Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx

... Prion protein library of recombinant constructs for structural biology Simone Hornemann* , à, Barbara Christenà, Christine von Schroetter, Daniel R. Pe ´ rez and Kurt Wu ¨ thrich Institute of ... available for general use, and are distributed free of charge to not -for- profit institutions. Abbreviations hPrP, human prion protein; mPrP, mouse prion protein; PrP,...

Ngày tải lên: 18/02/2014, 08:20

... positions, whereas the positions of others are fixed. The linear order of the conjuncts is fixed for some markers and flexible for others; this is independent of the aforementioned two features. ... techniques. For tasks of this kind, DiMLex can supply the set of cue words to be looked for and support the initial disambiguation of cues in the text. Depending on the d...

Ngày tải lên: 20/02/2014, 18:20

... first place. Our principle of query-directed reasoning for metaphor understanding strongly supports the use of VNMAs. The kind of information VNMAs convey will form a part of the initial query, as ... anger. Set-hood VNMA: If entities of a certain type S in the source map to entities of type T in the tar- get, then a set of entities of type S in the source maps to a set of...

Ngày tải lên: 22/02/2014, 02:20

... Proceedings of the 12th Conference of the European Chapter of the ACL, pages 139–147, Athens, Greece, 30 March – 3 April 2009. c 2009 Association for Computational Linguistics Predicting the fluency of ... be used for gen- eral predictions of fluency and readability. For our experiments we use the evaluations of Chinese to English translations distributed by LDC (catalog numb...

Ngày tải lên: 22/02/2014, 02:20

... cloning of the chitinase and CBP, as the N-terminus of the latter protein should be free of non-native amino acids after removal of the affinity tag (because amino acid one of the mature protein ... represent 0% binding). For further verification of binding, protein bound to the substrate was analysed by SDS-PAGE after removal of nonspecifically bound protein by washing w...

Ngày tải lên: 18/02/2014, 08:20

... phosphorylation of the protein Sld2 by the cyclin-CDK complex during the S phase of the cell cycle [20,109], leading to formation of an activator complex outside the origins and (b) a series of protein recruitments ... concentration of the kinase is much smaller than that of the target protein [28–30,82,83]. In this case, target proteins of various phosphorylation states c...

Ngày tải lên: 18/02/2014, 08:20

... sequences of  40 amino acids found in many proteins involved in protein protein interactions. The WD repeat domain is the region of SCAP that forms a complex with the C-terminal domain of SREBPs ... cascade of ubiquitin transfer reactions. The ubiquitin ligase (E3) required for the final reaction of SREBP ubiquitination is a complex consisting of Skp1, Cul1, Rbx1, and one of...

Ngày tải lên: 18/02/2014, 13:20

... the type of anion in solution [17,18]. Because structural information on partially folded forms is important for a deeper understanding of the folding mechanism(s) and the factors affecting protein stabilization, ... of sulfate. Therefore, the kinetic progress curve of Fig. 9B is expected to refer: (a) for the fast phase, to the sulfate-induced destabilization of the HS acid...

Ngày tải lên: 19/02/2014, 05:20

... Design and synthesis of a potent cyclic analogue of the myelin basic protein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation for induction of experi- mental ... (CNS), and major variants of the illness are considered to be primarily autoimmune in nature [1]. The 18.5 kDa isoform of myelin basic protein (MBP) is one of the most abunda...

Ngày tải lên: 19/02/2014, 07:20

... (enzyme I of the PTS), the first protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein. HPr is the smallest protein in the protein cascade of the PTS ... Measuring the conformational stability of a protein. In Protein Structure (Creighton, T.E., ed.), 2nd edn, pp. 253–259. Oxford University Press, Oxford. 35. Privalov, P.L. (1979) St...

Ngày tải lên: 19/02/2014, 12:20