Tài liệu Báo cáo khoa học: An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities doc

... An allosteric DNAzyme with dual RNA-cleaving and DNA-cleaving activities Dazhi Jiang*, Jiacui Xu*, Yongjie Sheng, Yanhong Sun and Jin Zhang Key Laboratory for Molecular Enzymology and Engineering ... Pb 2+ [14], and UO 2 2+ [15]; and constructing molecular logic gates and nanomotors [16–20]. How- ever, engineering an allosteric DNAzyme with dual RNA-clea...

Ngày tải lên: 16/02/2014, 15:20

... represent- ation only if it satisfies the Nonbranching Dominance, Uniqueness, Coherence and Com- pleteness conditions (Kaplan & Bresnan 1982). 2.2 Decomposition operations and Fragments The fragments for ... frontier nodes and deletes all subtrees they dominate. Like Root, it also removes the φ links of the deleted nodes and erases any semantic form that corresponds to any of thos...

Ngày tải lên: 20/02/2014, 18:20

... every opposite node V e i and V f j , associated with the function: ϕ rec i,j (k, l) =      1 if (i = l and j = k) or (i = l and j = k) 0 else We then connect a “translation probability” ... computing the English-side mes- sages and beliefs (m e ij and b e i ) , but the process must also be done symmetrically for the French- side messages and beliefs (m f ij and b f i )...

Ngày tải lên: 22/02/2014, 02:20

... is impor- tant and complete, cluster structure estimated from data subset in this feature space should be stable and robust against random sampling. After deter- mination of important contextual ... presented, which can be cate- gorized as feature filter (Dash et al., 2002; Talav- era, 1999) and feature wrapper (Dy and Brodley, 2000; Law et al., 2002; Mitra et al., 2002; Modha and Sp...

Ngày tải lên: 20/02/2014, 16:20

... thetical start and end of a reparandum (say from a language model such as (Heeman and Allen, 1997)), extends copies of phrase hy- potheses over the reparandum allowing the corrected utterance to ... ance and skips the reparandum. In the ex- ample, you should take the boxcar I mean the tanker to Coming; the reparandum is the boxcar. Based on our previous rules the edit- ing ter...

Ngày tải lên: 20/02/2014, 19:20

... membrane was then incubated for 1.5 h with a 1 : 25 000 dilution of anti-mouse IgG conjugated with Dy- Light 549 and anti-rabbit IgG conjugated with DyLight 649 (Pierce, Rockford, IL, USA), and ... USA) with an oligo-dT primer and random hexamer. PCR was per- formed with PCR master mix (Roche, Indianapolis, IN, USA) at 94 °C for 5 min followed by 35 cycles of 94 °C for 30 s,...

Ngày tải lên: 14/02/2014, 14:20

... 9500 g for 30 min, and the supernatant was decanted. The pellet was resuspended in 10 mL of supernatant, and then centrifuged at 20 000 g for 30 min. The supernatant was decanted, and 10 mL of 20 ... the activities of wild- type LF and LF-K518E ⁄ E682G in a cytotoxicity assay using this melanoma cell line. PA and either wild-type or mutant LF were incubated with MALME-3M cells f...

Ngày tải lên: 16/02/2014, 09:20

... localization of PBX1 and EXD proteins depends on nuclear import and export signals and is modulated by association with PREP1 and HTH. Genes Dev 13, 946–953. C. Hyman-Walsh et al. Meis2 transcriptional ... within these proteins that can mediate both positive and negative influences on transcriptional activity. Interestingly, splice variants of mammalian Meis1 and Meis2, and Dro...

Ngày tải lên: 16/02/2014, 15:20

... organizations and physiological func- tions: insulin is a heterodimeric peptide consisting of an A-chain and a B-chain, whereas IGFs are single- chain peptides with domains B, C, A and D, and ... in the female (C) and male (D) disks before and after cultivation for 5 days. Values are the means and standard errors of the mean (n = 6). (E–H) Confocal images of tissues with B...

Ngày tải lên: 18/02/2014, 13:20

... treatment with trichloroacetic acid (lanes 6, 8 and 9), and individual subunits were detected by silver staining (lane 6) or immunoblotting using an antibody against subunit c 1 (lanes 7 and 8) or ... Industrie (to BB), and the EU (grant NEST2004 PathSYS29084 to DM). We thank Deryck Mills for assistance with electron microscopy and Werner Ku ¨ hlbrandt (MPi of Biophysics, Fran...

Ngày tải lên: 18/02/2014, 17:20