... Second, the building block of the ligand having the strongestcontact with the protein shows the most intense NMRsignals, enabling mapping of the ligand’s binding epi-tope. Finally, and most ... could indicate that the corre-sponding hydroxyl groups are involved in proteinbinding. The results for the linebroadening measurements of protons H1a in the alpha and beta configurations,aHa1 and ... Nevertheless, information concerning the relativebinding contribution can be obtained by comparing the intensity of the signals in this region with that of protons H2 and H6. By comparison of the...