Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... behav- ior by presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the critical value, the trajectory in the ... lipo- somes. The Src cycle can be started by the addition of relevant phosphatases (or other Src activators, such as the SH2/SH3-ligands) [38] to activate step...

Ngày tải lên: 18/02/2014, 11:20

... the peptide is bound in a concentrated manner at the top of the column, or, if bound to the resin prior to packing the column, the salt gradient concen- trates the peptide on its way out of the ... recom- binant Ab have also been made by several other groups, but most of these have relied on the generation of Ab fusions. Perhaps the best of these was reported...

Ngày tải lên: 18/02/2014, 13:20

... expect that the relative expression level of the GFP-tagged proteins is indicative of that of the equivalent untagged proteins. Lipid anchoring of C-Vrp1p bypasses the requirement for the LBD for ... 5A,B). Loss of the LBD abolished the ability of C-Vrp1p 364)817 to restore growth of vrp1D cells at elevated temperature. We next assessed the importance of the...

Ngày tải lên: 18/02/2014, 16:20

... was the same size as that of the translational product by isoform 2 mRNA (Fig. 4B). The product by isoform 1 mRNA was smaller than the isoform 2 product. The multiple bands may be explained by ... encoded another type of KLK11, isoform 2, despite the presence of exon 1c. The secretory path- ways of KLK11 isoforms appeared to differ, although both isoforms were secr...

Ngày tải lên: 19/02/2014, 06:20

... has Fig. 5. Analysis of the enzymatic activity of His-CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of 4¢-phosphopantothenoylcysteine (+ ... via the phospho- peptide-like compound 4¢-phosphopantothenoylcysteine. The synthesis of 4¢-phosphopantothenoylcysteine is cata- lyzed by the C-terminal CoaB domain of...

Ngày tải lên: 19/02/2014, 12:20

... decreases upon the binding of Ca 2+ . The change in the ratio of F340 ⁄ F380 determined the change in Ca 2+ concentration inside the cell. Acknowledgements This work was supported by The Norwegian ... mediates activation of Ras subsequent to the stimulation of G i - and G q - coupled receptors [8,9]. The main mechanism for the activation of Ras- GRF1 is the...

Ngày tải lên: 19/02/2014, 18:20

... the scale of the Bard’s genera- tivity, and the quality of its insights. 5 Empirical Evaluation The vastness of the web, captured in the large- scale sample that is the Google ngrams, means the Jigsaw ... acquired from the Web for our vocabulary of stereotypes (this point relates to the aptness of the similes sug- gested by the Bard); and second, that the...

Ngày tải lên: 20/02/2014, 05:20

... (HSPG) on the cell surface [11]. Oligosaccharidic sequences of HS chains are de®ned for the bFGF binding and for the recognition of the speci®c bFGF receptor, leading to the formation of a ternary ... the SECURIA program from the Packard Instrument Company (Meriden, CT, USA). DNA quanti®cation The DNA content of the cell layer at the end of incubation was quant...

Ngày tải lên: 21/02/2014, 03:20

... of data from indi- vidual binding curves indicated that the data were described best by a two -binding site model. The IC 50 (the concentration of the competing ligand that inhib- its 50% of the ... are assumed, then about 70% of the high-affinity sites and about 12% of the low-affinity sites are expected to be occupied at the concen- trations of unlabeled l...

Ngày tải lên: 14/02/2014, 14:20

... Approximately one- third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein. This is exemplified by mutations in the BTK motif of the Tec homology (TH) ... identify individuals with mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of d...

Ngày tải lên: 14/02/2014, 18:20