Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx

... FEBS Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry Jinn-Shiun Chen 1,2 , Kuei-Tien ... isobaric tags with related and absolute quantitation (iTRAQ) labeling and LC-MS ⁄ MS for quantitative analysis of the membrane proteome...

Ngày tải lên: 16/02/2014, 15:20

... that induction of apt1 and and induc- tion of gpx1 depend solely on the Pap1 and Atf1 tran- scription factors, respectively, and that induction of ctt1 is dependent on both Pap1 and Atf1 in Sc. ... The crude extract was analyzed by normal- phase TLC with authentic ubiquinone-10 as a standard. Normal- phase TLC was carried out on Kieselgel 60 F 254 with benzene. The band co...

Ngày tải lên: 18/02/2014, 14:20

... red balls and sticks. AB Fig. 3. S4 subsites of TEV (A) and TVMV (B) NIa proteases. Enzyme residues are shown with capped sticks, and the P4 residue of the substrate is shown with ball and stick representation. Comparison ... loss of selectivity (Table 3). AB Fig. 5. S2 (A) and S6 (B) subsites of TEV NIa protease. Enzyme residues are shown with capped sticks, and t...

Ngày tải lên: 19/02/2014, 16:20

... yielding means with corresponding SDs of estimated kinetic parameters. Identified values of V max matched the val- ues from experiments, and comparison of identified K m and K i values agreed with values ... interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the acce...

Ngày tải lên: 14/02/2014, 22:20

... roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss the possibility that both could func- tion as molecular targets for the prevention and treatment of obesity ... focus on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes- ity and related metabolic diseases, and discuss whether these ANGPTLs could be targets for the prevention and trea...

Ngày tải lên: 14/02/2014, 22:20

... chan- nel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues from the SET-C and post-SET regions. Lysine 4 of histone H3 is inserted ... domain is shown in blue (drawn from PDB code: 1sb0). Positions of E665 and E555 of the CBP–KIX domain, and residues K291 and R294 of the c-Myb transactivation do...

Ngày tải lên: 16/02/2014, 14:20

... after quenching of the reaction but prior to analysis is required. With this, the refolding of BPTI followed very similar kinetics in the presence or absence of baci- tracin, and after 40 min of refolding with ... chains of a folding protein or with the hydropho- bic binding site of molecular chaperones such as BiP, PDI and DsbC. To further study the mechanism of actio...

Ngày tải lên: 16/02/2014, 14:20

... disease. Synthesis and secretion of GIF in the brain Cellular and temporal localization of GIF synthesis Whilst the MT1 and MT2 isoforms are ubiquitously synthesized throughout most tissues in mammals, ... synthesis of GIF, albeit at seem- ingly much lower levels than within astrocytes. Neuro- nal synthesis of GIF is highly localized to specific subsets of cortical and hipp...

Ngày tải lên: 16/02/2014, 15:20

... stefin A and the different enzymes is the average distance between the centre of mass of CA atoms of the stefin molecule and the centre of mass of the CA atoms of the reactive site cysteine and histidine ... position. A comparison of the structure of the unli- ganded cathepsin B with the structure of the proenzyme, its complexes with chagasin and stefin A show...

Ngày tải lên: 18/02/2014, 04:20

... spectrum of protein 4, fraction 5 ⁄ fraction 6. Fig. S6. MS spectrum of protein 5, fraction 5 ⁄ fraction 6. Fig. S7. MS spectrum of protein 8, fraction 5 ⁄ fraction 6. Fig. S8. MS spectrum of protein ... MS spectrum of protein 9, fraction 1. Fig. S2. MS spectrum of protein 12, fraction 1. Fig. S3. MS spectrum of protein 14, fraction 1. Fig. S4. MS spectrum of...

Ngày tải lên: 18/02/2014, 08:20