Tài liệu Báo cáo khoa học: Stefin A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc

... Stefin < /b> A < /b> displaces < /b> the < /b> occluding < /b> loop < /b> of < /b> cathepsin < /b> B only by as much as required to bind to the < /b> active site cleft Miha Renko, Urs ˇ ka Poz ˇ gan, Dus ˇ ana Majera and Dus ˇ an Turk Department ... showed that stefin < /b> A < /b> binds to cathepsin < /b> B as deeply as stefin < /b> B does to...

Ngày tải lên: 18/02/2014, 04:20

... phPGK1–5aa–cerulean were constructed using the < /b> primers 5¢-CCGGA ATTCC AATGT CGCTT TCTAA CAAGC T-3¢ and 5¢-GGCGG ATCCA TAATA TTGCT GAGAG CATCC A-< /b> 3¢, and 5¢- CCGGA ATTCC AATGT CGCTT TCTAA CAAGC T-3¢ and ... exposed to FLAG–affinity agarose beads (Sigma- Aldrich) that had been pretreated with antibody against FLAG. Proteins bound to washed beads were eluted, boiled, and separate...

Ngày tải lên: 16/02/2014, 09:20

... 5¢-AAAAGTGTGACATGGAATAAATT AGT-3¢ and 3¢-TTTTCACACTGTACCTTATTTAATCA-5¢; ICAP (28 bp), 5¢-AATTAATGTGACATATGTCACAT TAATT-3¢ and 3¢-TTAATTACACTGTATACAGTGTAAT TAA-5¢. The < /b> recognition half-sites are shown ... A < /b> ˚ . This distance decreases by about 8 A < /b> ˚ to 18.7 A < /b> ˚ on binding of < /b> cAMP to the < /b> anti-cAMP-binding sites of < /b> the < /b> protein. The...

Ngày tải lên: 20/02/2014, 23:20

... Petersburg, FL, USA) followed by PCR with Taq DNA polymerase (Promega, Madison, WT, USA) using the < /b> primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The < /b> cDNA ... regions, the < /b> overlapped part recognized as a < /b> putative transmembrane domain by all the < /b> programs is represented by a < /b> thick bar. The < /b> positio...

Ngày tải lên: 18/02/2014, 16:20

... consist of < /b> a-< /b> and b- subunits. Each subunit has a < /b> large extracellular region, a < /b> single transmembrane domain, and a < /b> short cytoplasmic tail (except for b 4 ). The < /b> N-terminal domains of < /b> the < /b> a-< /b> and b- subunits ... enhances cell–cell binding to suppress metastasis. J Biol Chem 271, 13811–13815. 70 Kitada T, Miyoshi E, Noda K, Higas...

Ngày tải lên: 18/02/2014, 17:20

... of < /b> AAP, as described in the < /b> Materials and methods. Completion of < /b> the < /b> hydrolysis was confirmed by the < /b> disappearance of < /b> the < /b> (Met1)-ONC (M23L) signal in the < /b> MALDI-TOF mass spectra 1 h after the < /b> addition ... above 9 . MALDI-TOF MS All mass spectra were acquired on a < /b> Bruker ULTRAFLEX TOF mass spectrometer, at the < /b> Serv...

Ngày tải lên: 19/02/2014, 12:20

... probability space of < /b> the < /b> translation model, as phrase-based translation probabilities are estimated using rela- tive frequencies. The < /b> new direct phrase-based translation probabilities are ... (“bad phrase-pairs”), (,) nbest Nfe % % equals 0, but the < /b> marginal count of < /b> f % is increased by () nbest Nf % , in this way the < /b> phrase-based transla-...

Ngày tải lên: 20/02/2014, 09:20

... the < /b> order it was observed. • Bigram probabilities . Finally, a < /b> natural ranking function, which has not been applied to the < /b> script event prediction task (but has 339 been applied to related tasks ... Next, statistics over these event chains are gathered and stored. Finally, the < /b> gathered statistics are used to create a < /b> model that takes as input a < /b...

Ngày tải lên: 22/02/2014, 02:20

... The < /b> association of < /b> p2 5a < /b> with brain ves- icles was demonstrated by subjecting a < /b> porcine brain homogenate to a < /b> vesicle flotation assay. The < /b> assay is based on the < /b> light vesicles float- ing in the < /b> ... potential to increase cytosolic DA by disturbing the < /b> balance between DAT and VMAT-2 and causing chronic oxidative stress due to...

Ngày tải lên: 14/02/2014, 21:20

... increased risk of < /b> atherosclerosis [21], focal ANGPTL2 secreted by vascular endothelial cells may be a < /b> mediator linking smoking to cardiovascular disease in an autocrine or paracrine manner. Blocking ANGPTL2 ... 26523–26528. 8 Ito Y, Oike Y, Yasunaga K, Hamada K, Miyata K, Matsumoto S, Sugano S, Tanihara H, Masuho Y & Suda T (2003) Inhibition of < /b> angiogenesis and va...

Ngày tải lên: 14/02/2014, 22:20