... purchased from Novagen (Madison, WI, USA).Restriction enzymes, modifying enzymes, and PCR enzymeswere from TaKaRa Bio (Kyoto, Japan). Crotalus atroxphosphodiesterase I was purchased from Sigma ... acidsequence similarity to RNase HI (Fig. 1). For example,it shows sequence identities of 27% to E. coli RNaseHI, 52% to C. glutamicum RNase HI, 31% to S. toko-daii RNase HI, and 38% to Halobacterium ... SCO2299 gene from Streptomyces coelicolor A3(2)encodes a bifunctional enzyme consisting of an RNase Hdomain and an acid phosphatase domainNaoto Ohtani1, Natsumi Saito1, Masaru Tomita1,...