Tài liệu Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems ppt

... toxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems Elizabeth Diago-Navarro 1 , Ana M. Hernandez-Arriaga 1 , ... stability potential of the parD system was compared with that of the ccd system of plasmid F, as well as that of the parDE TA syst...

Ngày tải lên: 18/02/2014, 04:20

... 100 lm of (GlcNAc) 1–4 was analysed at the start, in the middle and at the end of each series of sam- ples. The resulting average values of the standards (display- ing standard deviations of < ... 4MU-(GlcNAc) 2 as substrate; 50 lL of supernatant was mixed with 50 lLof 50 mm citrate–phosphate buffer, pH 3.8, containing 50 lm of substrate and 0.1 mgÆmL )1 of bovine...

Ngày tải lên: 18/02/2014, 08:20

... interaction of TS with AdoMet was observed in vitro with the enzyme isolated from the other enzymes of the system [10,1 3–1 6], suggesting that the allosteric interaction had a function in the control of Phser ... independent of what happens on the cystathionine side. The Phser branch-point combines the divergence of two fluxes (fluxes of cystathionine and threonine) with...

Ngày tải lên: 20/02/2014, 02:21

... cultures: aliquots of the cultures described above before and after the addition of inhibitor were obtained at 18 h and stained with Wright’s. The food vacuoles of parasites incubated with leupeptin ... and novel strategies in malaria therapy. Results and Discussion Inhibition of proteases by MA It has been previously proposed that MA may inhibit the activity of protease...

Ngày tải lên: 14/02/2014, 14:20

... activ- ity and maturation kinetics (both pH of activation and time of activation) of the two forms vary (Fig. 2A). Alternative splicing in plant systems has been reported [28] to affect the stability and ... Leu23 of the CT-extension of the rmErv-C +CT molecule with a minimum distance of 1.9 A ˚ between two atoms of the two leucines (Fig. 8A). Because the Leu23 residue...

Ngày tải lên: 14/02/2014, 14:20

... dur- ing its maturation, and about the relative contributions of Sco1 and Sco2 to this process. Our understanding of these and other events awaits the development of in vitro systems in which stabilized ... Cu(I) apoCox2 Cu(I)Sco2 Cu(I)Cox17 Cu(I) apoCox17 2S-S Cu(I)Cox17 Cu(I) Cu(I), 2e – Cu, 2e – apoCox17 2S-S Cytoplasm IMS Matrix Fig. 5. Pathway of copper insertion...

Ngày tải lên: 14/02/2014, 18:20

... protons of GlcNAc, and the H3-equitorial and H3-axial protons of N-glycolylneuraminic acid [2 3–2 6]. The 1 H-NMR spectrum of cRBCs within the region of interest is shown in Fig. 3 and the list of important ... presence of a mixture of both a2 fi 3 and a2 fi 6 linkages, with the peaks at 1.80 and 1.76 p.p.m. as a result of the H3 (axial) protons and peaks at 2.69...

Ngày tải lên: 14/02/2014, 19:20

... of the two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12]. The concentration of GTP in the cytoplasm of ... mM 81012 wtTorD – GTP wtTorD – XTP D68N – GTP D68N – XTP Fig. 6. TorD residue D68 controls substrate specificity. (A) The GTPase and XTPase activities of 0.1 m M...

Ngày tải lên: 16/02/2014, 09:20

... these factors. Consistent with a function of p107 and p130 in the control of CDE ⁄ CHR promoters is the loss of regula- tion observed for the expression of cyclin A, B-Myb and Cdc2 in p107 ) ⁄ ) p130 ) ... homologs of Caenor- habditis elegans synMuvB proteins and is composed of p130, E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54...

Ngày tải lên: 16/02/2014, 09:20

... post-transfection. VP2–EGFP and VP3–EGFP were highly toxic. As a control, cytotoxicity of VP 2– FLAG and VP3–FLAG was measured and found to be of comparable intensity to that of VP2–EGFP and VP3–EGFP (Fig. ... observed with the minor proteins fused with EGFP at their C-terminus (VP 3– EGFP and VP2–EGFP). By contrast, the minor struc- tural proteins fused with EGFP...

Ngày tải lên: 16/02/2014, 09:20