Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... Journal 276 (2009) 238 1–2 390 ª 2009 The Authors Journal compilation ª 2009 FEBS Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins Aldino ... interactions established in the active site is also of relevance. The active site: structural and electrostatic basis of HtA functio...

Ngày tải lên: 18/02/2014, 08:20

... structure and potential surface The characteristic NOE connectivities, reported in Fig. 3A, and the analysis of the / and w angle distribution allowed the identification of the type and boundaries ... b, myotoxins and a- neurotoxins [34,35]. The presence of a positive band at approximately 195 nm and of a negative one in the range 21 0–2 15 nm, usuall...

Ngày tải lên: 20/02/2014, 11:20

... of the Ramachandran plot shows that the dihedral angles / and w for the secondary structure elements are all found in the most favored or the additionally allowed region. A selected example of ... analysis NMR data were analysed and processed with the computer programs XWINNMR , AURELIA [22], and AUREMOL [23] (Bruker, Karlsruhe, Germany). In the final part of the...

Ngày tải lên: 21/02/2014, 00:20

... to GH), and by the Natural Sciences and Engineering Research Council of Canada (to GH). DSL was the recipient of an Ontario Graduate Scholarship. The authors are grateful to Dr Nam-Chiang Wang of the Peptide ... high relaxation with 5-DSA have relatively lower relaxation because of the presence of Fe 3+ . The apparent fast relaxation of V7¢ in the presence of b...

Ngày tải lên: 19/02/2014, 07:20

... bound to the mAb Fab131 Andreas G. Tzakos 1 , Alexandre M. J. J. Bonvin 2 , Anasstasios Troganis 3 , Paul Cordopatis 4 , Mario L. Amzel 5 , Ioannis P. Gerothanassis 1 and Nico A. J. van Nuland 2 1 Department ... interactions, there are three general approaches that can be utilized to extract structural information [4]: a peptide (ligand)-based approach, a receptor-based approach, an...

Ngày tải lên: 20/02/2014, 23:20

... nystatin A1 and rimocidin) and two heptaenes (candidin and vacidin A) have been calculated using geometric restraints derived from 1 H-NMR data and random searches of their conformational space. ... ergosterol, causing a major disorganization of the membrane structure [5] leading to the leakage of cellular materials and in turn the cellular death. Depending on thei...

Ngày tải lên: 21/02/2014, 03:20

... 2295 Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases Juha P. Kallio 1 , Chiara Gasparetti 2 , Martina Andberg 2 , Harry Boer 2 , ... weak homodimer, as observed for MaL, that may determine the properties of these asco-laccases at high protein concentrations. Database Structural data are available in the Pr...

Ngày tải lên: 14/02/2014, 18:20

... atoms) and r j is the standard deviation of B factors. The normalized B factors have a zero mean and unit variance. All atoms that satisfy B z ‡ 4 are treated as outliers and discarded. After ... binding sites, referred to as the major and minor binding sites [1]. These sites are located in the concave face of the protein near regions of invariant Trp and Asn array...

Ngày tải lên: 14/02/2014, 19:20

... Mn-bound (magenta) and Fe-bound (cyan) structures are superimposed, and the residues around the active site are shown via stick models. The red, magenta and cyan balls represent the active site water ... octahedral coordination around the metal cofactor. The absence of an anomalous Fourier map demon- strated that the active site in apo-ApeSOD did not contain...

Ngày tải lên: 14/02/2014, 22:20

... phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two domains [4,5]. HAPs can initi- ate ... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1...

Ngày tải lên: 16/02/2014, 09:20