Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... 3749 Proteolysis < /b> of < /b> Pseudomonas < /b> exotoxin < /b> A < /b> within < /b> hepatic < /b> endosomes < /b> by < /b> cathepsins < /b> B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects Tatiana El Hage 1,2 ,Se ´ verine Lorin 3 , ... blot analysis with an antibody against ETA showed that cathepsins < /b> B and D...

Ngày tải lên: 18/02/2014, 04:20

... glucan synthesis. Abbreviations DP, degree of < /b> polymerization; GBd, glucan-binding domain; GS, glucan-binding domain-deficient glucosyltransferase-I; GSd, glucansucrase domain; GSGB, glucosyltransferase-I ... sucrose hydrolysis, as described above and indicated by < /b> Kraij et al. [21] for reuteransucrase; and (b) ping-pong bi-bi A < /b> B Fig. 4. GTF activity as a < /b> func...

Ngày tải lên: 14/02/2014, 22:20

... baseline. (B) Slow waves recorded at two sites 6 mm apart along a < /b> strip before, during and after central applica- tion of < /b> 1 m M caffeine applied at widths of < /b> 3 and 5 mm. The 3 mm stream markedly increased ... higher than that of < /b> Ca 2+ [24]. However, a < /b> candidate mecha- nism that could serve as a < /b> coupling spring involves electrical membrane potentia...

Ngày tải lên: 16/02/2014, 09:20

... and disassembly of < /b> cadherin–catenin complexes The cytoplasmic domain of < /b> cadherin interacts with F-actin via b- catenin and a-< /b> catenin; b- catenin binds to cadherin and a-< /b> catenin, which in turn interacts ... Fujita and Yasuda [23] have suggested that this morphological difference is due to a < /b> change in cell–cell adhesion that is mediated by < /b>...

Ngày tải lên: 16/02/2014, 09:20

... polymerase a;< /b> DSBs, double strand breaks; DAPI, 4¢,6-diamidino-2-phenylindole dihydrochloride; BCAT, bovine catalase; YADH, yeast alcohol dehydrogenase. Enzymes: DNA-directed DNA polymerase (EC ... dehydrogenase (YADH: 7.4S), and bovine catalase (BCAT, 11.3 S). SDS/PAGE was perfomed and gel was stained with Coomassie Brilliant Blue (C). Each elution sample was analysed by < /b> SDS...

Ngày tải lên: 20/02/2014, 11:20

... responsible for binding and infection in E. coli.Thus, incorporation of < /b> a < /b> library of < /b> target proteins between the N-terminal domain and the C-terminal (CT) domain allows a < /b> protease-based selection because ... attempted a < /b> similar experiment to seek stable mutants of < /b> barnase. Using a < /b> subset of < /b> codons that only encode hydrophobic resid...

Ngày tải lên: 19/02/2014, 12:20

... purified SR and DOC- washed SR were stored in liquid nitrogen. The protein concentration in SR was determined with the Bradford protein assay (Bio- Rad, Hercules, CA, USA) calibrated by < /b> quantitative ... Typical single line scan data obtained with buffer conditions. (A)< /b> Representative single line scan graphs obtained at increasing ATP concentration in the range 0–100 l M...

Ngày tải lên: 14/02/2014, 14:20

... against hepatic < /b> drug-metabolizing dysfunction by < /b> modulation of < /b> the in ammatory response. Abbreviations ALT, alanine aminotrasferase; AST, aspartate aminotrasferase; CLP, cecal ligation and puncture; ... min and then incubated overnight at 4 °C with anti-CYP 1A1< /b> ⁄ 1A2< /b> serum (Abcam) with a < /b> con- stant rotation of < /b> the samples. Protein A < /b> ⁄...

Ngày tải lên: 14/02/2014, 18:20

... studies, cellular and animal models, and clinical studies. In addition to providing a < /b> basic understanding of < /b> the pro- cesses of < /b> protein folding and aggregation, such data help towards translational approaches ... and domain-swapped dimer as found in the structure of < /b> the tetramer (Protein Data Bank code: 1N9J); (B) stefin B monomer (Protein Data Bank co...

Ngày tải lên: 14/02/2014, 18:20

... containing MPTP (A < /b> and C) and MPP + (B and D) were analysed by < /b> SDS ⁄ PAGE (A < /b> and B) on 5–15% crosslinked polyacrylamide gel and western blotting (C and D) . (A,< /b> B) Lane M, prestained molecular mass ... eluates were pooled and the amount of < /b> protein was determined by < /b> the bicinchoninic acid assay [38] using bovine serum albumin as a...

Ngày tải lên: 14/02/2014, 19:20