Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf

... compilation ª 2009 FEBS 3059 The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro -retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo Jan P. Schuchardt 1 , ... Identification of 9,13-dicis -retinoic acid as a major plasma metabolite of 9-cis -retinoic acid and limited transfer of 9-cis -retinoic acid an...

Ngày tải lên: 18/02/2014, 08:20

... which revealed that the BMPR-IA binding site of the related BMP-7 largely coincides with the noggin-binding area [32,42]. Thus, the proBMP-2 binding to noggin detected here indirectly confirms the interaction ... specifically to the ECD of BMPR-IA. Furthermore, the results indicate that both proteins interact with the same epitope on the ECD because proBMP-2 binding can be i...

Ngày tải lên: 18/02/2014, 06:20

... of the res- idues involved in phosphate and calcium binding are conserved in the PhyH-DII sequence, whereas only one phosphate-binding residue is conserved in the PhyH-DI sequence (Fig. S1). There ... (Fig. 3A,B). The presence of Ca 2+ increased the thermal stability of PhyH and PhyH-DII. After incubation at 60 °C for 30 min, both enzymes retained > 70% of their initi...

Ngày tải lên: 14/02/2014, 15:20

... be involved in various signalling pathways, including blue light signalling [10] and auxin signalling [11–13]. The majority of group VIII AGC kinases are phosphorylated and activated by another AGC ... co-immunoprecipitation experiments. We then examined how both proteins interact with MPK3 and MPK6 proteins. Results AGC kinases interact with PTI1 kinases in vitro To iso...

Ngày tải lên: 14/02/2014, 19:20

... E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin- associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48. Furthermore, A-Myb and B-Myb were present in immunoprecipitations ... confirmed by Lin-54 bind- ing to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86]. ChIP experiments on the Cdc2 gene also provide...

Ngày tải lên: 16/02/2014, 09:20

... [12]. Maintaining the balance between cell survival and apoptosis is critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in either Keywords apoptosis; ... produced (Fig. 1D, lane 2). In the presence of lactacystin, a sig- nificant increase in the amount of tBid–GFP present in the extract, both in con...

Ngày tải lên: 16/02/2014, 09:20

... were 231 min for the b-globin PAI)2 wild-type transcript, 204 min f or the b-globin ARE I-MUT , 193 min for the b-globin ARE III-MUT and 224 min for the b-globin ARE I+III-MUT . These experiments ... or by northern analysis (10 lgof total RNA) using the intron spanning 32 P-labelled globin and GAPDH probes described above. The b-globin and GAPDH mRNA band intensities wer...

Ngày tải lên: 16/02/2014, 09:20

... specific interactions between cis-acting elements mainly localized in the UTRs of the transcript and the trans-acting factors (RNA-binding proteins and noncoding regulatory RNAs) that bind to these ... post-transcriptional regulation. Therefore, the results obtained in the pres- ent study support the recent findings showing that members of the SR proteins family, including...

Ngày tải lên: 16/02/2014, 15:20

... expressed in vitro in the presence of either Zn(II) or Cd(II). Metal binding stoichiometries and affinities were determined by ESI-MS and NMR, respectively. Both isoforms had equal zinc binding ability, ... to the wild-type strain. Both CeMT-1 and CeMT-2 are important in maintaining physiological zinc levels Under control (non-metal-supplemented) conditions in the wil...

Ngày tải lên: 16/02/2014, 15:20

... (Fig. 1). The MCO sequence motif pattern, which contains the four elements that together form the copper-binding sites in the protein, is conserved in McoP, including a Met corresponding to the axial ... rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. Supporting in...

Ngày tải lên: 18/02/2014, 04:20