Tài liệu Báo cáo khoa học: The cartilage-speciﬁc transcription factor Sox9 regulates AP-2e expression in chondrocytes pptx

Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt

... replace the tri-glycine segment in the envelope targetingsequence. Thus, mtHsp70-like protein may not beinvolved in the polyglycine-mediated envelope targeting. In order to test the second hypothesis, ... that the most C-terminal tri-glycinewithin the polyglycine stretch is important for correctlytargeting the protein to the outer membrane. Interest-ingly, replacements of the tri-glycine with ... excluded at thispoint. In the ﬁrst scenario, there may be a proteina-ceous component either in the chloroplast envelopeintermembrane space or in the inner membrane thatbinds to the ﬂexible, small,...

Tài liệu Báo cáo khoa học: "The Utility of a Graphical Representation of Discourse Structure in Spoken Dialogue Systems" ppt

... result, the instruction did not match the initial essay quality. Nonetheless, in the open-question interviews, many users indicated using the NM as a reference while updating their essay. In addition ... perception of the system in terms of their ability to concen-trate on the instruction (Q3), in terms of their incli-nation to reuse the system (Q6) and in terms of the system’s matching of their expectations ... holding them. Then we will look at the motion of the person and his keys after he lets go of them. Let's begin by looking at the motion of the man andhis keys while he is holding them....

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... cobalamins (2.9–5.1°)[9,10].Figure 4C indicates the comparison of the position of the a-acetamide side chain of pyrrole ring A of the corrin ring in the glycerol dehydratase-bound cobalamin (Fig. ... bulk of water by the corrin ring of cobalamin. Figure 4B shows the structurearound the enzyme-bound cobalamin. The cobalaminmolecule is bound between the a and b subunits in the so-called Ôbase-onÕ ... hydrogen-bonded to the amide oxygen of the g-acetamide side chain of the corrin ring in glyceroldehydratase (red dotted line in Fig. 4B). In diol dehydra-tase, the corresponding residue is Prob155...

Tài liệu Báo cáo khoa học: "The Structure of User-Adviser Dialogues: Is there Method in their Madness?" pdf

... attempt to find evidence linking the dialogue structure to the task structure. The main point is that the structure of the intentions conveyed in the discourse should mirror to some extent the task ... at the beginning of the subdialogues than later in the subdialogues. On the other hand, pronominal noun phrases are used to refer to entities currently in focus. In other words, pronominal ... to the current subdialogue N. Thus, the node N-1 immediately dominates N, the node N-2 dominates N-I, and so on. The nodes subordinate to each of the nodes dominating N are indexed beginning...

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... We focuson the function and relationship of the two singledomains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain BPPswould improve the catalytic ... of the res-idues involved in phosphate and calcium binding areconserved in the PhyH-DII sequence, whereas onlyone phosphate-binding residue is conserved in the PhyH-DI sequence (Fig. S1). There ... they can increase the amount of available phosphate by interacting together. Additionally,fusing PhyH-DI to a single-domain phytase appears to be an efﬁcient wayto improve the activity of the...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... alone. The kinase-deﬁcient mutant,OXI1K45R, in which the lysine residue of the ATP bind-ing domain is mutated to arginine, still interacted withPTI1-4. These data indicate that the kinase ... of the input was loaded on to the geland represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeated twice usingrecombinant proteins prepared independently ... preincubat-ed in kinase buffer with or without HIS-PTI1-4 for the indicatedtime points. The mixes were then incubated with [c-32P]-ATP andMBP (10 l g) for 30 min. The top panel shows the kinase...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... conﬁrmed by Lin-54 bind-ing to the CHR, cooperating with E2F4 binding to the CDE in EMSAs in vitro [86].ChIP experiments on the Cdc2 gene also providedinsights into the association of other cell ... proteinswith the promoter. E2F4 binding during the cell cyclecoincides with the binding of p107 or p130 [61,85].Another E2F site distal to the E2F ⁄ CDE acts as anactivating element binding ... [87].However, the limitation of ChIP analyses is that the exact binding location is not identiﬁed in the experi-ments. Therefore, it is not clear whether the CDE ⁄ CHRis involved in binding these factors.Consistent...

Tài liệu Báo cáo khoa học: The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid ppt

... critical in the maintenance of a healthy organism, and tipping the equilibrium in one or another direction results in eitherKeywordsapoptosis; Bid; c-Jun N-terminal kinase;epidermal growth factor; ... through the mitochondrial pathway. The key event in the mito-chondrial pathway is the release of proapoptotic fac-tors from the mitochondrial intermembrane space into the cytosol, resulting in the ... protein of the Bcl-2family that is crucial for death receptor-mediatedapoptosis in many cell systems [16,17]. The BH3domain-only proteins are a subfamily of the Bcl-2family involved in the initiation...

Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... were 231 min for the b-globinPAI)2wild-type transcript, 204 min f or the b-globinARE I-MUT,193 min for the b-globinARE III-MUTand 224 minfor the b-globinARE I+III-MUT. These experiments ... investigate the means by which these ARE elementscooperate in modulating PAI-2 mRNA stability,REMSA analyses were performed to determine the role of the ARE II and ARE IV sites in the binding ... plasmidpTETGLOPAI)2using primers SJS137 and SJS138, andcloned into the BglII site in the b-globin 3¢ UTR in pTET-GLO, to generate pTETGLOEXT.ARE. The sequences of the primers used in the present study...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... nuclear in normal cells, the splicing factor ASF ⁄ SF2 relocalized into the cytoplasm in response to stress and accumulated in bona ﬁdeSGs. Furthermore, the results obtained in the presentstudy indicate ... post-transcriptionalregulation. Therefore, the results obtained in the pres-ent study support the recent ﬁndings showing thatmembers of the SR proteins family, includingASF ⁄ SF2 and SRp20, have important roles in the cytoplasmic ... SF2 toinduce the destabilization of the mRNA encodingPKCI-related protein by direct binding to a purine-richsequence present in PKCI-r mRNA 3¢ UTR [15]. On the other hand, other studies indicated...

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