Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... 100 lm of (GlcNAc) 1–4 was analysed at the start, in the middle and at the end of each series of sam- ples. The resulting average values of the standards (display- ing standard deviations of < ... exceeded in abundance by the structural biopoly- mers of plants (cellulose and hemicellulose) and is an important source of energy for a variety of organisms. The primar...

Ngày tải lên: 18/02/2014, 08:20

... al.[8],theshapeofthemoleculeresemblesa right hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms a thumb, and a loop between the B6 and B9 strands forms a cord (Fig. ... conformations of residues A10, B10 and A123 of CTX were refined. There were also signs of other conformations of surface residues A32, A37, A38, A62, A...

Ngày tải lên: 20/02/2014, 23:20

... factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles Zongwei Li, Hua Wei, Linzi Deng, Xiangfeng Cong and Xi Chen Research ... 2010) doi:10.1111/j.1742-4658.2010.07770.x To understand the potential paracrine roles of interleukin-1b (IL-1b), tumour necrosis factor-a (TNF...

Ngày tải lên: 18/02/2014, 04:20

... upregulation of ICAM-1 expression in Schwann cells may be important for host defenses after peripheral nervous system injury, and reducing the biosynthesis of ICAM-1 and other cytokines by blocking ... multiple steps of the CNS inflammation process, for example migration of leukocytes to inflammatory sites [14,15] and adhesion of polymorphonuclear cells and mono- c...

Ngày tải lên: 18/02/2014, 18:20

... Sciences, Turku, Finland). Estimation of expression of cytosolic regulatory proteins Total cell lysates of transfected CHO cells were used to esti- mate expression of Noxo1b, Noxo1c, and Noxo1a. The lysates ... (F) Expression of Noxo1b, Noxo1c, Nox1, and Noxa1 in various human cell lines: androgen-independent prostate cancer cells (LNCaP), androgen-independent prostate...

Ngày tải lên: 19/02/2014, 06:20

... tissues of leaves was only slightly detectable, and GUS still remained mostly in the stem and root vasculature (Fig. 2F). For 6-week old plants, the expression of AtHELPS in the vascular tissues of ... transport, in plants. Expression of AtHELPS is regulated by low and high K + To obtain clues about the molecular mechanisms of the regulation of AtHELPS expression,...

Ngày tải lên: 14/02/2014, 18:20

... contributions of the relBE TA system with respect to our understanding of the activity and function of the parD system. The basic structural information on all these systems and their functional ... the stability potential of the parD system was compared with that of the ccd system of plasmid F, as well as that of the parDE TA system of plasmid RK2 ⁄ RP4 and hok-sok o...

Ngày tải lên: 18/02/2014, 04:20

... [38], and the ccp 4 suite of programs [39] was used for scaling. Structure deter- mination for hACMSD was performed by means of the molecular replacement technique, using the coordi- nates of a ... ago [20], and very recently the existence of two isoforms originating by alternative splicing was demonstrated; although comparably expressed in various organs, only the hACMSD...

Ngày tải lên: 18/02/2014, 06:20

... after 16, 24 and 48 h of culture using trypan blue staining. (C) Cells were treated with 0.5, 1 and 2 lg of hpST3dODN and 1 and 2 lg of control ODN for 6 h or with lipids only; after 48 h of culture, ... cell death of SW480 cells. (A) SW480 cells were incubated in the absence of IFN-c or with 10, 100 and 200 ngÆmL )1 for 4, 24 and 48 h of incubation and c...

Ngày tải lên: 18/02/2014, 08:20

... conditions were 94 °C for 3 min followed by 39 cycles of 94 °C for 30 s, 65 °C for 1 min, 72 °C for 1 min and 72 °C for 10 min. This PCR product was used for the syn- thesis of VgR–dsRNA1. The targeted ... SiVgR-2.3-3-2, 5¢-ACAAGAGCCATTCTCTATGACGGTCTTTC-3¢, and SiVgR-2.3-4r, 5¢-CTGACCTGAGAGCGGATCAGATAT TATATTCAC-3¢, and the conditions were 94 °C for 3 min; 28 cycles...

Ngày tải lên: 18/02/2014, 08:20