Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... behav-ior by presenting the pulse of Src activity in the plane of the inactive and active fractions, siand s1. If the duration of the stimulus exceeds the critical value, the trajectory in the ... lipo-somes. The Src cycle can be started by the addition of relevant phosphatases (or other Src activators, such as the SH2/SH3-ligands) [38] to activate step 1, followed by the addition of Csk and ... of the period and amplitude of Src oscillations by the activities of the activatory phosphatase RPTP and inhibitorykinase Csk. Dependence of the oscillation amplitude (A) and period(B) on the...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... the peptide is bound in a concentrated mannerat the top of the column, or, if bound to the resinprior to packing the column, the salt gradient concen-trates the peptide on its way out of the ... recom-binant Ab have also been made by several othergroups, but most of these have relied on the generation of Ab fusions. Perhaps the best of these was reported by Lee et al. using a system in ... [41]. The use of thrombin signiﬁcantlyincreases the cost of the puriﬁcation, and the require-ment for HPLC increases the length and complexity of the puriﬁcation procedure. Moreover, as with the studies...

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... expect that the relativeexpression level of the GFP-tagged proteins is indicative of that of the equivalent untagged proteins.Lipid anchoring of C-Vrp1p bypasses the requirement for the LBD for ... 5A,B). Loss of the LBD abolished the ability of C-Vrp1p364)817torestore growth of vrp1D cells at elevated temperature.We next assessed the importance of the LBD for the ability of C-Vrp1p364)817to ... comprise the LBD of Vrp1p[20,27–29]. We therefore tested if the LBD is importantfor various Vrp1p-dependent functions (Fig. 5A–C).To examine whether deletion of the LBD abolishes the function of...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... was the same sizeas that of the translational product by isoform 2mRNA (Fig. 4B). The product by isoform 1 mRNAwas smaller than the isoform 2 product. The multiplebands may be explained by ... encoded another type of KLK11, isoform 2,despite the presence of exon 1c. The secretory path-ways of KLK11 isoforms appeared to differ, althoughboth isoforms were secreted from cells. Hence, the ... part of isoform 2 mRNA. The thirdexon, exon 1c, extended from +1379 to +1454. The noncoding exons of exons 1a and 1c contributed to the 5¢ nontranslated region of isoform 1 mRNA in whichthe...

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... hasFig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the ﬁgure) and of 4¢-phosphopantothenoylcysteine (+ ... via the phospho-peptide-like compound 4¢-phosphopantothenoylcysteine. The synthesis of 4¢-phosphopantothenoylcysteine is cata-lyzed by the C-terminal CoaB domain of Dfp and occurs via the acyl-cytidylate ... to be involved in the second half reaction of the (R)-4¢-phospho-N-pantothenoylcysteine synthetase. The proposed intermediate of the (R)-4¢-phospho-N-panto-thenoylcysteine synthetase reaction,...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... decreases upon the binding of Ca2+. The change in the ratio of F340 ⁄ F380 determined the change in Ca2+concentrationinside the cell.AcknowledgementsThis work was supported by The Norwegian ... mediates activation of Ras subsequent to the stimulation of Gi- and Gq-coupled receptors [8,9]. The main mechanism for the activation of Ras-GRF1 is the binding of Ca2+⁄ CaM to the N-terminalIQ ... truncatedforms is not known as they are missing the Ca2+⁄CaM -binding IQ domain that is involved in the activa-tion of Ras-GRF1.Stimulation of all the splice variants of the Gs-coupledserotonin...

Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx

... the scale of the Bard’s genera-tivity, and the quality of its insights.5 Empirical Evaluation The vastness of the web, captured in the large-scale sample that is the Google ngrams, means the Jigsaw ... acquiredfrom the Web for our vocabulary of stereotypes(this point relates to the aptness of the similes sug-gested by the Bard); and second, that the adjectivesconnected by the SlipNet really ... compare the predictions made in each caseto the actual pleasantness scores provided by Whissell’s dictionary, and thereby assess the qual-ity of the knowledge used to make the predictions.In the...

Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

... (HSPG) on the cell surface [11].Oligosaccharidic sequences of HS chains are de®ned for the bFGF binding and for the recognition of the speci®c bFGFreceptor, leading to the formation of a ternary ... the SECURIAprogram from the PackardInstrument Company (Meriden, CT, USA).DNA quanti®cation The DNA content of the cell layer at the end of incubationwas quanti®ed by the method of West et al .[31].Aftersolubilization ... are m ainlyregulated at the transcriptional level. This report s hows that the b FGF r egulation of their expression speci®cally dependson the nature of HSPG and of the Sertoli cell developmentalstage.In...

Tài liệu Báo cáo khoa học: Afﬁnity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx

... of data from indi-vidual binding curves indicated that the data weredescribed best by a two -binding site model. The IC50 (the concentration of the competing ligand that inhib-its 50% of the ... areassumed, then about 70% of the high-afﬁnity sites and about 12% of the low-afﬁnity sites are expected to be occupied at the concen-trations of unlabeled ligands used.Characterization of the binding ... lower-afﬁnity binding sites are listed in Table 1. The high-afﬁnity and the low-afﬁnity sites accountedfor 25% and 75% of the total binding, respectively.These estimates of the relative proportions of...

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... Approximately one-third of all mutations in the BTK gene are missenseand some of these reduce the stability of the protein.This is exempliﬁed by mutations in the BTK motif of the Tec homology (TH) ... identify individualswith mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humanshas ... Conse-quently, the PH–TH domain doublet of ITK fusesdirectly with the linker B kinase region of SYK. The PH domain of TFKs usually binds to phosphatidylino-sitol-3,4,5-trisphosphate, thereby bringing them...

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