Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf

... 3959–3974 ª 2009 The Authors Journal compilation ª 2009 FEBS MINIREVIEW Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain Dennis ... the interaction between the FMN subdomain and the FNR subdomain, although some may also influence the rate of electron import into the FMN...

Ngày tải lên: 18/02/2014, 11:20

... Klinman and cowork- ers to study the viscosity dependence of the rate of hydride transfer in GO [70,71]. These authors studied various glycoforms of the enzyme (varying in the extent of glycosylation) ... deutera- tion on the observed rate and KIE of an H transfer reaction. If k D is underestimated then so too will be DDH à and the effect of f D on the appa...

Ngày tải lên: 18/02/2014, 11:20

... Hydride transfer in systems involving flavins and pyridine nucleotides is highly dependent on the approach and colinear orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the ... [48]. Interaction of FNR with the NADP + coenzyme and the hydride transfer event Once the FAD cofactor of FNR has accepted two electrons, they have to be...

Ngày tải lên: 18/02/2014, 11:20

... II. The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two ... stacked between Val143 and Phe194. N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with bot...

Ngày tải lên: 15/02/2014, 01:20

... to the side chain of Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and ... to the three lysines (Fig. 3E). There is no water molecule fix- ing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits...

Ngày tải lên: 18/02/2014, 16:20

... 4339 containing the RGD motif, is located at the top of the distal domain within the first insertion, and faces the solvent-exposed exterior of the particle. As in the hAd2pb structures, even the shorter ... (left) and rainbow (right). The N-terminus and C-terminus are labeled in both monomers. On the left, the b-strands of the jellyroll domain are label...

Ngày tải lên: 19/02/2014, 06:20

... indicate the starting and ending residues of the secondary structural elements. (C) The domain- swapped dimer of St SurE. The A and B subunits are shown in green and blue, respectively. The darker and ... as in archaic and thermophilic homo- logues. The St SurE monomer consists of 13 b-strands, six a-helices and three 3 10 -helices. The core of the pro-...

Ngày tải lên: 18/02/2014, 14:20

... removal of the CUB domains and thereby activation of the growth factor domains. PDGF-C and -D contain both the CUB and growth factor domains when they are secreted and pro- teolytic cleavage is therefore ... model of the PDGF-C growth factor domain indicates the disulfide bridges in PDGF-C to consist of Cys250 and 294, Cys280 and 335, and Cys287 and 337, an...

Ngày tải lên: 19/02/2014, 07:20

... use the A-distance measure of Kifer et al. (2004). If A is the set of events on which the word distributions of two corpora are defined, then the A-distance is the supremum of the difference of probabilities ... Proceed- ings of the Human Language Technology Confer- ence and the 3rd Meeting of the North American Chapter of the Association for Computational...

Ngày tải lên: 22/02/2014, 03:20

... catalyses either the hydrolysis or the transfer of phosphate esterified in the 5¢ position of 6-hydroxypu- rine monophosphate nucleosides [1]. The transfer of phosphate can lead to phosphorylation of inosine, guanosine ... disappearance of the heavier inactive peak. Furthermore, FPLC analysis of the recombinant wild-type cN-II showed the presence of the tetram...

Ngày tải lên: 15/02/2014, 01:20