant, and these two proteins share 74% identity and 80% similarity over their C-terminal domains. Comparison of the Meis2d isoform analyzed here with the public ... lacking the HD have been identified [38,39]. The Meis2e variant, which is truncated prior to the end of the first a-helix of the HD, has been suggested to act as a dominant ne...

Ngày tải lên: 16/02/2014, 15:20

... (stems I and II) flanked by regions of ssDNA [21]. The 5¢-arm of the enzyme binds the cleavable sequence via Watson–Crick base pairs and the 3¢-arm through formation of a DNA triplex. The catalytic ... the sequence is changed to 5¢-TCGG-3¢, which is the same as the bulge sequence of 8–17 variant DNAzyme. The stem and terminal loop (5¢-AGC-3¢) of 8–17 variant DNAzyme...

Ngày tải lên: 16/02/2014, 15:20

... handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as in the AFM surface representation of Fig. 2A. By comparison with the ... composition of the c ring from A. woodii The above structural analyses clearly assigned 22 transmembrane helices to the c ring of A. woodii.To unravel the c 1 and c 2...

Ngày tải lên: 18/02/2014, 17:20

... the depletion of the Myc tag only changes the size of the upper band, and that therefore the upper band repre- sents the ‘full-length’ s-DAPK-1. Two s-DAPK-1 deletion mutants, Flag-AO (Anky- rin ... determine the mechanism that underlies the effect of the tail on the membrane-blebbing-promoting ability of s-DAPK-1, the localization and half-lives of the full-...

Ngày tải lên: 18/02/2014, 17:20