Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... corresponding to a substan- tial increase in k cat . Results Homeologous recombination of laccase- encoding sequences Chimeric laccase- encoding sequences were obtained in three independent homeologous intermolecular ... 2009) doi:10.1111/j.1742-4658.2009.07231.x Laccase- encoding sequences sharing 65–71% identity were shuffled in vivo by homeologous recombination. Yeast...

Ngày tải lên: 18/02/2014, 06:20

... & Rich DH (1988) Inhibition of porcine pepsin by two substrate analogues containing statine. The effect of histidine at the P2 subsite on the inhibition of aspartic proteinases. J Med Chem ... weaker inhibitor than those with P4 Asp. The polar interaction of Arg233 with the P3 Ser side chain hydroxyl of Ac-ESMD-cho is lost in one binding site. Also, the P2 Met in one of...

Ngày tải lên: 18/02/2014, 16:20

... 1343 where the relevant increase of m 2 produces the relevant intensity changes of ML ⁄ TG signals. In principle, such an increase can be due to both a decrease in c 1 and an increase in p 1 after growth ... dimension. A sine function was applied in both dimensions of the time domain before Fourier transformation. Integration of 1D and 2D signals was performed using 1D winnmr...

Ngày tải lên: 18/02/2014, 13:20

... functional link behind this finding is not clear. Interestingly, Ste20p and Cla4p also down-regulate sterol uptake by inhibiting the expression of genes involved in this process (Lin and Ho ¨ fken, ... morphology of the strains used in this study. (A) Morphology of deletion strains. The indicated strains were grown in YPD to stationary phase. The cells were then fixed with formald...

Ngày tải lên: 18/02/2014, 13:20

... judgments of the clarity of meaning of indivi- dual sentences. The approach through testing of reading comprehension provides a direct test of at least one aspect of the quality of translation. ... significance. DISCUSSION OF THE JUDGMENTS OF CLARITY TESTS The finding that mixing the types of translations during judging affects both types of translations, while lo...

Ngày tải lên: 19/02/2014, 19:20

... and apply phonological consistency in GPCs, including the roles of PCs in PSCs in Chinese, plays an instrumental role in learners’ competence in reading Chinese. Phono- logical consistency is ... remember the writing of Jhuyin symbols after the winter break. Hence, after the evaluation, we strengthened the feedback by adding Jhuyin information. In Fig- ure 2, the Jhuyi...

Ngày tải lên: 19/02/2014, 19:20

... [16,17] by modifying h-toxin (per- fringolysin O), a cholesterol-binding, pore-forming cytolysin produced by Clostridium perfringens [18]. Ch and BCh are noncytolytic derivatives of h -toxin that bind ... cholesterol by approximately 30% abolishes their binding to plasma membranes [17–19]. This is in remarkable contrast to cell binding by filipin, another cholesterol-binding reagen...

Ngày tải lên: 20/02/2014, 03:20

... how knowing the sort of each character is use- ful when tokenizing a string of characters into a string of words and when assigning parts -of- speech to them, and our method of clustering characters ... for an incrementally enlarged set of training data using or not us- ing character clustering. 15 Table 1 shows re- sults obtained from training sets of ATR-travel. The upp...

Ngày tải lên: 20/02/2014, 18:20

... (long-distance move- ment of the N-domain and rotational motion of the A-domain) of SERCA during the ATP-mediated ion transport reaction [14,16–19]. The single line scanning method [8,15], in which an AFM ... Ca 2+ was measured in the single line FSAFM images, and Fig. 1. Single-molecule imaging of SERCA dynamics in the presence of nucleotide and Ca 2+ . (A) Time-lapse sequenc...

Ngày tải lên: 14/02/2014, 14:20

... reperfusion injury [14]. In our studies, we employed GdCl 3 to inactivate KCs based on the findings of other investigators showing the destruction of KCs after the intravenous administra- tion of GdCl 3 [15]. ... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidative stress on the sur...

Ngày tải lên: 14/02/2014, 18:20