Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx

... Prion protein library of recombinant constructs for structural biology Simone Hornemann*,à, Barbara Christenà, Christine von Schroetter, Daniel R. Pe´rez and KurtWu¨thrichInstitute of ... available for general use, and are distributed free of charge to not -for- proﬁtinstitutions.AbbreviationshPrP, human prion protein; mPrP, mouse prion protein; PrP, prion protein; PrPC, cellular form ... FEBSTable 1. List of plasmids encoding the sequence of the mature cellular form of the prion protein from a variety of species and truncatedvariants thereof, and of human doppel. The protein accession...

Tài liệu Báo cáo khoa học: "DiMLex: A lexicon of discourse markers for text generation and understanding" docx

... positions, whereas the positions of others are fixed. The linear order of the conjuncts is fixed for some markers and flexible for others; this is independent of the aforementioned two features. ... techniques. For tasks of this kind, DiMLex can supply the set of cue words to be looked for and support the initial disambiguation of cues in the text. Depending on the depth of the syntactic ... detailed studies of individ- ual groups of markers, such as [Vander Linden, Martin 1995] for PURPOSE markers. Besides, the Linguistics literature offers fine-grained analyses of individual markers,...

Tài liệu Báo cáo khoa học: "Domain-transcending mappings in a system for metaphorical reasoning" docx

... first place.Our principle of query-directed reasoning for metaphor understanding strongly supports the use of VNMAs. The kind of information VNMAsconvey will form a part of the initial query, as ... anger.Set-hood VNMA: If entities of a certain type Sin the source map to entities of type T in the tar-get, then a set of entities of type S in the sourcemaps to a set of entities of type T in the target.Set-Size ... Levels of goodness,importance or other types of value assigned bythe understander to states of affairs in the sourcedomain map identically to levels of goodness,etc., of their mappee states of...

Tài liệu Báo cáo khoa học: "Predicting the ﬂuency of text with shallow structural features: case studies of machine translation and human-written text" doc

... Proceedings of the 12th Conference of the European Chapter of the ACL, pages 139–147,Athens, Greece, 30 March – 3 April 2009.c2009 Association for Computational LinguisticsPredicting the ﬂuency of ... be used for gen-eral predictions of ﬂuency and readability. For our experiments we use the evaluations of Chinese to English translations distributed byLDC (catalog number LDC2003T17), for whichboth ... done in thecontext of machine translation for preliminary in-vestigations of ﬂuency. Nevertheless, our ﬁndingsare also potentially beneﬁcial for sentence-levelevaluation of machine translation.2...

Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

... cloning of the chitinase and CBP,as the N-terminus of the latter protein should be free of non-native amino acids after removal of the afﬁnitytag (because amino acid one of the mature protein ... represent 0% binding). For further veriﬁcation of binding, protein bound to thesubstrate was analysed by SDS-PAGE after removal of nonspeciﬁcally bound protein by washing with 1.5 mL of 50 mm Tris ⁄ ... in abundance by the structural biopoly-mers of plants (cellulose and hemicellulose) and is animportant source of energy for a variety of organisms.The primary degraders of chitin are microorganismsthat...

Tài liệu Báo cáo khoa học: Multisite protein phosphorylation – from molecular mechanisms to kinetic models pdf

... phosphorylation of the protein Sld2 by thecyclin-CDK complex during the S phase of the cellcycle [20,109], leading to formation of an activatorcomplex outside the origins and (b) a series of protein recruitments ... concentration of thekinase is much smaller than that of the target protein [28–30,82,83]. In this case, target proteins of variousphosphorylation states compete for the kinase (or,equally, for the ... the phosphorylation state of the protein. In the example shown, phosphoryla-tion of each site increases the probability of a closed conformation with a higher afﬁnity for the kinase, which accelerates...

Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc

... sequences of  40 amino acids found in many proteins involvedin protein protein interactions. The WD repeatdomain is the region of SCAP that forms a complexwith the C-terminal domain of SREBPs ... cascade of ubiquitintransfer reactions. The ubiquitin ligase (E3) required for the ﬁnal reaction of SREBP ubiquitination is acomplex consisting of Skp1, Cul1, Rbx1, and one of afamily of F-box proteins, ... number of proteins modiﬁedby SUMO, it has become obvious that the effects of SUMO conjugation are diverse and largely depend onthe function of the protein targeted for sumoylation.Sumoylation of...

Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

... thetype of anion in solution [17,18].Because structural information on partially foldedforms is important for a deeper understanding of thefolding mechanism(s) and the factors affecting protein stabilization, ... of sulfate. Therefore, the kinetic progresscurve of Fig. 9B is expected to refer: (a) for the fastphase, to the sulfate-induced destabilization of the HSacid-denatured form, with further formation ... 2006)doi:10.1111/j.1742-4658.2006.05527.x Structural information on partially folded forms is important for a deeperunderstanding of the folding mechanism(s) and the factors affecting protein stabilization. The...

Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

... Design and synthesis of a potentcyclic analogue of the myelin basic protein epitopeMBP72-85: importance of the Ala81 carboxyl groupand of a cyclic conformation for induction of experi-mental ... (CNS),and major variants of the illness are considered to beprimarily autoimmune in nature [1]. The 18.5 kDaisoform of myelin basic protein (MBP) is one of themost abundant proteins in CNS myelin; ... Martenson RE (1995) Con-formation of a tetradecapeptide epitope of myelin basic protein. Eur J Biochem 231, 659–666.34 Dyson HJ & Wright PE (2004) Unfolded proteins and protein folding studied...

Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc

... (enzymeI of the PTS), the ﬁrst protein in the cascade of proteinsforming the PTS, to HPr, the histidine-phosphocarrier protein. HPr is the smallest protein in the protein cascade of the PTS ... Measuring the conformationalstability of a protein. In Protein Structure (Creighton, T.E., ed.),2nd edn, pp. 253–259. Oxford University Press, Oxford.35. Privalov, P.L. (1979) Stability of proteins: ... increase in the number of urea protein inter-actions. For concentrations < 2.0Mdestabilization of the nativestate of the protein was not enough to detect a signiﬁcant structural unfolding...

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