Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: probes of hydrogen tunnelling pptx

... FEBS MINIREVIEW Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain Dennis J. Stuehr, Jesu ´ s Tejero and Mohammad M. Haque Department of ... an accurate measure of the k off parameter in CaM-bound eNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational cha...

Ngày tải lên: 18/02/2014, 11:20

... 3941 MINIREVIEW Structural and mechanistic aspects of flavoproteins: probes of hydrogen tunnelling Sam Hay, Christopher R. Pudney and Nigel S. Scrutton Manchester Interdisciplinary Biocentre and Faculty of ... (1989) Enzymatic-synthesis of 4R-H-2NAD(P)H and 4S-H-2NAD(P)H and determination of the stereo- specificity of 7-alpha-hydroxysteroid and 12-alpha-h...

Ngày tải lên: 18/02/2014, 11:20

... and pyridine nucleotides is highly dependent on the approach and colinear orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of ... Bradley LH & Swenson RP (1999) Role of glutamate- 59 hydrogen bonded to N(3)H of the flavin mono- nucleotide cofactor in the modulation of the redox potentials of the Clos...

Ngày tải lên: 18/02/2014, 11:20

... base of bound GMP is stacked between Val143 and Phe194. N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with both O6 and N7 ... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filt...

Ngày tải lên: 15/02/2014, 01:20

... R52 and D190 and between R114 and D99 (cut off value 4.5 A ˚ ) – and 33 strong hydrogen bonds (cut off value 3 A ˚ ) across the dimeric interface. The thermophilic SurEs have a larger number of ... A ˚ ), with OD1 and OD2 of D8 (2.82, 3.41 A ˚ ), with ND2 and OD1 of N96 (2.92, 3.02 A ˚ ), with OG1 of T106 (3.03 A ˚ ), with N of G105 (3.05 A ˚ ) and G40 (3.05 A ˚ ). A...

Ngày tải lên: 18/02/2014, 14:20

... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... side chain of Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain...

Ngày tải lên: 18/02/2014, 16:20

... ionic strength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD) seems to favor formation of dodecahedra over isolated pentamers. Previous structural analysis of the ... is formed by an insertion of  230 residues (residues 133–367) between strands D and E and a smaller insertion of  50 residues (residues 404–458) between strands...

Ngày tải lên: 19/02/2014, 06:20

... mRNA and protein levels of key pro- fibrotic proteins like PDGFR-a and -b, TGFb1, and TIMP-1 and -2 in the liver of transgenic mice. PDGF-C treatment increased both mRNA and protein levels of the ... model of the PDGF-C growth factor domain indicates the disulfide bridges in PDGF-C to consist of Cys250 and 294, Cys280 and 335, and Cys287 and 337, and the inter- mon...

Ngày tải lên: 19/02/2014, 07:20

... specifics of each task. One contribution of this paper is a thorough analysis of the differences and similarities of mul- tilingual patent data along the dimensions of tex- tual structure and topic. ... devel- oped several different formalizations of the cen- tral idea of trading off optimality of parameter vectors for each task-specific model and close- ness of these mo...

Ngày tải lên: 22/02/2014, 03:20

... with a high proportion of phosphory- lated receptors having Grb14 bound to them. Structural studies of the kinase domain of the IR in complex with the PIR–BPS of Grb14 and of the Grb14 SH2 domain ... receptor and Grb7. A role for two of its binding domains, PIR and SH2. Oncogene 19, 2052– 2059. 36 Daly RJ, Sanderson GM, Janes PW & Sutherland RL (1996) Cloning and cha...

Ngày tải lên: 18/02/2014, 18:20