Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx

... Research Center Borstel. Journal compilation ª 2008 FEBS 659 Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide Sabine Riekenberg1, Katja Farhat1, ... or TLR2 ⁄ 6 lipopeptide ligands and theTLR4 ⁄ MD2 ligand lipopolysaccharide, microarray analyses show onlymodulation of RGS1 and RGS2 among all the regulators of G-protein sig-nalling tested. ... modula-tion of RGS1 and RGS2 in BMDM after stimulationwith LP and LPS in more detail, because regulation of RGS1 and RGS2 after activation of different TLRmay modify the effects of G-protein signalling...

Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx

... 5-methyltetrahydrofolate-binding pocket of rat liver MetS. Atoms within 7 A˚ of the docked ligand are shown:hydrophobic amino acid residues are coloured bronze, and hydrophilic acid residues are coloured ... result of this approach indicated the orienta-tion of 5-methyltetrahydrofolate in the MetS activesite. Figure 5 shows the superimposed structures of DHPS and 5-methyltetrahydrofolate. The model of ... reactions of trans-sulfura-tion through the production of homocysteine, biologi-cal methylations of DNA, lipids and proteins, and polyamine biosynthesis through the production of methionine and S-AdoMet...

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... mediated by the ANT in maximallypolarized (forward mode of both ANT and ATPase) and maximally depolarized (reverse mode of bothANT and ATPase) mitochondria.Flux control coefﬁcients of ANT and F0F1-ATPsynthase ... difference of DWm (termedDelta phi) before and after the addition of ADP(2 mm) to WT and CYPD KO mitochondria, and cal-culated on the basis of steady-state titration data by catr and olgm. ... 1115Values and explanations of all parameters of Eqns(1,2) are taken from previous studies [22,27]. Ti and Diindicate free matrix ATP and ADP concentrations,respectively, whereas To and Doindicate...

Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx

... University of Science and Technology, Hunan, China3 College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, China4 School of Science, ... activation of endothelialcells and macrophages, as well as the differentiation and proliferation of VSMCs. Overexpression of KLF4induced expression of multiple anti-inﬂammatory and antithrombotic ... levels of KLF4were determined by western blot. (C) mRNA levels of hSR-BI weredetermined by real-time PCR. (D) Protein levels of hSR-BIwere determined by western blot. The relative values of all...

Tài liệu Báo cáo khóa học: Inactivation of copper-containing amine oxidases by turnover products doc

... cuvette closed by a Teﬂon stopper. The oxygen was consumed by mostsubstrates within the mixing time as revealed by theimmediate bleaching of the TPQ 500 nm band and by theappearance of absorption ... the absence of H2O2,a speciﬁc inactivating reaction, without formation of the350 nm band, was induced by some aldehydes, notablyputrescine. Some mechanisms of inactivation are proposed.Keywords: ... Sciences ‘A. Rossi Fanelli’ and C.N.R. Institute of Molecular Biology and Pathology,University of Rome ‘La Sapienza’, Rome, Italy;2Department of Biology, 3rd University of Rome, Rome, ItalyFor...

Tài liệu Báo cáo khóa học: Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reesei ) Cel7B catalytic module pptx

... observation of species of increasing mass spaced by 162 Da reﬂects the presence of glycoforms with an increasing number of hexose (probablymannose) units. Phosphorylation is indicated by the pres-ence ... results of detailed carbohydrate analysis (Fig. 6) and peptide MS experiments (Fig. 3A) deﬁne the glycoformstructure and the sites of glycan attachment in the presentenzyme preparation. TOF MS and ... due to O-linked glycosylation of theprotein (see below). The results are summarized in Table 2.Identiﬁcation of N-glycosylation sitesA series of detailed TOF MS and CID MS/MS experimentswere...

Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... Inhibition of the ATPase activity was then explained by a slowingdown of the formation of active complex in solution. On theother hand, the acceleration of the sliding velocity (and thelower number of ... proteins as revealed by the absence of cosedimentation of T800 with myosin duringa low speed centrifugation experiment (data not shown), and by the absence of interaction between titin and HMM-coated ... was not affected by ATP and/ or by CaCl2but it was decreased by elevating ionic strengthfrom 80 to 180 mMwith average values of 62.3 ± 2.2% and 45.1 ± 3.5%, respectively (compare d etailed values...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... ÀðkA=k0Þ=½Að1Þwhere kA and k0 are the inactivation rate constantseither in the presence or in the absence of variousconcentrations of a ligand A, k2 and k1 are therate constants of the inhibition of the ... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade.AcknowledgementsThanks are due to Prof G.L. Sottocasa and Dr Anto-nella Bandiera (University of Trieste) ... in the text and in Fig. 5B. n, Number of [A] tested; k2⁄ k1, the value of the intercept in the Scrutton and Utter plot, where k2 and k1 are the rate constants of the inhibition of either...

Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx

... in its1Dgstate;1O2)isgenerated by a number of enzymatic and chemical reactions, by UV exposure, and by visible light in the presence of anumber of exogenous or endogenous cellular sensitisers.1O2generation ... inactivation of GAPDH and GR occurs via oxidation of nonactive siteresidues, that bring about loss of functional integrity, thestoichiometry of inactivation ( i.e. the loss of two molecules of thiol ... 1925Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attackPhilip E. Morgan1, Roger T. Dean2 and Michael J. Davies11EPR and 2Cell...

Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx

... morphologically and biochemically distinct modes, necrosis and apoptosis [1].Necrosis is characterized by swelling of organelles and cells,followed by lysis of the plasma membrane and randomDNA ... (Hsps) are a set of highly conservedproteins that are induced in response to physiological and environmental stress, and are classiﬁed into several familieson the basis of their apparent molecular ... activation of p38is an essential step for induction of apoptosis by hydrogenperoxide, and Hsp105a is suggested to enhance the oxida-Fig. 6. Eﬀects of Hsp105a on activation of JNK and p38 by oxidative...

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